Background and Objectives: Recent advances in intraoperative navigation systems have improved the accuracy of pedicle screw placement in spine surgery. However, many hospitals have limited access to these advanced technologies due to resource constraints. In such settings, postoperative computed tomography (CT) evaluation remains crucial for assessing screw placement and related potential complications. Metal artifacts in CT scans often compromise the diagnostic accuracy. This study aimed to develop and validate three-dimensional (3-D) reconstruction software to enhance screw localization accuracy and facilitate its practical clinical application. Materials and Methods: This study included two phases: 3-D software development utilizing specific threshold values of Hounsfield units for titanium screws followed by internal validation. For validation, fifty pedicle screws were inserted into porcine lumbar vertebrae with random violation (superior, inferior, medial, or lateral). Three fellowship-trained surgeons evaluated screw positions using both conventional CT bone window settings and the developed software. Additional clinical validation involving 386 pedicle screws from cervical to lumbar spine was performed by two surgeons. Results: The software demonstrated significantly higher specificity (83% vs. 63%) and positive predictive value (96% vs. 91%) compared to conventional CT bone window settings, while maintaining 100% sensitivity and negative predictive value. Interobserver reliability was excellent for both methods (0.961 for bone window vs. 0.990 for software). In clinical validation, the software showed superior intraobserver (0.83 vs. 0.74) and interobserver reliability (0.855 vs. 0.513) compared to picture archiving and communication system (PACS) workstation evaluation. Conclusions: The developed software provides improved accuracy and reliability in pedicle screw position evaluation through distinct screw outline visualization and metal artifact reduction. Its equipment-independent nature and cost-effectiveness make it particularly valuable for clinical implementation.