Arginine Gene Clusters in the Proteus-Providence Group

Prozesky, O. W.; Grabow, W. O. K.; Merwe, Susanna Van Der; Coetzee, J. N.

doi:10.1099/00221287-77-1-237

Cited by 13 publications

(10 citation statements)

References 12 publications

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“…This kind of gene arrangement has been reported only in the Proteus species [21], and is different from that of E. coli [24], S. typhimurium [23], and perhaps from that of K. pneumoniae [16], since the argG loci of these latter species were not members of the cluster, and were located in a distant chromosomal region. Thus, it becomes apparent that the Proteus type of arg-gene cluster exists in bacteria not confined to the Proteus group.…”

Section: Discussionmentioning

confidence: 99%

“…In general, the arrangement of the structural genes for arginine biosynthesis in S. marcescens seems to be similar to that of the other species of Enterobacteriaceae, e.g., E. coli [24], S. typhimurium [23], Klebsiella pneumoniae [16], and Proteus species [20,21], although an exact conclusion on this subject must await complete biochemical and genetic analysis. This fact suggests the existence of a common ancestor from which these bacterial species have been derived.…”

Section: Discussionmentioning

confidence: 99%

“…1, and the genes participating in the arginine biosynthesis are scattered on the chromosome [20,23,24]. In Escherichia coli [24], Salmonella typhimurium [23] and Providencia [21], the four structural genes, i.e., argE, argC, argB and argH that code for the fifth, third, second and eighth enzymes, respectively, are clustered in this arrangement. In contrast with the three species, the analogous arggene clusters of Proteus mirabilis, Proteus vulgaris, Proteus rettgeri and Proteus morganii involve, in addition to the four genes, argG that codes for the seventh enzyme between argB and argH [21].…”

mentioning

confidence: 99%

“…In Escherichia coli [24], Salmonella typhimurium [23] and Providencia [21], the four structural genes, i.e., argE, argC, argB and argH that code for the fifth, third, second and eighth enzymes, respectively, are clustered in this arrangement. In contrast with the three species, the analogous arggene clusters of Proteus mirabilis, Proteus vulgaris, Proteus rettgeri and Proteus morganii involve, in addition to the four genes, argG that codes for the seventh enzyme between argB and argH [21]. Topographical knowledge on the arg genes in various species of Enterobacteriaceae is important from the phylogenic and taxonomic standpoints, since the arrangements of arg genes reflect the evolutionary trends in these bacterial species [12].…”

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Arginine Gene Cluster of Serratia marcescens

Matsui

Hosogaya

Suzuki

et al. 1975

Japanese Journal of Microbiology

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Biochemical and genetic studies on the arginine-requiring auxotrophs derived from a Serratia marcescens strain were carried out. The arg mutants were classified into seven biochemical groups based on their growth response to five precursors of arginine biosynthesis and enzyme deficiency. Reciprocal transduction tests among those arg mutants divided them into three linkage groups, and the fine mapping in each of the groups by two-or three-point crosses revealed the following arrangement of loci. (1) arg44-thy 11-lysl;(2) metl-glt2-arg E-(arg 19-arg51)-arg120-argG-argH ; (3) arg33-pyr4. Five of the seven biochemically distinct arg mutants belonged to the second linkage group, and they constituted an arg-gene cluster. A characteristic feature of the arg-gene cluster of S. marcescens is that it involves argG, which was previously reported only in the Proteus group of Enterobacteriaceae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Arginine Gene Cluster of Serratia marcescens

Matsui

Hosogaya

Suzuki

et al. 1975

Japanese Journal of Microbiology

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“…Host-controlled restriction and modification systems are operative in various strains of P. mirabilisincluding PMI 3 and ~~5 0 0 6 (Coetzee & Smit, 1969, 1970. For this reason the better-characterized arginine (Prozesky, 1968;Prozesky, Grabow, Van der Merwe & Coetzee, 1973), methionine (Grabow, 1 9 7 2~) and cysteine …”

Section: Discussionmentioning

confidence: 99%

Chromosome Transfer in Proteus Mirabilis Mediated by a Hybrid Plasmid

Coetzee

1975

Journal of General Microbiology

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A previously-described fused plasmid, P-ZacR 1drd19, was found to mediate chromosomal transfer between cells of Proteus mirabilis strain ~~5 0 0 6; ~~5 0 0 6 -(P-ZacRrdrd19) was usually the donor and various auxotrophs of ~~5 0 0 6 resistant to nalidixic acid and/or streptomycin were recipients. The donor was usually counterselected with nalidixic acid and/or high concentrgtions of streptomycin. Recombination experiments with single markers indicated a 40-fold variation in recombination frequencies for different markers. Mapping double-auxotrophic markers by their gradient of transmission confirmed this variation and placed each of two independent isolates of eight markers in a linkage group his-ser-ura-pyrBtrp-cys-ade-ilv. Some donor markers did not register. Despite low recombination frequencies, interrupted mating experiments showed a polarity of early marker transfer. The segregation of unselected markers confirmed the order of some markers and showed that genetic material passed from the presumptive donor to the recipient. Recipients with two auxotrophic markers which could not be cotransduced by phage 5006M were converted to prototrophy by conjugation. The plasmid transferred to recipients at high frequency and all recombinants carried it. Recombinants could act as donors in further matings. Recombinants were fully susceptible to phage 5006M, unlike transductants of ~~5 0 0 6 by this phage. Direct involvement of the plasmid was indicated by drastically diminished recombination frequencies in crosses with recipients carrying P-lac as resident. P-lac had previously been shown to reduce the frequency of transfer of the hybrid plasmid to cells harbouring it. The histidine region was the first to register in recipients and recombined at the highest frequency of 5 x Io-6/donor cell. Some temporary association of plasmid and perhaps only the histidine region of the chromosome is favoured as the mechanism of chromosomal transfer. This could explain why not all donor markers could be mapped. Transduction and transformation were excluded as the cause of results.

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The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa

Haas

Holloway

Schamböck³

et al. 1977

Molec. Gen. Genet.

171

121

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Six loci coding for arginine biosynthetic enzymes in Pseudomonas aeruginosa strain PAO were identified by enzyme assay: argA (N-acetylglutamate synthase), argB (N-acetylglutamate 5-phosphotransferase), argC (N-acetylglutamate 5-semialdehyde dehydrogenase), argF (anabolic ornithine carbamoyl-transferase), argG (argininosuccinate synthetase), and argH (argininosuccinase). One-step mutants which had a requirement for arginine and uracil were defective in carbamoylphosphate synthase, specified by a locus designated car. To map these mutations we used the sex factor FP2 in an improved interrupted mating technique as well as the generalized transducing phages F116L and G101. We confirmed earlier studies, and found no clustering of arg and car loci. However, argA, argH, and argB were mapped on a short chromosome segment (approx. 3 min long), and argF and argG were cotransducible, but not contiguous. N-Acetylglutamate synthase, the enzyme which replenishes the cycle of acetylated intermediates in ornithine synthesis of Pseudomonas, appears to be essential for arginine synthesis since argA mutants showed no growth on unsupplemented minimal medium.

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Arginine Gene Clusters in the Proteus-Providence Group

Cited by 13 publications

References 12 publications

Arginine Gene Cluster of Serratia marcescens

Arginine Gene Cluster of Serratia marcescens

Chromosome Transfer in Proteus Mirabilis Mediated by a Hybrid Plasmid

The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa

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