Aromatic Surfactant as Aggregating Agent for Aptamer-Gold Nanoparticle-Based Detection of Plasmodium Lactate Dehydrogenase

Jain, Priyamvada; Chakma, Babina; Singh, Naveen K.; Patra, Sanjukta; Goswami, Pranab

doi:10.1007/s12033-016-9946-x

Cited by 40 publications

(21 citation statements)

References 36 publications

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“…[89], Plasmodium spp. [90,91,92,93,94,95,96,97,98,99], Cryptosporidium parvum [100], Entamoeba histolytica [101]. A more thorough overview of the recent advances on aptamers as diagnostics of protozoan parasites was reviewed by Ospina-Villa et al [73].…”

Section: Applications Of Aptamersmentioning

confidence: 99%

Recent Advances in Aptamer Discovery and Applications

2019

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Aptamers are short, single-stranded DNA, RNA, or synthetic XNA molecules that can be developed with high affinity and specificity to interact with any desired targets. They have been widely used in facilitating discoveries in basic research, ensuring food safety and monitoring the environment. Furthermore, aptamers play promising roles as clinical diagnostics and therapeutic agents. This review provides update on the recent advances in this rapidly progressing field of research with particular emphasis on generation of aptamers and their applications in biosensing, biotechnology and medicine. The limitations and future directions of aptamers in target specific delivery and real-time detection are also discussed.

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Section: Applications Of Aptamersmentioning

confidence: 99%

Recent Advances in Aptamer Discovery and Applications

2019

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“…There is a critical need for a better understanding of malaria as well as tests for the rapid diagnosis and surveillance of malaria. As such, there have been many reports describing the selection of new aptamers 3,4,11,[58][59][60][61] to malaria targets as well as novel aptamer-based biosensors [62][63][64] . Previous studies generating aptamer probes to the IE surface have used pre-defined Plasmodium recombinant protein fragments or intact IEs and sophisticated microfluidic fabrication.…”

Section: Discussionmentioning

confidence: 99%

High-efficiency enrichment enables identification of aptamers to circulating Plasmodium falciparum-infected erythrocytes

Oteng

McKeague

2020

Sci Rep

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Plasmodium falciparum is the causative agent of the deadliest human malaria. New molecules are needed that can specifically bind to erythrocytes that are infected with P. falciparum for diagnostic purposes, to disrupt host-parasite interactions, or to deliver chemotherapeutics. Aptamer technology has the potential to revolutionize biological diagnostics and therapeutics; however, broad adoption is hindered by the high failure rate of the systematic evolution of ligands by exponential enrichment (SELEX). Here we performed parallel SELEX experiments to compare the impact of two different methods for single-strand recovery on the efficiency of aptamer enrichment. Our experimental results and analysis of SELEX publications spanning 13 years implicate the alkaline denaturation step as a significant cause for inefficient aptamer selection. Thus, we applied an exonuclease single-strand recovery step in our SELEX to direct aptamers to the surface of erythrocytes infected with P. falciparum. The selected aptamers bind with high affinity (low nanomolar K d values) and selectivity to exposed surface proteins of both laboratory parasite strains as well isolates from patients in Asia and Africa with clinical malaria. The results obtained in this study potentially open new approaches to malaria diagnosis and surveillance. Malaria is a global health problem, with 228 million clinical episodes and an estimated 405,000 deaths each year 1. Plasmodium falciparum is the causative agent of the deadliest human malaria. A constellation of parasite-encoded proteins is displayed on the surface of infected erythrocytes (IEs) that are important in the life cycle of the parasite and mediate critical host-parasite interactions. One important host-parasite interaction is IE sequestration in the microvasculature which is thought to lead to the most severe manifestations of the disease. Therefore, ligand-binding molecules that can specifically identify and bind to IEs may disrupt important host-parasite interactions, deliver chemotherapeutics to parasitized cells, and have potential to further our understanding of the diversity of proteins expressed on the IE surface 2-5. Indeed, antibodies targeting IEs disrupt pathogenesis and contribute to naturally-acquired immunity to malaria 6-9. Aptamers are an alternative to antibodies that may sufficiently serve as molecular recognition agents of IEs. Several aptamers have been selected that bind to targets and biomarkers of malaria infection (see review 10 for examples). Aptamers are single-stranded nucleic acids that bind target molecules with antibody-like affinity and specificity through shape complementarity 11,12. However, the nucleic acid composition of aptamers presents several advantages over antibodies including increased stability, cell-free synthesis (either by PCR or chemical synthesis), and simple modification for detection and immobilization. Aptamers have been developed against small molecules, peptides, proteins, and whole cells including bacteria and parasites 3,13-16. As such, aptame...

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“…Table 1 summarizes and compares recent studies on the diagnosis of PfLDH. Recently, many studies have developed high-sensitivity, high-selectivity, and cost-effective diagnostics based on aptamers or nanoparticles [31][32][33][34][35]. Nanoparticles, such as novel-metal nanoparticles and magnetic nanoparticles, are applied for lateral flow immunoassay or combined with aptamers to improved diagnostic performance.…”

Section: Performance Of the Microfluidic Microplate For Pfldh Immunoamentioning

confidence: 99%

Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

et al. 2020

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This work reports on a rapid diagnostic platform for the detection of Plasmodium falciparum lactate dehydrogenase (PfLDH), a representative malaria biomarker, using a microfluidic microplate-based immunoassay. In this study, the microfluidic microplate made it possible to diagnose PfLDH with a small volume of sample (only 5 μL) and short time (< 90 min) compared to conventional immunoassays such as enzyme-linked immunosorbent assay (ELISA). Moreover, the diagnostic performance of PfLDH showed high sensitivity, specificity, and selectivity (i.e., 0.025 pg/μL in phosphate-buffered saline and 1 pg/μL in human serum). The microfluidic-based microplate sensing platform has the potential to adapt simple, rapid, and accurate diagnoses to the practical detection of malaria.

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Aromatic Surfactant as Aggregating Agent for Aptamer-Gold Nanoparticle-Based Detection of Plasmodium Lactate Dehydrogenase

Cited by 40 publications

References 36 publications

Recent Advances in Aptamer Discovery and Applications

Recent Advances in Aptamer Discovery and Applications

High-efficiency enrichment enables identification of aptamers to circulating Plasmodium falciparum-infected erythrocytes

Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

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