Artificial oocyte activation to improve reproductive outcomes in women with previous fertilization failure: a systematic review and meta-analysis of RCTs

Sfontouris, Ioannis A.; Nastri, C.O.; Lima, M. L. S.; Tahmasbpourmarzouni, Eisa; Raine‐Fenning, Nick; Martins, Wellington P.

doi:10.1093/humrep/dev136

Cited by 65 publications

(60 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Calcium signalling is crucial for oocyte activation and further embryonic development (Ducibella, Schultz, & Ozil, ; Kline & Kline, ; Marangos & Carroll, ; Ozil, Banrezes, Toth, Pan, & Schultz, ). An increase in fertilisation and cleavage‐stage embryo rates with the use of assisted oocyte activation protocols involving the use of Ca 2+ ‐ionophores has also been reported (Ebner et al., ; Heindryckx et al., ; Nikiforaki et al., ; Sfontouris et al., ; Vanden Meerschaut, Nikiforaki, Heindryckx, & De Sutter, ).…”

Section: Discussionmentioning

confidence: 86%

Human sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation-reduction potential

Roychoudhury

Rosas²,

Agarwal

et al. 2018

Andrologia

View full text Add to dashboard Cite

Polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) are routinely used in handling spermatozoa for intracytoplasmic sperm injection (ICSI). As there are still concerns about possible adverse effects on the embryo, this study investigated sperm handling in a mouse ICSI model to (i) evaluate oocyte activation after injection of spermatozoa selected for rotational or linear motion in PVP; (ii) assess the effect of sperm selection in PVP, HA and medium on oocyte activation; (iii) examine the effects of PVP and HA on parthenogenetic oocyte activation and embryo development; and (iv) assess the oxidation-reduction potential (ORP) of spermatozoa exposed to PVP, HA or medium. Oocyte activation was higher when spermatozoa exhibited rotational motion rather than linear motion (79% vs. 52%; p = .05). There was no difference in oocyte activation and embryo development after parthenogenetic oocyte activation after sperm injection using PVP, HA or medium-incubated spermatozoa. PVP-selected spermatozoa exhibited lower (p < .0001) ORP levels than using HA. Thus, results indicate that the sperm handling method and the type of medium used impact ICSI outcomes. Overall, sperm incubation in PVP, HA and medium yields similar outcomes with regard to oocyte activation and embryo development. However, PVP provides more antioxidative protection than HA and should therefore be preferred for sperm manipulation.

show abstract

Section: Discussionmentioning

confidence: 86%

Human sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation-reduction potential

Roychoudhury

Rosas²,

Agarwal

et al. 2018

Andrologia

View full text Add to dashboard Cite

show abstract

“…The systematic review and meta-analysis of RCTs that compared ICSI-AOA and conventional ICSI first established that there is insufficient evidence available from RCTs to judge the efficacy and safety of ICSI-AOA for couples with previous fertilization failure [89]. A total of 14 articles were assessed and 9 included in meta-analysis.…”

Section: Success Rates Of Aoamentioning

confidence: 99%

Oocyte Activation Failure: Physiological and Clinical Aspects

Hojnik¹,

Kovačič²

2019

Embryology - Theory and Practice

View full text Add to dashboard Cite

Despite successful treatment of infertility with assisted reproductive technology (ART), total fertilization failure (TFF) after in vitro fertilization (IVF) and even after intracytoplasmic sperm injection (ICSI) still occurs. In the current chapter, the incidence and etiology of TFF after ICSI are described. The literature on physiology of oocyte activation, electrical properties of gametes' membranes, and ion currents is reviewed. Calcium oscillations play an essential role in fertilization, and calcium ions act as secondary messengers in different metabolic pathways and cellular processes during oocyte activation. The contribution of oocyte-and spermrelated causes of fertilization failure is discussed. Many studies on the physiology of fertilization in mammals have shown that oocyte activation is triggered by the sperm factor. Methods for artificial oocyte activation (AOA) try to bypass fertilization failure by influencing physiological processes that are crucial for successful fertilization. Activation can be induced with the use of electrical, mechanical, or chemical stimuli that elevate intracellular concentrations of calcium ions. Different AOA methods and their success and safety are presented.

show abstract

“…Others have proposed electrical current-induced [44–46] or specific mechanical manipulation approaches [47]. A recent meta-analysis of randomized controlled trials concluded that currently there is not sufficient data to support either methodology in cases of fertilization failure or “rescue ICSI” [48]. Obviously, none of these techniques present the physiological Ca 2+ oscillations.…”

Section: Alternative Sperm Factor Post-acrosomal Ww-domain Binding Prmentioning

confidence: 99%

“This is where it all started” – the pivotal role of PLCζ within the sophisticated process of mammalian reproduction: a systemic review

Gat

Orvieto

2017

Basic Clin. Androl.

View full text Add to dashboard Cite

Mammalian reproduction is one of the most complex and fascinating biological phenomenon, which aims to transfer maternal and paternal genetic material to the next generation. At the end of oogenesis and spermatogenesis, both haploid gametes contain a single set of chromosomes ready to form the zygote, the first cell of the newly developing individual. The mature oocyte and spermatozoa remain in a quiescent state, during which the oocyte is characterized by nuclear and cytoplasmic arrest, while the spermatozoa necessitates further maturation within the epididymis and female reproductive track prior to egg fertilization. Either in vivo or in vitro, the sperm initiates a series of irreversible biochemical and physiological modifications in the oocyte. The earliest detected signal after fertilization is cytosolic Ca2+ oscillations, a prerequisite step for embryo development. These oscillations trigger the release of the oocyte from the second meiosis arrest towards embryogenesis, also known as “oocyte activation”. Phospholipase C zeta (PLCζ) is a unique sperm-soluble protein responsible for triggering the InsP3/Ca2+ pathway within the oocyte, leading to Ca2+ oscillations and consequently to embryo development. The specific structure of PLCζ (compared to other PLCs) enables its specialized activity via the preserved X and Y catalytic domains, as well as distinct features such as rapid onset, high sensitivity to Ca2+ and cession of oscillations upon zygote formation. The emerging discoveries of PLCζ have stimulated studies focusing on the possible clinical applications of this protein in male infertility evaluation and management during IVF/ICSI. Fertilization failure is attributed to lack of oocyte second meiosis resumption, suggesting that ICSI failure may be related to impaired PLCζ activity. Microinjection of recombinant human PLCζ to human oocytes after ICSI fertilization failure may trigger Ca2+ oscillations and achieve successful fertilization, offering new hope for couples traditionally referred to sperm donation. However, more studies are still required prior to the routine implementation of this approach in the clinic. Directions for future studies are discussed.

show abstract

Artificial oocyte activation to improve reproductive outcomes in women with previous fertilization failure: a systematic review and meta-analysis of RCTs

Abstract: PROSPERO CRD42014007445.

Cited by 65 publications

References 56 publications

Human sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation-reduction potential

Human sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation-reduction potential

Oocyte Activation Failure: Physiological and Clinical Aspects

“This is where it all started” – the pivotal role of PLCζ within the sophisticated process of mammalian reproduction: a systemic review

Contact Info

Product

Resources

About