Arterial delivery of genetically labelled skeletal myoblasts to the murine heart: Long-term survival and phenotypic modification of implanted myoblasts

Robinson, Shawn; Cho, Peter W.; Olson, Jean L.; Hruban, Ralph H.; Acker, Michael A.; Kessler, Paul D.

doi:10.1016/0963-6897(95)02016-0

Cited by 89 publications

(53 citation statements)

References 44 publications

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“…It can be performed at the same time as percutaneous coronary intervention (PCI). Studies have demonstrated the integration of the injected cells into myocardial tissue via the expression of cardiac gap-junction and connexin 43 proteins localized to some of these interfaces between implanted cells and cardiomyocytes after successful delivery of myoblasts in different animal models [63][64][65][66]. Clinical studies have also been carried out, and the results of these studies have demonstrated the feasibility, safety, and efficacy of this method [45][46][47].…”

Section: Intracoronary Cell Injectionmentioning

confidence: 97%

Stem cells and cardiovascular tissue repair: Mechanism, methods, and clinical applications

Zhang

Madonna

Shi

et al. 2006

Journal of Cardiothoracic-Renal Research

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Section: Intracoronary Cell Injectionmentioning

confidence: 97%

Stem cells and cardiovascular tissue repair: Mechanism, methods, and clinical applications

Zhang

Madonna

Shi

et al. 2006

Journal of Cardiothoracic-Renal Research

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“…Many treatments attempt to reduce some of the effects of the ischemic region but no treatment induces replacement or augmentation of the ischemic cardiac cells (Cleland et al 2001;Goldstein 2001;Miniati and Robbins 2002;Stevenson and Kormos 2001). Although gene therapy and injection of skeletal (Atkins et al 1999;Murry et al 1996;Robinson et al 1996;Zibaitis et al 1994) or cardiac cells (Jia et al 1997;Baldwin 1996;Matsushita et al 1999;Reinecke et al 1999;Scorsin et al 1997;Watanabe et al 1998) has been investigated, replacement of ischemic cells and improved cardiac function has not been reported. Another approach is to tissue engineer a three-dimensional (3D) construct of cardiac cells that could be grafted onto the ischemic region (Carrier et al 1999;Eschenhagen et al 2002;Li et al 2000;Shimizu et al 2002;Zimmermann et al 2000).…”

Section: Introductionmentioning

confidence: 94%

Cardiac cells implanted into a cylindrical, vascularized chamber in vivo: pressure generation and morphology

et al. 2008

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We have previously described a model to implant dissociated cells into a cylindrical, vascularized bed in vivo to promote the formation of functional cardiac muscle constructs. We now investigate the cellular organization and the ability of the constructs to generate intra-luminal pressure. Primary cardiac cells were isolated from hearts of 2-3 day old rats, suspended in fibrin gel and inserted into the lumen of silicone tubing. The silicone tubing was then implanted around the femoral vessels in the groin region of recipient animals. After 3 weeks, the constructs were harvested, placed in an in vitro bath and cannulated via the incorporated femoral artery with a pressure transducer for evaluation of intra-luminal pressure dynamics. Histological evaluation showed the presence of a concentric ring of cardiac cells surrounding the femoral vessels. There was also a significant amount of collagen present around cardiac cells. In addition, we observed a significant amount of neovascularization of the explanted constructs. Electron microscopy showed the presence of longitudinally aligned fibers with a large number of gap junctions. Upon electrical stimulation of a single pulse (7 V, 1.2 ms), the constructs generated an intra-luminal pressure of 1.19 +/- 0.45 mmHg (n = 6). In addition, we were able to electrically pace the constructs at frequencies of 0.5-5 Hz. A Starling behavior of the inverse relation between baseline pressure and twitch pressure was observed. Cardiac cells implanted for 3 weeks into the cylindrical vascularized bed formed a tissue construct that demonstrated many of the contractile properties and morphology expected of functioning cardiac tissues.

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“…Different cell sources are being evaluated and have been utilized to develop cell transplantation therapies: adult-derived stem cells skeletal myoblasts [147][148][149][150][151][152], bone-marrow-derived cells [153][154][155][156][157], and endothelial progenitor cells [158,159] or embryonic stem cells [160][161][162][163][164][165]. Various studies have demonstrated the differentiation of mouse and human embryonic stem (ES) cells into functional cardiomyocytes [166][167][168].…”

Section: Discussionmentioning

confidence: 99%

Getting to the Heart of Tissue Engineering

Khait

Hecker

Blan

et al. 2008

J. of Cardiovasc. Trans. Res.

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Cardiovascular disease affects 80 million people in the USA and is the leading cause of death. Significant limitations of current treatments necessitate the development of novel strategies. Cardiovascular tissue engineering is an emerging field focused on the development of biological substitutes to restore, maintain, or improve tissue function. In this article, we present an overview of trends in the field and scientific milestones achieved during the last decade. Various 3D bioengineered models of functional cardiovascular structures, including cell-based cardiac pumps, ventricles, patches, vessels, and valves, are described. We discuss critical technological hurdles that must be addressed for continued progress and an outlook for the future of cardiovascular tissue engineering.

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Arterial delivery of genetically labelled skeletal myoblasts to the murine heart: Long-term survival and phenotypic modification of implanted myoblasts

Cited by 89 publications

References 44 publications

Stem cells and cardiovascular tissue repair: Mechanism, methods, and clinical applications

Stem cells and cardiovascular tissue repair: Mechanism, methods, and clinical applications

Cardiac cells implanted into a cylindrical, vascularized chamber in vivo: pressure generation and morphology

Getting to the Heart of Tissue Engineering

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