Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection

Nasr-Esfahani, Mohammad Hossein; Razavi, Shahnaz; Javdan, Z; Tavalaee, Marziyeh

doi:10.1016/j.fertnstert.2007.10.047

Cited by 84 publications

(52 citation statements)

References 50 publications

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“…Injected-activated oocytes in the globozoospermic group and injected oocytes in the fertile group were examined for evidence of fertilization (determined by the presence of two pronuclei) at 16-18 h post-ICSI. The fertilization rate was calculated based on the number of fertilized oocytes per total number of oocytes injected, multiplied by 100 (Nasr-Esfahani et al, 2008, 2010b.…”

Section: Intra-cytoplasmic Sperm Injection and Aoamentioning

confidence: 99%

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

Tavalaee

Nasr-Esfahani

2016

Andrology

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SUMMARYSperm-mediated oocyte activation critically depends upon appropriate expression and assembly of sperm-borne oocyte-activating factors (SOAFs) during spermiogenesis. Several factors have been considered as candidate for SOAFs over the recent years. However, little is known about the expression profile of these candidates and their potential contribution to the clinical outcomes of intra-cytoplasmic sperm injection (ICSI), particularly in globozoospermia. This study investigated the expression profile of PLCf, PAWP, and TR-KIT and clinical outcomes of ICSI in 12 men with total globozoospermia and compared with 12 fertile individuals. Levels of PLCf, PAWP, and TR-KIT mRNA in the spermatozoa of fertile men were significantly higher than the corresponding values of the globozoospermic subjects. Interestingly, at protein level, expressions of these factors in the cases assessed were low in globozoospermic individuals. Fertilization rates following artificial oocyte activation (AOA) in the majority of globozoospermic couples were higher than the expected 30% cut-off value reported for individuals with failed or low fertilization rate. Clinical outcomes of ICSI-AOA were dependent on inter-individual variation in globozoospermic couples. None of the SOAFs assessed could provide a greater prediction value with respect to fertilization rate in globozoospermic couples which underwent ICSI-AOA. High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCf, PAWP, and TR-KIT were low in globozoospermic individuals, and ICSI combined with artificial oocyte activation could mimic physiological calcium changes which occur during fertilization.

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Section: Intra-cytoplasmic Sperm Injection and Aoamentioning

confidence: 99%

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

Tavalaee

Nasr-Esfahani

2016

Andrology

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“…Recently, Nasr-Esfahani et al (37) reported that AOA with ionomycin can improve fertilization and cleavage rates in teratozoospermic patients; however, to date, there have been few studies using AOA in ICSI cycles with sperm from patients who have different types of azoospermia. We evaluated the effect of AOA with the calcium ionophore A23187 on ICSI cycles using sperm surgically retrieved from AO or NOA patients.…”

mentioning

confidence: 99%

Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa

Borges

Braga

Bonetti

et al. 2009

Fertility and Sterility

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“…In their study, Nasr-Esfahani et al [30] applied artificial oocyte activation to a large number of patients with no adverse effects on the health of the infants to date. Furthermore, chromosomal analysis using FISH has shown a normal chromosomal status of those embryos derived through artificial oocyte activation [31].…”

Section: Discussionmentioning

confidence: 99%

Case report: live birth following ICSI with non-vital frozen-thawed testicular sperm and oocyte activation with calcium ionophore

Stecher

Bach

Neyer

et al. 2011

J Assist Reprod Genet

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Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection

Cited by 84 publications

References 50 publications

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa

Case report: live birth following ICSI with non-vital frozen-thawed testicular sperm and oocyte activation with calcium ionophore

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