Arsenic exposure during prepuberty alters prostate maturation in pubescent rats

Aquino, Ariana Musa de; Salata, Giovanna Cassone; Pinho, Cristiane Figueiredo; Freitas, André Teves Aquino Gonçalves de; Périco, Larissa Lucena; Siervo, Gláucia Elóisa Munhoz de Lion; Mendes, Leonardo O.; Medeiros, Paloma da Cunha de; Justulin, Luís Antônio; Fernandes, Glaura Scantamburlo Alves; Perobelli, Juliana Elaine; Scarano, Wellerson Rodrigo

doi:10.1016/j.reprotox.2019.07.010

Cited by 10 publications

(4 citation statements)

References 61 publications

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“…Similarly, in vivo arsenic exposure induces biochemical and morphological changes in adult rat testis (de Araujo Ramos et al, 2017). Given the marked differences between the pre-and post-pubertal testis, it is important to investigate effects of sodium arsenite directly on the developing male reproductive tissues; with, in contrast, little information available: thirty-day administration of sodium arsenite to prepubertal rats induced dysfunction of normal prostate development (Aquino et al, 2019), spermatogenesis and epididymal structure (da Cunha de . Here, we have used an in vitro culture system of prepubertal mouse testis to assess the impact of arsenic on the testicular development.…”

Section: Discussionmentioning

confidence: 99%

“…Prepubertal testicular development is a highly sensitive stage and it is possible that toxicants such as arsenic may act differently on testis of young compared to adult males. Presently, only a few animal studies have examined the effects of arsenic on the reproductive axis of the prepubertal mammal: prepubertal rat prostate development has been found to be affected (Aquino et al, 2019), but that study provides little information about the testicular cell populations that could be damaged (i.e. somatic and/or germ cells); in one other study, prepubertal rats exposed to sodium arsenite (0.01 or 10 mg/l) for 30 days in the drinking water showed a significant disruption of normal testicular morphology (da Cunha de .…”

Section: Introductionmentioning

confidence: 99%

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In vitro administration of sodium arsenite in mouse prepubertal testis induces germ cell loss and apoptosis

Anwar

Qureshi

Spears

et al. 2020

Toxicology in Vitro

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro administration of sodium arsenite in mouse prepubertal testis induces germ cell loss and apoptosis

Anwar

Qureshi

Spears

et al. 2020

Toxicology in Vitro

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“…The relative volume of collagen was quantified in picrosirius-stained histological sections following the instructions of Image J software (National Institute of Health, United States - NIH). Two histological sections were analyzed per animal (5 animals/group), with an interval of 50 μm between each section, and 10 histological fields/section were photographed at 40x objective, 400X magnification , as described by Aquino et al (2019) .…”

Section: Methodsmentioning

confidence: 99%

Fractal analysis is a useful tool for evaluating prostate tissue remodeling caused by ethanol consumption and androgen therapy

Lima,

Oliveira,

Sousa

et al. 2023

Anim. Reprod.

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Alcohol has been widely consumed for centuries and is linked to the aggravation of diseases. Several studies have shown that excessive consumption of ethanol results in morphophysiological changes in the male reproductive system. One of the effects of ethanol is the decrease in testosterone concentration and hormonal therapies are an alternative to minimize the changes resulting from chronic alcoholism. Qualitative studies were commonly carried out to evaluate the male histopathological alterations resulting from ethanol consumption, being necessary quantitative and non-subjective techniques. This study analyzes the importance of fractal analysis as a useful tool to identify and quantify tissue remodeling in rats submitted to ethanol consumption and hormone therapy with testosterone. Prostate of animals submitted to chronic ethanol consumption showed tissue disorganization, which was confirmed by an increasing of fractal dimension. Regarding the prostatic stroma, collagen fractal dimension and quantification revealed lower values in animals that were only submitted to androgen therapy. Thus, we can conclude that the fractal analysis was a useful tool to quantify tissue changes caused by ethanol consumption and androgen therapy.

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“…Histological sections of five animals per groups were analyzed and photographed (10 histological fields/section, at 40x magnification), totaling 100 measurements per group. The system was programmed to only recognize red and estimate the collagen (stained red) percentage per histological field (AQUINO et al, 2019).…”

Section: Quantification Of the Area Occupied By Collagenmentioning

confidence: 99%

Assessment of prostate tissue remodeling in rats exposed to bisphenol A and the phytoestrogens genistein and indole-3-carbinol during the perinatal period

Bueno,

Vieira Neto,

Rodrigues

et al. 2024

Cienc. Rural

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Bisphenol A (BPA) is a compound known for its direct action on the prostate. Prostatic morphogenesis is a critical period when interference by any compound could permanently damage the organ. As such, the present study evaluated the morphological aspects resulting from gestational and lactational administration of BPA, indole-3-carbinol (I3C) and genistein (GEN) in prepubescent male rats. Pregnant Sprague Dawley females were allocated into 4 experimental groups and received the following: C: Control (no treatment); B: BPA (10 µg/Kg); BG: BPA+GEN (5 mg/Kg); BI: BPA+I3C (20 mg/Kg) from gestation day (GD) 17 to postnatal day (PND) 21. After euthanasia on PND22, the prostate was collected and processed. When administered alone, BPA reduced the stromal compartment when compared to group C (P = 0.039). This decline was reversed in the groups submitted to GEN (P = 0.019) or I3C (P = 0.017). The groups treated with BPA (P < 0.0001) and the phytoestrogens (P < 0.0001) exhibited decreased epithelial height in relation to the control group. These changes were observed in stereological and morphometric analyses, but not in fractal analysis (P = 0.569). The area occupied by collagen increased in groups treated with BPA (P < 0.0001) and phytoestrogens (P < 0.0001) in relation to controls, while collagen distribution values were higher in all the treated groups (P < 0.0001), according to fractal analysis. Thus, BPA induced prostrate stroma remodeling with no influence from the phytoestrogens, which may affect glandular development and cause histopathological changes in adulthood.

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Arsenic exposure during prepuberty alters prostate maturation in pubescent rats

Cited by 10 publications

References 61 publications

In vitro administration of sodium arsenite in mouse prepubertal testis induces germ cell loss and apoptosis

In vitro administration of sodium arsenite in mouse prepubertal testis induces germ cell loss and apoptosis

Fractal analysis is a useful tool for evaluating prostate tissue remodeling caused by ethanol consumption and androgen therapy

Assessment of prostate tissue remodeling in rats exposed to bisphenol A and the phytoestrogens genistein and indole-3-carbinol during the perinatal period

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