Artificial enzyme mimics cascade catalysis for signal amplification and transduction in food quality determination: An overview of fundamentals and recent advances

Li, Yuechun; Bao, Qinyuan; Wang, Ziqi; Huang, Yijia; Zhang, Daohong; Shen, Yizhong; Cheng, Jie; Wang, Jianlong

doi:10.1016/j.ccr.2024.215689

Cited by 13 publications

(3 citation statements)

References 117 publications

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“…14 These nanozymebased cascade reactions are versatile, allowing the detection of a broad range of substances such as acetylcholine, cholesterol, and glucose, by integrating specific natural enzymes with nanozymes. 15 Furthermore, employing multiple nanozymes with different enzymatic activities can create a biomimetic cascade sensing platform that enhances detection sensitivity, specificity, and operational simplicity. 15,16 Building on the potential of nanozyme-based systems, we present a sensitive, selective, and visible colorimetric strategy for NQO1 detection.…”

Section: ■ Introductionmentioning

confidence: 99%

“…15 Furthermore, employing multiple nanozymes with different enzymatic activities can create a biomimetic cascade sensing platform that enhances detection sensitivity, specificity, and operational simplicity. 15,16 Building on the potential of nanozyme-based systems, we present a sensitive, selective, and visible colorimetric strategy for NQO1 detection. This approach utilizes a substrate competitive reaction paired with a biomimetic cascade reaction (Scheme 1).…”

Section: ■ Introductionmentioning

confidence: 99%

“…In response to these challenges, nanozymes-nanomaterials with intrinsic enzyme-like activities-emerge as effective substitutes for natural enzymes . These nanozyme-based cascade reactions are versatile, allowing the detection of a broad range of substances such as acetylcholine, cholesterol, and glucose, by integrating specific natural enzymes with nanozymes . Furthermore, employing multiple nanozymes with different enzymatic activities can create a biomimetic cascade sensing platform that enhances detection sensitivity, specificity, and operational simplicity. , …”

Section: Introductionmentioning

confidence: 99%

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Innovative Colorimetric NQO1 Detection Strategy via Substrate Competitive and Biomimetic Cascade Reactions with a Highly Active NADH Oxidase Mimic

Fang,

Liu,

Song

et al. 2024

Anal. Chem.

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NAD(P)H: quinone oxidoreductase-1 (NQO1) plays critical roles in antioxidation and abnormally overexpresses in tumors. Developing a fast and sensitive method of monitoring NQO1 will greatly promote cancer diagnosis in clinical practice. This study introduces a transformative colorimetric detection strategy for NQO1, harnessing an innovative competitive substrate mechanism between NQO1 and a new NADH oxidase (NOX) mimic, cobalt−nitrogen-doped carbon nanozyme (CoNC). This method ingeniously exploits the differential consumption of NADH in the presence of NQO1 to modulate the generation of H 2 O 2 from CoNC catalysis, which is then quantified through a secondary, peroxidase-mimetic cascade reaction involving Prussian blue (PB) nanoparticles. This dual-stage reaction framework not only enhances the sensitivity of NQO1 detection, achieving a limit of detection as low as 0.67 μg mL −1 , but also enables the differentiation between cancerous and noncancerous cells by their enzymatic activity profiles. Moreover, CoNC exhibits exceptional catalytic efficiency, with a specific activity reaching 5.2 U mg −1 , significantly outperforming existing NOX mimics. Beyond mere detection, CoNC serves a dual role, acting as both a robust mimic of cytochrome c reductase (Cyt c) and a cornerstone for enzymatic regeneration, thereby broadening the scope of its biological applications. This study not only marks a significant step forward in the bioanalytical application of nanozymes but also sets the stage for their expanded use in clinical diagnostics and therapeutic monitoring.

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Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Innovative Colorimetric NQO1 Detection Strategy via Substrate Competitive and Biomimetic Cascade Reactions with a Highly Active NADH Oxidase Mimic

Fang,

Liu,

Song

et al. 2024

Anal. Chem.

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Aggregation‐based analytical chemistry in point‐of‐care nanosensors

Li,

Cui,

Huang

et al. 2024

Aggregate

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It is crucial to realize the point‐of‐care (POC) testing of harmful analytes, capable of saving limited agricultural resources, assisting environmental remediation, ensuring food safety, and enabling early disease diagnosis. Compared with other conventional POC sensing strategies, aggregation‐based analytical chemistry facilitates the practical‐oriented development of POC nanosensors by altering the aggregation status of nanoprobes through the action of multiple aggregation‐induced “forces” originating from the targets. Herein, we have proceeded with a comprehensive review focusing on the aggregation‐based analytical chemistry in POC nanosensors, covering aggregation‐induced “forces”, aggregation‐induced signal transductions, aggregation‐induced POC nanosensing strategies, and their applications in biomolecular monitoring, food safety analysis, and environmental monitoring. Finally, challenges existing in practical applications have been further proposed to improve their sensing applications, and we expect our review can speed up the development of cost‐effective, readily deployable, and time‐efficient nanosensors through aggregation‐based analytical chemistry.

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Chemiluminescence detection of kanamycin by DNA aptamer regulating peroxidase-like activity of Co3O4 nanoparticles

Zhang,

Li,

Xia

et al. 2024

ANAL. SCI.

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Artificial enzyme mimics cascade catalysis for signal amplification and transduction in food quality determination: An overview of fundamentals and recent advances

Cited by 13 publications

References 117 publications

Innovative Colorimetric NQO1 Detection Strategy via Substrate Competitive and Biomimetic Cascade Reactions with a Highly Active NADH Oxidase Mimic

Innovative Colorimetric NQO1 Detection Strategy via Substrate Competitive and Biomimetic Cascade Reactions with a Highly Active NADH Oxidase Mimic

Aggregation‐based analytical chemistry in point‐of‐care nanosensors

Chemiluminescence detection of kanamycin by DNA aptamer regulating peroxidase-like activity of Co3O4 nanoparticles

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