ARMS test for diagnosis of factor VLeidenmutation and allele frequencies in France

Bathelier, C.; Champenois, T.; Lucotte, G.

doi:10.1006/mcpr.1997.0152

Cited by 15 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the ARMS‐method was concordant with RFLP‐results on 196 DNA samples 53. In another study, the ARMS method and RFLP were compared by genotyping 67 FV WT and 108 FVL heterozygote samples; the results were identical except for one sample which was typed as heterozygote by RFLP, but FVL homozygote by ARMS 54. The ARMS assay can be designed to test for a single SNP in a multiallelic system.…”

Section: Dna Assaysmentioning

confidence: 89%

See 1 more Smart Citation

Evolving methods for single nucleotide polymorphism detection: Factor V Leiden mutation detection

Oh¹,

Smith²

2011

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

Section: Dna Assaysmentioning

confidence: 89%

“…The transfer is distance dependent, i.e., the donor and the acceptor must be within 10–1,000 nm. FRET Q‐PCR with melting curve analysis based genotyping using allele‐specific fluorescence to differentiate PCR products by T m characteristics, and was shown to be as reliable as RFLP analysis 54, 55.…”

Section: Dna Assaysmentioning

confidence: 99%

Evolving methods for single nucleotide polymorphism detection: Factor V Leiden mutation detection

Oh¹,

Smith²

2011

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…DNA for this purpose is most reliably produced from fresh blood samples, or from samples stored frozen at −20 °C. An ARMS assay for FVL was developed by Bathelier, Champenois and Lucotte (1998), who considered this method to be reliable, and simpler than RED. The PCR product was electrophoresed on agarose mini‐gels.…”

Section: Detection Of Fvl and Pt G20210amentioning

confidence: 99%

An overview of methods for detection of factor V Leiden and the prothrombin G20210A mutations

Cooper

Rezende

2007

Int J Lab Hematology

View full text Add to dashboard Cite

Summary Venous thromboembolism, represented by deep venous thrombosis and pulmonary embolism, is a common disease with high mortality and morbidity. Within the last 25 years, risk factors for venous thromboembolism have been linked to mutations in the genes of the coagulation/anticoagulation system. Factor V Leiden and the prothrombin G20210A mutations are the most prevalent inherited risk factors predisposing to venous thromboembolism in the Western world. Tests to detect these mutations are carried out when investigating a personal or family history of venous thromboembolism. At the present, there are several different methods available for the detection of these mutations in the laboratory. The choice of the method will depend on many variables. This article is aimed at reviewing the available methods for the detection of factor V Leiden and prothrombin G20210A mutations, their principle, applicability, advantages and disadvantages of use.

show abstract

High prevalence of three prothrombotic polymorphisms among Palestinians: factor V G1691A, factor II G20210A and methylenetetrahydrofolate reductase C677T

Hussein

2012

J Thromb Thrombolysis

View full text Add to dashboard Cite

Factor V leiden G1691A/R506Q (FVL), prothrombin G20210A (FII) and methylenetetrahydrofolate reductase (MTHFR) C677T are related genetic risk factors for venous thromboembolism. Analysis for those mutations is increasingly being performed on patients exhibiting hypercoagulability. The objective of this study was to determine the prevalence of FVL, FII-G20210A and MTHFR-C677T polymorphisms and their coexistence among apparently healthy Palestinians. After institutional approval, 303 apparently healthy students from An-Najah University representative to North and South regions of West Bank with no previous history of cardiovascular diseases participated in this study. A uniform questionnaire was used to collect relevant information through personal interview with the subjects. The collected information included gender, age, smoking habits, weight and height, diseases such as diabetes, cardiovascular and family history of CVD. The frequencies of allelic distribution of the three prothrombotic polymorphisms factor V G1691A/R506Q), prothrombin G2010A, and MTHFR-C677T were 0.114, 0.050 and 0.071, respectively. The prevalence of the three thrombotic polymorphisms (FVL, FII G20210A and MTHFR-C677T) were 20.1, 9.1 and 13.8 %, respectively. Statistical analysis for factor V leiden showed no significant association between place of residence (P value = 0.953) and gender (P value >0.082). The data presented in this study showed the highest prevalence of FVL among healthy Palestinians compared to other populations and this important finding should be followed in terms of clinical significance.

show abstract

ARMS test for diagnosis of factor VLeidenmutation and allele frequencies in France

Cited by 15 publications

References 0 publications

Evolving methods for single nucleotide polymorphism detection: Factor V Leiden mutation detection

Evolving methods for single nucleotide polymorphism detection: Factor V Leiden mutation detection

An overview of methods for detection of factor V Leiden and the prothrombin G20210A mutations

High prevalence of three prothrombotic polymorphisms among Palestinians: factor V G1691A, factor II G20210A and methylenetetrahydrofolate reductase C677T

Contact Info

Product

Resources

About