2016
DOI: 10.1177/1934578x1601100604
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Artemisinic Acid Serves as a Novel ORCA3 Inducer to Enhance Biosynthesis of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells

Abstract: To investigate the effect of artemisinic acid (AA) on improving the production of terpenoid indole alkaloids (TIAs) of Catharanthus roseus cambial meristematic cells (CMCs), feeding AA to C. roseus CMCs caused 2.35-fold and 2.51-fold increases in the production of vindoline and catharanthine, respectively, compared with those of the untreated CMCs. qRT-PCR experiments showed that AA resulted in a 1.36-8.52 fold increase in the transcript levels of several related genes, including octadecanoid-derivative respon… Show more

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Cited by 4 publications
(4 citation statements)
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“…The alkaloid extraction from 6-day-old suspensions of C. roseus CMCs was performed according to the methods of Wang [31]. Alkaloid extracts were dissolved in 1.0 mL of methanol and analyzed by HPLC-MS/MS according to the methods of Ferreres et al [32] with slight modifications.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The alkaloid extraction from 6-day-old suspensions of C. roseus CMCs was performed according to the methods of Wang [31]. Alkaloid extracts were dissolved in 1.0 mL of methanol and analyzed by HPLC-MS/MS according to the methods of Ferreres et al [32] with slight modifications.…”
Section: Methodsmentioning
confidence: 99%
“…Alkaloid extraction was performed according to the methods of Wang et al [31]. Alkaloid extracts were dissolved in 1.0 mL of methanol and analyzed by HPLC and ion-pair extraction-spectrophotometry as described by Yang et al [33].…”
Section: Methodsmentioning
confidence: 99%
“…Besides JA, other ORCA3 inducers are found recently. Feeding artemisinic acid to C. roseus meristematic cells resulted in the increase of the transcript level of ORCA3 , which indicated that artemisinic acid may be another way to induce ORCA3 [ 35 ]. Another study shows that inoculation of fungal endophytes in C. roseus could up-regulate the expression of ORCA3 and enhance the accumulation of vindoline [ 36 ].…”
Section: Transcriptional Regulation Of Tia Biosynthesismentioning
confidence: 99%
“…Overexpressing either ORCA2 or ORCA3 in C. roseus suspension cells or hairy roots could change the expression of several key genes in the TIAs biosynthetic pathway ( Van der Fits and Memelink, 2000 ; Pan et al, 2012 ; Li et al, 2013 ; Sun and Peebles, 2016 ). In addition, ORCA3 could be regulated by many inducers, such as jasmonic acid, artemisinic acid and fungal endophytes ( Peebles et al, 2009 ; Pandey et al, 2016 ; Wang et al, 2016 ). Another two AP2/ERF members, ORCA4 and ORCA5, were cloned recently.…”
Section: Introductionmentioning
confidence: 99%