Artificial diet rearing system for the silkworm Bombyx mori (Lepidoptera: Bombycidae): effect of vitamin C deprivation on larval growth and cocoon production

Cappellozza, L.; Cappellozza, Silvia; Saviane, Alessio; Sbrenna, G.

doi:10.1303/aez.2005.405

Cited by 64 publications

(49 citation statements)

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“…The larvae were fed with mulberry leaves (strain 7532) or artificial diet (polyhybrid strain) [47] and reared at 25 ± 0.5°C under a 12L:12D photoperiod and 70% relative humidity. After animals had ecdysed to the last larval stage (fifth instar), they were staged and synchronized according to Kiguchi and Agui [48].…”

Section: Experimental Animalsmentioning

confidence: 99%

Autophagy precedes apoptosis during the remodeling of silkworm larval midgut

et al. 2011

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Although several features of apoptosis and autophagy have been reported in the larval organs of Lepidoptera during metamorphosis, solid experimental evidence for autophagy is still lacking. Moreover, the role of the two processes and the nature of their relationship are still cryptic. In this study, we perform a cellular, biochemical and molecular analysis of the degeneration process that occurs in the larval midgut of Bombyx mori during larval-adult transformation, with the aim to analyze autophagy and apoptosis in cells that die under physiological conditions. We demonstrate that larval midgut degradation is due to the concerted action of the two mechanisms, which occur at different times and have different functions. Autophagy is activated from the wandering stage and reaches a high level of activity during the spinning and prepupal stages, as demonstrated by specific autophagic markers. Our data show that the process of autophagy can recycle molecules from the degenerating cells and supply nutrients to the animal during the non-feeding period. Apoptosis intervenes later. In fact, although genes encoding caspases are transcribed at the end of the larval period, the activity of these proteases is not appreciable until the second day of spinning and apoptotic features are observable from prepupal phase. The abundance of apoptotic features during the pupal phase, when the majority of the cells die, indicates that apoptosis is actually responsible for cell death and for the disappearance of larval midgut cells.

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Section: Experimental Animalsmentioning

confidence: 99%

Autophagy precedes apoptosis during the remodeling of silkworm larval midgut

et al. 2011

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“…The silkworm were reared in controlled conditions [11] and the larvae were fed ''ad libitum'' with artificial diet also obtained by Sericulture Specialized Unit of Padua of the Experiment Institute for Agrarian Zoology [11] until 2 nd day of 5 th instar.…”

Section: Modified Feed Experimentsmentioning

confidence: 99%

Naturally functionalized silk as useful material for photonic applications

Cavallini

Toffanin

Chieco

et al. 2015

Composites Part B: Engineering

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“…Maintenance, Pyridalyl treatment, and dissection of insects B. mori eggs and larvae [four-way polyhybrid strain (126 Â 57) Â (70 Â 79)] were maintained under a photoperiod of 16 h dark: 8 h light at 25 C and relative humidity 70e80%. Larvae were kept in plastic boxes and reared on an artificial mulberry leaf diet (Cappellozza et al, 2005).…”

Section: Protein Extractionmentioning

confidence: 99%