Arthropod venoms: A vast arsenal of insecticidal neuropeptides

Schwartz, Elisabeth Ferroni; Mourão, Caroline Barbosa Farias; Moreira, Karla; Camargos, Thalita Soares; Mortari, Márcia Renata

doi:10.1002/bip.22100

Cited by 42 publications

(28 citation statements)

References 290 publications

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“…Arthropod predators such as spiders, scorpions, and centipedes mainly use their venom to catch insect prey, and consequently their venoms contain many insecticidal peptides (Schwartz et al, 2012;King and Hardy, 2013;Smith et al, 2013). Due to hundreds of millions of years of evolutionary selection pressure, these insecticidal toxins have developed remarkable selectivity and potency for their molecular targets (Smith et al, 2013).…”

Section: Insecticidal Venom Peptides From Arthropod Predatorsmentioning

confidence: 99%

“…In the venoms of spiders alone, which are among the world's most successful insect predators, it is estimated that millions of insecticidal toxins are yet to be discovered (Schwartz et al, 2012;King and Hardy, 2013;Smith et al, 2013). Venom peptides from these predators can act with high affinity on ion channels, membrane receptors, and neurotransmitter transporters in the nervous system of invertebrate prey (Schwartz et al, 2012;Smith et al, 2013).…”

Section: Insecticidal Venom Peptides From Arthropod Predatorsmentioning

confidence: 99%

“…There are ~1500 extant species with conserved morphology (Dehesa-Davila et al, 1994;Chowell et al, 2006). Scorpions can survive in extreme environments; they remain hidden during the day and emerge at night to feed on small insects (Schwartz et al, 2012). As for spiders, scorpion venom is a complex mixture of small molecules such as serotonin and histamine, protease inhibitors, SS-rich neurotoxic peptides, enzymes such as hyaluronidase and phospholipase, and mucopolysaccharides (Goudet et al, 2002;Fernandes-Pedrosa et al, 2013).…”

Section: Insecticidal Peptides From Scorpion Venommentioning

confidence: 99%

“…These insects are classified in two groups based on their life history: social and solitary. The great majority of both social and solitary wasps are predators, including many agricultural pests (Schwartz et al, 2012). The venoms of social hymenopterans evolved to be used as defensive tools to protect colonies of these insects from attacks by predators.…”

Section: Insecticidal Peptides From Hymenopteran Venomsmentioning

confidence: 99%

“…Among the high molecular weight compounds there are many types of allergens (reviewed in (Schwartz et al, 2012)), such as antigen 5 (Hoffman, 1993;Cascone et al, 1995;Pirpignani et al, 2002), hyaluronidases (Kolarich et al, 2005), and phospholipases (Soldatova et al, 1993;Abe et al, 2000;Costa and Palma, 2000).…”

Section: Insecticidal Peptides From Hymenopteran Venomsmentioning

confidence: 99%

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Engineering insect-resistant plants by transgenic expression of an insecticidal spider-venom peptide

Alam¹

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The insecticidal spider-venom peptide ω-hexatoxin-Hv1a (Hv1a) from the Australian Blue Mountains funnel-web spider Hadronyche versuta is one of the most potent insect-specific neurotoxins isolated to date. Hv1a blocks voltage-gated calcium channels in the insect central nervous system, a mechanism quite distinct from existing chemical insecticides. It induces a slow-onset paralysis that precedes death in a taxonomically wide range of insects. Hv1a's broad spectrum of target insects, novel mode of action, and absence of toxicity to vertebrates makes the Hv1a gene an attractive tool for generating insectresistant transgenic crops.The oral activity of Hv1a can be enhanced by coupling it to the plant lectin Galanthus nivalis agglutinin (GNA) or with the minor capsid protein of pea enation mosaic virus (CP).Recombinant fusions of Hv1a with GNA were produced using the Pichia pastoris expression system to study the intrinsic insecticidal activity of Hv1a-GNA and GNA-Hv1a fusion proteins. By using injection bioassays with houseflies, we found that the intrinsic insecticidal activity of Hv1a was maintained when it was fused to GNA. Moreover, feeding bioassays with diamondback moth larvae revealed that fusion of Hv1a to GNA, in either orientation, enhances its oral insecticidal activity.In order to generate transgenic plants expressing Hv1a alone or fused to GNA or CP, transformation vectors were constructed by ligating synthesised genes in the pAOV binary vector with the constitutive Cauliflower Mosaic Virus 35S promoter (35S) or the phloem tissue-specific Arabidopsis thaliana SUCROSE TRANSPORTER 2 (SUC2) promoter.Homozygous transgenic Arabidopsis were generated using the floral dip method of Agrobacterium-mediated plant transformation and subsequent herbicide selection. PCR of genomic DNA and western blotting were used to confirm integration of the transgenes and protein expression in the transgenic Arabidopsis respectively. Initial experiments revealed a very high level of mortality of Helicoverpa armigera larvae on wild-type plants due to the presence of endogenous glucosinolates, which masked the insecticidal effects of the transgenes Thus, a new set of transgenic plants was generated using an Arabidopsis cyp79B2 cyp79B myb28 myb29 quadruple mutant that lacks endogenous glucosinolates.Bioassays revealed that H. armigera larvae had a lower level of survival and retarded growth when fed on leaves of transgenic Arabidopsis expressing Hv1a toxins under 35S promoter control compared with those fed on gluc-null control plants. Moreover, larval mortality was higher for plants expressing Hv1a/GNA fusions than those expressing Hv1a or GNA alone. The highest larval mortality, lowest larval weight gain, and lowest level of leaf damage were observed for larvae fed on plants expressing GNA-Hv1a. Mortality was extremely high (~90%) for larvae fed on GNA-Hv1a plants for 15 days. The resistance to cotton bollworms conferred by expression of GNA-Hv1a in transgenic Arabidopsis highlights the potential of Hv1a transgenes as an ...

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Section: Insecticidal Venom Peptides From Arthropod Predatorsmentioning

confidence: 99%

Section: Insecticidal Venom Peptides From Arthropod Predatorsmentioning

confidence: 99%

Section: Insecticidal Peptides From Scorpion Venommentioning

confidence: 99%

Section: Insecticidal Peptides From Hymenopteran Venomsmentioning

confidence: 99%

Section: Insecticidal Peptides From Hymenopteran Venomsmentioning

confidence: 99%

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Engineering insect-resistant plants by transgenic expression of an insecticidal spider-venom peptide

Alam¹

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Peptides from agribusiness to human health

Franco¹,

Santos

2012

Biopolymers

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This article presents the results of a study of infant diet at two Iron Age sites on the island of Öland, Sweden. The cemetery at Bjärby contained a large number of subadults who had survived the earliest years of life, whereas most individuals at Triberga had died by 6 months of age. To investigate whether differences in infant feeding could explain the different mortality rates, the carbon, nitrogen, and sulfur stable isotope ratios of bone and tooth dentin collagen from the two sites were analyzed. Twenty‐two samples from Triberga and 102 from Bjärby yielded data that could be included in the carbon and nitrogen analysis. Twelve samples from Triberga and 42 from Bjärby were included in the sulfur analysis. The results for carbon (δ13C: Triberga X = –18.8, s.d. = 1.1; Bjärby X = –19.8, s.d. = 0.4), nitrogen (δ15N: Triberga X = 12.9, s.d. = 1.5; Bjärby X = 13.4, s.d. = 1.4), and sulfur (δ34S: Triberga X = 8.1, s.d. = 1.1; Bjärby X = 5.8, s.d. = 1.3) suggest that diet was broadly similar at both sites and based on terrestrial resources. At Bjärby, females and high‐status individuals consumed higher‐trophic level protein than other males from early childhood onward. There was some indication that the contribution of marine resources to the diet may also have differed between the sexes at Triberga. No consistent differences in breast milk intake were observed between the two sites, but there was substantial variation at each. This variation may reflect an influence of gender and social status on infant feeding decisions. Am J Phys Anthropol 149:217–230, 2012. © 2012 Wiley Periodicals, Inc.

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Origins, Technological Advancement, and Applications of Peptidomics

Schrader

2024

Methods in Molecular Biology

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Arthropod venoms: A vast arsenal of insecticidal neuropeptides

Cited by 42 publications

References 290 publications

Engineering insect-resistant plants by transgenic expression of an insecticidal spider-venom peptide

Engineering insect-resistant plants by transgenic expression of an insecticidal spider-venom peptide

Peptides from agribusiness to human health

Origins, Technological Advancement, and Applications of Peptidomics

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