Arthrographis kalrae soluble antigens present hemolytic and cytotoxic activities

Nagashima, Luciene Airy; Akagi, Claudia Yuri; Sano, Ayako; Silva, Paula Leonello Álvares e; Murata, Yoshiteru; Itano, Eiko Nakagawa

doi:10.1016/j.cimid.2014.09.002

Cited by 3 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given that heating only diminished the lytic effect of CFA, the hemolytic component must: (i) be heat resistant; (ii) have an active site that is not altered by changes in protein conformation; (ii) not be a protein; or (iv) have both thermo‐labile and thermo‐resistant components. Previous studies have shown certain fungi have more than one component with hemolytic activity, for example, A. kalrae and C. parapsilosis . In addition to non‐protein components of fungi, proteases may also be responsible for hemolysis by yeast cells .…”

Section: Discussionmentioning

confidence: 99%

“…Cell suspensions had greater than 95% viability. Live yeast cells (10 1 up to 10 7 ), heat‐killed yeast cells (autoclaved for 15 min at 121°C), Arthrographis kalrae (positive control, causes strong hemolysis) and PBS (negative control) were incubated with 1% human erythrocytes suspensions (v/v, total volume of 200 μL) in V‐bottom plates (3 hr, 37°C).…”

Section: Methodsmentioning

confidence: 99%

“…After incubation, plates were centrifuged (155 g , 10 min, 4°C) and the supernatants collected and analyzed at 550 nm (Multiskan EX; Labsystems, Helsinki, Finland) . Experiments were performed in duplicate.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Paracoccidioides restrepiensis B339 (PS3) and P. lutzii LDR2 yeast cells and soluble components display in vitro hemolytic and hemagglutinating activities on human erythrocytes

Cezar-dos-Santos

Lenhard-Vidal

Assolini

et al. 2018

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

Paracoccidioidomycosis (PCM) is a systemic mycosis caused by thermodimorfic fungi of Paracoccidioides species complex. Several pathogenic fungi produce hemagglutinins and hemolysins, which are virulence factors involved in adhesion of pathogens to host tissues or cells and in destruction of erythrocytes. The present research investigated hemolytic and hemagglutinating activities of yeast cells and soluble components from P. restrepiensis (PS3; former P. brasiliensis B339) and P. lutzii (LDR2). Different concentrations of live and heat-killed yeast cells and soluble components from cell free antigen preparation (CFA) (native or heated - 56 and 100 °C, 30 min) were mixed with 1% human erythrocyte suspension. Yeast cells from both species caused hemolysis, but P. lutzii LDR2 was more hemolytic than P. restrepiensis B339, while the opposite phenomena occurred with soluble components in most conditions. Live or heat-killed yeast cells of both fungi agglutinated erythrocytes, but only heated soluble components from P. restrepiensis B339 showed hemagglutinating activity. In conclusion, yeast cells of P. restrepiensis B339 and P. lutzii LDR2 produce hemolysins and hemagglutinins, which most likely are more restricted to yeast cells in P. lutzii LDR2 and are more released in soluble form byP. restrepiensis B339, requiring further study.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%