Arjunolic Acid Improves the Serum Level of Vitamin B12 and Folate in the Process of the Attenuation of Arsenic Induced Uterine Oxidative Stress

Maity, Moulima; Perveen, Hasina; Dash, Moumita; Jana, Suryashis; Khatun, Shamima; Dey, Arindam; Mandal, Amit Kumar; Chattopadhyay, Sandip

doi:10.1007/s12011-017-1077-0

Cited by 23 publications

(10 citation statements)

References 76 publications

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“…In ovarian steroidogenesis 3 and 17 -HSDs are the key regulatory enzymes, the suppressed activities of these enzymes along with elevated cholesterol levels by arsenic suggest alteration in its hormone synthesis 27,30,31 . This is in agreement with the previous findings where arsenic treatment was associated with inhibition of ovarian steroidogenesis 27,32,33 . To minimize the risk caused by ROS, supplementation of Andrographis paniculata in arsenic-treated mice resulted in a significant increase in ovarian enzyme activities and reduction in cholesterol levels.…”

Section: Resultssupporting

confidence: 94%

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The present study was designed to determine the role of Andrographis paniculata (AP; 50 mg/kg) against the oxidative stress caused by the arsenic (0.5 and 1.0 mg/kg) for 30 days in adult mice. The results of in-vivo studies demonstrated that arsenic treatment resulted in a significant dose-dependent increase in the ovarian arsenic level and lipid peroxidation (LPO), followed by a marked reduction in gravimetric data, levels of total ascorbic acid, glutathione and in the activities of superoxide dismutase, catalase, and glutathione peroxidase. A significant reduction in the activities of 3 and 17 hydroxysteroid dehydrogenases along with elevated levels of cholesterol indicated ovarian endocrine dysfunction. These results suggest that an increase in free radical formation relative to loss of antioxidant defense system during arsenic exposure may render ovary more susceptible to oxidative damage leading to their functional inactivation. On the other hand, co-administration of arsenic with Andrographis paniculata (AP) restored altered biochemical variables, antioxidant balance, and gravimetry which suggests that the free radical-mediated damage during arsenic-induced oxidative stress could have been ameliorated by A. paniculata (AP) by its anti-oxidative potential.

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Section: Resultssupporting

confidence: 94%

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2019

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“…1. These results are similar with the other investigator, where the arjunolic acid 31 and vitamin B 12 32 could prevent the inhibition in the activities of SOD, catalase, and GPx in arsenicated rats.…”

Section: Discussionsupporting

confidence: 92%

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2019

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The present study was proposed to delineate the curative role of arjunolic acid and vitamin B 12 alone or in combination against arsenic induced oxidative stress and female reproductive dysfunction. The Wistar strain adult female rats were treated with sodium arsenite at the dose of 1.0 mg/100 gm body weight orally for first eight days. Next eight days rats were treated with arjunolic acid (1.0 mg/100 gm body weight) and vitamin B 12 (0.09 μg/100 gm body weight) orally. In ovarian and uterine tissue showed a recovery in arsenic induced ROS-production following the post-treatment of arjunolic acid and vitamin B 12. Antioxidant status was also improved by arjunolic acid and vitamin B 12 as established from the electrozymographic analysis of SOD, catalase and GPx. Post-treatment of these two significantly retrieved ovarian estradiol receptor 1 (ESR-1) from arsenic induced down regulation. Arjunolic acid and vitamin B 12 also showed its curative ability in improving the level of inflammatory markers such as NF-κB, metallothioneine-1 (MT-1), TNF-α, IL-6. Thus these two bioactive substances might have the curative role against arsenic induced female reproductive dysfunction. INTRODUCTION: The trioxide form of arsenic has the ability to react with cellular thiols to exert its toxicity and during the metabolism of thiols in cells reactive oxygen species generates which thereby promotes tissue damage 1. Arsenic is the cause of several reproductive hazards. It impairs spermatogenesis process and decreases the spermatogenetic enzymes in male reproductive system. The spermatogenic meiosis and the postmeiotic stages were altered by arsenic. Gonadal dysfunctions like declined testosterone synthesis, apoptotic and necrotic changes due to arsenic mediated oxidative damage 2 may cause male infertility.

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“…However, arsenic at a dose of 10 mg/kg body mass for a short duration exhibited a significant level of free radical generation in the organs (Manna et al 2007;Sinha et al 2008;Bhattacharya and Haldar 2013). Arsenic treatment at a dose of 10 mg/kg body mass for a short duration like 8 d was previously used by our group (Maity et al 2018). In this experiment, arsenic (As III ) was involved in the generation of oxidative stress and manifested in elevated uterine MDA and CD levels (Fig.…”

Section: Discussionmentioning

confidence: 78%

Spirulina platensisameliorates arsenic-mediated uterine damage and ovarian steroidogenic disorder

Khatun

Maity

Perveen

et al. 2018

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Novel, non-invasive, painless oral therapeutic agents are needed to replace the painful conventional treatment of arsenic-associated health hazards with metal chelators. Our aim was to examine the effect of spirulina ( Spirulina platensis ( Geitler, 1925 )) on arsenic-mediated uterine toxicity. Female Wistar rats were divided equally into four experimental treatment groups: control group, sodium arsenite group (1.0 mg/100 g body mass), spirulina placebo group (20 mg/100 g body mass), and sodium arsenite + spirulina group. In contrast with the control group, spectrophotometric and electrozymographic evaluation revealed that rats that ingested arsenic for 8 d showed significant diminution of the activities of superoxide dismutase, catalase, and peroxidase ( p < 0.001). Mutagenic uterine DNA breakage and tissue damage were prominent following arsenic consumption by the rats. Oral delivery of spirulina resulted in a significant amelioration of arsenic-induced adverse oxidative stress and genotoxic state of rats. A significant low-signaling ( p < 0.001) of gonadotropins and estradiol was also noted in the arsenic-treated rats, which was terminated by spirulina; this arsenic-primed adverse effect was significant ( p < 0.05, p < 0.01). The spirulina treatment mechanism could be associated with augmentation of the antioxidant defense system that protects the arsenic-mediated pathological state of the uterus.

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Arjunolic Acid Improves the Serum Level of Vitamin B12 and Folate in the Process of the Attenuation of Arsenic Induced Uterine Oxidative Stress

Cited by 23 publications

References 76 publications

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Spirulina platensisameliorates arsenic-mediated uterine damage and ovarian steroidogenic disorder

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