Arl13b controls basal cell stemness properties and Hedgehog signaling in the mouse epididymis

Girardet, Laura; Cyr, Daniel G.; Belleannée, Clémence

doi:10.1007/s00018-022-04570-1

Cited by 7 publications

(4 citation statements)

References 71 publications

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“…The different medium formulas can be responsible for the morphological diversity, but both medium managed to culture primary hAECs into BCs. According to earlier research (26), KRT5 and P63 are markers showing cell stemness. Differentiated cells or cells ready to differentiate will lose these stemness markers.…”

Section: Discussionmentioning

confidence: 90%

A novel type of serum‑free medium for culturing human airway epithelium cells

et al. 2023

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The present study aimed to evaluate the ability of a novel serum-free medium (SFM) to culture human airway epithelium cells (hAECs). hAECs were cultured in the novel SFM as the experimental group in the PneumaCult-Ex medium and Dulbecco's modified eagle medium (DMEM) and fetal bovine serum (FBS) as the control groups. Cell morphology, proliferative capacity, differentiation capacity and expression levels of basal cell markers were assessed accordingly in both culture systems. Optical microscope photos of hAECs were collected for cell morphology assessment. Cell Counting Kit-8 assay was conducted to assess the proliferation ability, and an air-liquid interface (ALI) assay was conducted to assess the differentiation capacity. Markers for proliferating basal and differentiated cells were relatively identified by immunohistochemical and immunofluorescent analysis. The results show that whether grown in the novel SFM or Ex medium, hAECs exhibited similar morphology at every passage, whereas cells could hardly form colonies in the DMEM + FBS group. Cells typically exhibited cobblestone shape, while a proportion of them in the novel SFM at late passage exhibited a larger shape. White vesicles appeared in the cytoplasm of some control cells at the later stage of culture. Basal cell markers (P63 + KRT5 + KI67 + CC10 -) for proliferating ability were found in the hAECs cultured by the novel SFM and Ex medium. hAECs at passage 3 cultured in the novel SFM and Ex medium both had the capacity to differentiate into ciliated cells (acetylated tubulin + ), goblet cells (MUC5AC + ) and club cells (CC10 + ) in the ALI culture assay. In conclusion, the novel SFM was capable of culturing hAECs. The hAECs cultured by the novel SFM could proliferate and differentiate in vitro. The novel SFM does not change the morphological characteristics or biomarkers of hAECs. The novel SFM has the potential for the amplification of hAECs for scientific research and clinical application.

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Section: Discussionmentioning

confidence: 90%

A novel type of serum‑free medium for culturing human airway epithelium cells

et al. 2023

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“…Primary cilia were observed on both surfaces of basal cells as well as within intercellular spaces. Damage to these primary cilia on basal cells lead to imbalanced apoptosis rates among epididymal epithelial cells, thus highlighting their role as guardians for maintaining homeostasis within pseudostratified epithelia ( Girardet et al, 2022 ).…”

Section: Distinct Cellular Compositions Across Different Epididymal R...mentioning

confidence: 99%

The significance of single-cell transcriptome analysis in epididymis research

Liu,

Feng,

et al. 2024

Front. Cell Dev. Biol.

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As a crucial component of the male reproductive system, the epididymis plays multiple roles, including sperm storage and secretion of nutritive fluids for sperm development and maturation. The acquisition of fertilization capacity by sperm occurs during their transport through the epididymis. Compared with the testis, little has been realized about the importance of the epididymis. However, with the development of molecular biology and single-cell sequencing technology, the importance of the epididymis for male fertility should be reconsidered. Recent studies have revealed that different regions of the epididymis exhibit distinct functions and cell type compositions, which are likely determined by variations in gene expression patterns. In this research, we primarily focused on elucidating the cellular composition and region-specific gene expression patterns within different segments of the epididymis and provided detailed insights into epididymal function in male fertility.

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“…Narrow and clear cells actively secrete protons into the lumen, thereby lowering the fluid pH, which helps to maintain the maturing sperm in a non-motile state ( 144 ). The basal cells have stem cell features and may be able to regenerate the epithelium ( 145 ). Most importantly, they have axiopodia that can reach the lumen in some regions and participate in transepithelial transport and physiological cross-talk with the clear cells ( 144 ).…”

Section: Male Reproductive System and Potential Targets For Steriliza...mentioning

confidence: 99%

Male animal sterilization: history, current practices, and potential methods for replacing castration

Hess,

Park,

Soto

et al. 2024

Front. Vet. Sci.

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Sterilization and castration have been synonyms for thousands of years. Making an animal sterile meant to render them incapable of producing offspring. Castration or the physical removal of the testes was discovered to be the most simple but reliable method for managing reproduction and sexual behavior in the male. Today, there continues to be global utilization of castration in domestic animals. More than six hundred million pigs are castrated every year, and surgical removal of testes in dogs and cats is a routine practice in veterinary medicine. However, modern biological research has extended the meaning of sterilization to include methods that spare testis removal and involve a variety of options, from chemical castration and immunocastration to various methods of vasectomy. This review begins with the history of sterilization, showing a direct link between its practice in man and animals. Then, it traces the evolution of concepts for inducing sterility, where research has overlapped with basic studies of reproductive hormones and the discovery of testicular toxicants, some of which serve as sterilizing agents in rodent pests. Finally, the most recent efforts to use the immune system and gene editing to block hormonal stimulation of testis function are discussed. As we respond to the crisis of animal overpopulation and strive for better animal welfare, these novel methods provide optimism for replacing surgical castration in some species.

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Arl13b controls basal cell stemness properties and Hedgehog signaling in the mouse epididymis

Cited by 7 publications

References 71 publications

A novel type of serum‑free medium for culturing human airway epithelium cells

A novel type of serum‑free medium for culturing human airway epithelium cells

The significance of single-cell transcriptome analysis in epididymis research

Male animal sterilization: history, current practices, and potential methods for replacing castration

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