Artificial diet is fruitful pre-storage nutrition for long-term cold storage of laboratory-reared Harmonia axyridis (Pallas) adults

Sun, Yuan-Xing; Hao, Yanan; Liu, Changzhong; Wang, Sen-Shan

doi:10.1016/j.biocontrol.2019.104075

Cited by 7 publications

(16 citation statements)

References 43 publications

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“…Chilling injury during storage was an important factor causing mortality 36 . In addition, other indirect effects including accumulation of toxic metabolites and elimination of energy reserves have also been suggested as probable reasons for death 1,3 . Here, with prolonged storage, the weight loss gradually increased and, especially, the glycogen and trehalose contents sharply decreased even at 30 DIS.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we still focused on the effect of cold storage on indoor-reared H. axyridis adults, and the adopted storage techniques were similar to those used in our previous studies with some modi cations 1 .…”

Section: Introductionmentioning

confidence: 99%

“…Brie y, β-carotene-amended arti cial diet was supplemented as pre-storage nutrition, and the rearing period was prolonged to 10 days due to the deduction that rearing on arti cial diet for longer periods might result in higher post-storage survivals 1,33 . After that, the adults were stored at 6°C for different periods (30,60,90, or 120 days), and their post-storage tness as well as offspring's reproductive capabilities were measured to evaluate the lethal and sublethal effects.…”

Section: Introductionmentioning

confidence: 99%

“…Long term cold storage of laboratory-reared natural enemy is very important for developing feasible biological control programs 1 , especially for those relying on large number releasing of natural enemies 2 .…”

Section: Introductionmentioning

confidence: 99%

“…However, H. axyridis is a "chill intolerant" predator 20 , and the logistics of low-temperature storage has been shown to be one of the most important limitations in using it in biological control programs 21,22 . Abundant studies have been done to improve the cold storage techniques, but most of them only focused on the eld collected pre-overwintering individuals 1 . For example, the quality of pre-storage diet has been con rmed to have notable effect on the post-storage tness of H. axyridis, and injured wine grape berries or sugar showed to be more optimal than water in decreasing mortality of overwintering beetles 23 .…”

Section: Introductionmentioning

confidence: 99%

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Lethal and Sublethal Effects of Long-term Cold Storage on Indoor-reared Harmonia Axyridis Adults

Sun

Hao

et al. 2021

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Indoor-reared natural enemy with high quality after long-term cold storage is crucial for sustainable pest management. However, besides survivals, the sublethal effects were not widely been reported. In this study, Harmonia axyridis (Pallas), an important biological control agent in Asia, was reared with β-carotene-amended artificial diet (Ha-Car) before storing at 6°C. After 30, 60, 90 and 120 days of storage, a series of biological parameters were measured to evaluate the fitness of H. axyridis. We found that: (1) Survivals significantly decreased with prolonged storage, and more Ha-Car individuals survived at day 120 compared to Ha-CK (control); (2) The contents of glycogen and trehalose dramatically decreased following storage, and the weight losses gradually increased; (3) The average egg production and hatch rates within 15 days were not significantly different among treatments Ha-Car and Ha-CK following long-term storage (90 and 120 days), while the daily hatch rates gradually decreased from relatively high to zero at day 14 and 15; (4) The number of micropyles deposited on eggs also gradually decreased along with oviposition period. After re-mating with a new non-stored partner, the egg viability gradually increased again, while low egg viability was still detected in F1 generation. Moreover, reduced number of micropyles were detected on their eggs. In summary, Ha-Car can be cold stored for about 120 days with relatively high survivals and fecundity, but long-term storage produced remarkable intra- and trans-generational negative effects on fertility. Even though, the cold-stored H. axyridis had great potential being used in biological control program with inevitable promiscuity with field individuals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%