Array-Based Screening of Silver Nanoparticle Mineralization Peptides

Abstract

The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthesis of nanoscale materials. Silver nanoparticle (AgNP) mineralization using peptides has been investigated; however, further comprehensive analysis must be carried out, because the design of peptide mediated-AgNP properties is still highly challenging. Herein, we employed an array comprising 200 spot synthesis-based peptides, which were previously isolated as gold nanoparticle (AuNP)-binding and/or mineralization peptides, and the AgNP mineralization activity of each peptide was broadly evaluated. Among 10 peptides showing the highest AgNP-synthesis activity (TOP10), nine showed the presence of EE and E[X]E (E: glutamic acid, and X: any amino acid), whereas none of these motifs were found in the WORST25 (25 peptides showing the lowest AgNP synthesis activity) peptides. The size and morphology of the particles synthesized by TOP3 peptides were dependent on their sequences. These results suggested not only that array-based techniques are effective for the peptide screening of AgNP mineralization, but also that AgNP mineralization regulated by peptides has the potential for the synthesis of AgNPs, with controlled morphology in environmentally friendly conditions.

Biomimetic mineralization based on self-assembling peptides

“…Afterward, Tanaka et al employed an array comprising 200 spot synthesis-based peptides and broadly evaluated the Ag NP mineralization activity of each peptide. 139 The size and morphology of the peptide-mineralized Ag NPs depend on the sequence of the peptide.…”

Structure, Characteristics and Corrosion Behaviour of Gold Nanocoating Thin Film for Biomedical Applications

“…Tanaka et al 3 reviewed several novel properties that happen when the dimensions of solid material shrunk to the point of no return comprise tens to a few thousand atoms. Due to their specific electrical and optical properties, nanostructured deposition plays now a crucial role in several science and technology materials.…”

“…After a close review of the literary works 3,17 on the deposition of gold films, showed that the depositions of gold nanostructured materials on different alloys have received a little bit of attention. Furthermore, the study of the literature indicates that few works have been used Ni-Cr-Mo alloy substrate for depositions of gold thin films nanostructure to produce biocorrosion resistant materials for biological, medicinal, and dental applications.…”

Systematic Screening and Deep Analysis of CoPt Binding Peptides Leads to Enhanced CoPt Nanoparticles Using Designed Peptides

“…The experimental procedures are relatively simple and use inexpensive reagents and equipment, with a wide range of choices for library design; but most importantly, each SPOT can be individually designed and probed, so analysis of each sequence can be obtained for a deeper understanding and evaluation of the binding process. Previously, SPOT synthesis has been used for a wide variety of applications such as mapping of epitopes for antibody binding, protein-peptide recognition, enzyme specificity, and identifying peptides for cell adhesion, , and most recently, we have led the innovation of using the method to explore the interactions between peptides and inorganic materials. − …”