Argon Atmospheric Plasma Treatment Promotes Burn Healing by Stimulating Inflammation and Controlling the Redox State

Souza, Lucas Buzeli de; Silva, Jennyffer Ione de Souza; Bagne, Leonardo; Pereira, Amanda Tavares; Oliveira, Maraiara Aparecida de; Lopes, Bruno Bellotti; Amaral, Maria Esméria Corezola do; Aro, Andréa Aparecida de; Esquisatto, Marcelo Augusto Marretto; Santos, Gláucia Maria Tech dos; Andrade, Thiago Antônio Moretti de

doi:10.1007/s10753-020-01305-x

Cited by 10 publications

(9 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar effects of NIPP on TNF regulation have been observed in PDL cells using a different device (DBD) [ 32 ]. Moreover, in an animal model, NIPP also regulated TNF levels during wound healing at 2 d [ 48 ]. In contrast, in osteosarcoma cells TNF was not modulated by NIPP at 1 d [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Modulation of Inflammatory Responses by a Non-Invasive Physical Plasma Jet during Gingival Wound Healing

Eggers

Stope

Marciniak

et al. 2022

Cells

View full text Add to dashboard Cite

Gingival wound healing plays an important role in the treatment of a variety of inflammatory diseases. In some cases, however, wound healing is delayed by various endogenous or exogenous factors. In recent years, non-invasive physical plasma (NIPP), a highly reactive gas, has become the focus of research, because of its anti-inflammatory and wound healing-promoting efficacy. So far, since NIPP application has been poorly elucidated in dentistry, the aim of this study was to further investigate the effect of NIPP on various molecules associated with inflammation and wound healing in gingival cells. Human gingival fibroblasts (HGF) and human gingival keratinocytes (HGK) were treated with NIPP at different application times. Cell viability and cell morphology were assessed using DAPI/phalloidin staining. Cyclooxygenase (COX)2; tumour necrosis factor (TNF); CC Motif Chemokine Ligand (CCL)2; and interleukin (IL)1B, IL6 and IL8 were analysed at the mRNA and protein level by a real-time PCR and ELISA. NIPP did not cause any damage to the cells. Furthermore, NIPP led to a downregulation of proinflammatory molecules. Our study shows that NIPP application does not damage the gingival tissue and that the promotion of wound healing is also due to an anti-inflammatory component.

show abstract

Section: Discussionmentioning

confidence: 99%

Modulation of Inflammatory Responses by a Non-Invasive Physical Plasma Jet during Gingival Wound Healing

Eggers

Stope

Marciniak

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

“…Then, after having only the immunohistochemistry color on each image, this plugin can quantify only the strongest tones of immunohistochemistry color, not considering the background (weakest tones). Once IL‐10 and VEGF can be found intra‐ and extra‐cellularly, it was quantified the % of total immunolabeling present in the connective tissue for these markers [18–21].…”

Section: Methodsmentioning

confidence: 99%

“…In each well of the plate, 30 μl of substrate 3, 3′, 5, 5′‐tetramethylbenzidine (TMB) was added (Sigma Chemical Company, St. Louis, MO), followed by 100 μl of H 2 O 2 . The reaction was stopped with 4 M sulfuric acid and read in a plate reader at 450 nm [19, 20]. The results were expressed as optical density (O. D.) of MPO/mg tissue.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Photobiomodulation and photodynamic therapy applied after electrocauterization for skin healing optimization in rats

Manoel

Mariano

Ramos

et al. 2022

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

Photobiomodulation-PBM and Photodynamic Therapy-PDT have been used to induce healing. However, the effects of these therapies on skin-lesions induced by electrocautery are unknown, aiming at more favorable clinical and esthetic results. Electrocauterization was done in 78-female Wistar-rats using a system that includes an electrocautery and red-LED. The groups were: No injury, Injury, Injury + ALA (topical 5-aminolevulinic acid application), Injury + LED and Injury + ALA + LED (topical ALA application followed by photoactivation with LED). After 2nd, 7th and 14th days post-injury, immuno-histomorphometric analyses (inflammatory infiltrate, blood vessels, fibroblasts, eschar/epidermal thickness, IL-10 and VEGF) and biochemical assays of MPO (neutrophil), NAG (macrophage), nitrite, DCF (H 2 O 2 ), carbonyl (membrane's damage), sulfhydryl (membrane's integrity), SOD, GSH, hydroxyproline and re-epithelialization area were performed. The Injury + LED and Injury + ALA + LED groups controlled inflammation and oxidative stress, favoring angiogenesis, fibroblasts proliferation and collagen formation.

show abstract

“…Apart from non-infected wounds, augmented healing was also observed in gas plasma-treated infected wounds [ 100 ]. Mechanistically, one study identified an increase of free thiols and neutrophil activity, as observed by increased cellular influx and myeloperoxidase (MPO) levels [ 98 ]. Gas plasma was also used as an enabling technology, functionalizing polypropylene particles to increasingly absorb betaine hydrochloride, which improved wound healing in diabetic rats [ 102 ].…”

Section: Gas Plasma Treatment Of Skin and Wounds In Animal And Veterinary Modelsmentioning

confidence: 99%

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

2021

View full text Add to dashboard Cite

The loss of skin integrity is inevitable in life. Wound healing is a necessary sequence of events to reconstitute the body’s integrity against potentially harmful environmental agents and restore homeostasis. Attempts to improve cutaneous wound healing are therefore as old as humanity itself. Furthermore, nowadays, targeting defective wound healing is of utmost importance in an aging society with underlying diseases such as diabetes and vascular insufficiencies being on the rise. Because chronic wounds’ etiology and specific traits differ, there is widespread polypragmasia in targeting non-healing conditions. Reactive oxygen and nitrogen species (ROS/RNS) are an overarching theme accompanying wound healing and its biological stages. ROS are signaling agents generated by phagocytes to inactivate pathogens. Although ROS/RNS’s central role in the biology of wound healing has long been appreciated, it was only until the recent decade that these agents were explicitly used to target defective wound healing using gas plasma technology. Gas plasma is a physical state of matter and is a partially ionized gas operated at body temperature which generates a plethora of ROS/RNS simultaneously in a spatiotemporally controlled manner. Animal models of wound healing have been vital in driving the development of these wound healing-promoting technologies, and this review summarizes the current knowledge and identifies open ends derived from in vivo wound models under gas plasma therapy. While gas plasma-assisted wound healing in humans has become well established in Europe, veterinary medicine is an emerging field with great potential to improve the lives of suffering animals.

show abstract

Argon Atmospheric Plasma Treatment Promotes Burn Healing by Stimulating Inflammation and Controlling the Redox State

Cited by 10 publications

References 58 publications

Modulation of Inflammatory Responses by a Non-Invasive Physical Plasma Jet during Gingival Wound Healing

Modulation of Inflammatory Responses by a Non-Invasive Physical Plasma Jet during Gingival Wound Healing

Photobiomodulation and photodynamic therapy applied after electrocauterization for skin healing optimization in rats

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

Contact Info

Product

Resources

About