Artificial-epitope mapping for CK-MB assay

Tai, Dar-Fu; Ho, Y P; Wu, Cheng-Hsin; Lin, Tzu‐Chieh; Lu, Kuo-Hao; Lin, Kun-Shian

doi:10.1039/c0an00919a

Cited by 16 publications

(17 citation statements)

References 30 publications

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“…A number of papers report assays based upon competitive ligand binding studies . The high sensitivities of assays using either radiolabeled or non‐isotopically labeled studies (fluorescence and chemiluminescence) afford access to events taking place at very‐low concentration regimes that are not generally accessible with chromatographic methods.…”

Section: Applicationsmentioning

confidence: 99%

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

2014

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SummaryHerein we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004 to 2011. In total, 3779 references to the original papers, reviews, edited volumes and monographs from this period are included, along with recently identified uncited materials from prior to 2004 which were omitted in the first instalment of this series covering the years 1930 to 2003. 1 In the presentation of the assembled references, a section presenting reviews and monographs covering the area is followed by sections describing fundamental aspects of molecular imprinting including the development of novel polymer formats. Thereafter, literature describing efforts to apply these polymeric materials to a range of application areas is presented. Current trends and areas of rapid development are discussed.2

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Section: Applicationsmentioning

confidence: 99%

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

2014

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“…The HIV PR 85–94 peptide templated HEMIPs were prepared as a co-polymer on a QCM chip using acrylic acid, acrylamide, N -benzylamide and EBAA ( N , N’ -ethylene bisacrylamide), in line with our previous studies [ 22 , 23 , 24 , 25 ]. First, an amino acid cross-linker ( N -Acr- l -Cys-NHBn) 2 was used as a thiol-ene monomer, which enables simple functionalization with binding groups for the polymer’s immobilization of gold chips and an initiator for UV-irradiated photopolymerization.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, an epitope template-based system is simpler and safer for detecting HIV PR and screening its inhibitors. Our studies have shown that an MIP-coated QCM sensor using epitope imprinting significantly strengthens the detection of protective antigens and their segments [ 22 , 23 ]. These results also suggest that functionalized epitope cavities on a QCM surface can effectively catch an oriental antigen.…”

Section: Introductionmentioning

confidence: 99%

Sensing HIV Protease and Its Inhibitor Using “Helical Epitope”—Imprinted Polymers

Chou

Lin

et al. 2020

Sensors

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A helical epitope-peptide (lle85-Gly94) was selected from the α-helix structure of the HIV protease (PR) as the template, which represents an intricate interplay between structure conformation and dimerization. The peptide template was mixed with water, trifluoroethanol (TFE), and acetonitrile (ACN) at a certain ratio to enlarge the helical conformation in the solution for the fabrication of helical epitope-mediated molecularly imprinted polymers (HEMIPs) on a quartz crystal microbalance (QCM) chip. The template molecules were then removed under equilibrium batch rebinding conditions involving 5% acetic acid/water. The resulting HEMIPs chip exhibited a high affinity toward template peptide HIV PR85–94, His-tagged HIV PR, and HIV PR, with dissociation constants (Kd) as 160, 43.3, and 78.5 pM, respectively. The detection limit of the developed HIV PR85–94 QCM sensor is 0.1 ng/mL. The HEMIPs chip exhibited a high affinity and selectivity to bind HIV PR and subsequently to an inhibitor of HIV PR (nelfinavir). The HIV PR binding site was properly oriented on the HEMIPs-chip to develop a HIV PR/HEMIPs chip, which can effectively bind nelfinavir to establish a sandwich assay. The nelfinavir then attached to the HIV PR/HEMIPs chip, which can be easily removed involving 0.8% acetic acid/water. Therefore, HIV PR/HEMIPs chip can be useful to screen for other HIV PR inhibitors. This technique may improve drug targeting for HIV therapy and also strengthen investigations into other virus assays.

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“…Tai et al developed a MIP-QCM assay for the detection of creatine kinases [52]. Linear epitope sequences were selected based on an artificial-epitope mapping strategy.…”

Section: Mip-based Quartz Crystal Microbalance Bioassaysmentioning

confidence: 99%

Molecularly Imprinted Polymers as Tools for Bioassays and Biotransformation

Liu

Huang

et al. 2015

Molecularly Imprinted Polymers in Biotechnology

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In the past five years, significant progress has been made in preparation of various molecularly imprinted polymer (MIP)-based materials for applications in bioassays and biotransformation. This chapter reviews the important advances in these two fields. The first part mainly focuses on the development of various MIP-based bioassays that convert the rebinding of template to the imprinted cavities into measurable luminescent signals, including fluorescence, phosphorescence, Raman scattering, diffraction, and the like. In addition, MIP-based bioassays that are measured by surface plasmon resonance or quartz crystal microbalance are also discussed. In the following part, representative biotransformation reactions that make use of MIPs are summarized. In the last part of this chapter, some remaining challenges are briefly discussed for further development of the two fields.

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Artificial-epitope mapping for CK-MB assay

Cited by 16 publications

References 30 publications

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

Sensing HIV Protease and Its Inhibitor Using “Helical Epitope”—Imprinted Polymers

Molecularly Imprinted Polymers as Tools for Bioassays and Biotransformation

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