Artificial Oocyte: Development and Potential Application

Oqani, Reza K.; So, Seongjun; Lee, Yeonmi; Ko, Jung Jae; Kang, Eunju

doi:10.3390/cells11071135

Cited by 4 publications

(2 citation statements)

References 169 publications

(198 reference statements)

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“…This methodology opens the possibility for application in fertility preservation because it does not require embryonic gonads. Taken together, we can conclude that germ cells could be generated from mouse somatic cells without transfection of transcription factors [ 272 , 275 ]. Human oogonia also could be generated from PSCs in culture [ 276 , 277 ].…”

Section: Ovarian Aging Intervention Strategymentioning

confidence: 99%

Ovarian aging: mechanisms and intervention strategies

Zhu

Liu

2022

Medical Review

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Ovarian reserve is essential for fertility and influences healthy aging in women. Advanced maternal age correlates with the progressive loss of both the quantity and quality of oocytes. The molecular mechanisms and various contributing factors underlying ovarian aging have been uncovered. In this review, we highlight some of critical factors that impact oocyte quantity and quality during aging. Germ cell and follicle reserve at birth determines reproductive lifespan and timing the menopause in female mammals. Accelerated diminishing ovarian reserve leads to premature ovarian aging or insufficiency. Poor oocyte quality with increasing age could result from chromosomal cohesion deterioration and misaligned chromosomes, telomere shortening, DNA damage and associated genetic mutations, oxidative stress, mitochondrial dysfunction and epigenetic alteration. We also discuss the intervention strategies to delay ovarian aging. Both the efficacy of senotherapies by antioxidants against reproductive aging and mitochondrial therapy are discussed. Functional oocytes and ovarioids could be rejuvenated from pluripotent stem cells or somatic cells. We propose directions for future interventions. As couples increasingly begin delaying parenthood in life worldwide, understanding the molecular mechanisms during female reproductive aging and potential intervention strategies could benefit women in making earlier choices about their reproductive health.

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Section: Ovarian Aging Intervention Strategymentioning

confidence: 99%

Ovarian aging: mechanisms and intervention strategies

Zhu

Liu

2022

Medical Review

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“…Artificial oocytes are required for infertility treatment of people around the world and the number increasing every year (Oqani et al, 2022). In recent years, assisted reproductive technologies have been widely developed (Pavlović et al, 2024;Desy and Marre 2024).…”

mentioning

confidence: 99%

Do the Mammalian Artificial Oocytes Repair Reproductive Dysfunctions in Mammalian Species?: A Review

Mohammed,

AlGherair,

Al-Suwaiegh

et al. 2024

IJAR

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Artificial oocytes were used of different mammalian species for repairing reproductive dysfunctions. Artificial oocytes are those created in vitro using enucleated germinal vesicle, metaphase I and metaphase II oocytes followed by embryonic cells, somatic cells, pluripotent stem cells and germ cells nuclear transfer. Artificial oocytes are under development, they have the potential to revolutionize reproduction in mammalian species. The potential applications of artificial oocytes include 1) treatment of infertility as premature ovarian failure, ovarian cancer, or other conditions that damage the ovaries, 2) preservation of fertility, 3) creating cloned and transgenic animals. While artificial oocytes have the potential to revolutionize reproductive medicine, there are still a number of challenges that need to be addressed before they can be effectively and safely used in humans including the adapting efficient and safe enucleation techniques. Furthermore, cellular reprogramming is the biggest obstacle for creating artificial oocytes in addition to activation procedures to ensure that artificial oocytes are genetically and epigenetically normal and producing healthy offspring. Several studies are designed to overcome these challenges and there is significant progresses being made. Offspring are obtained in several animals species whereas developed blastocysts were obtained in humans. This review is developed and implemented to discuss the progress in creation of “artificial oocytes” in addition to the factors affecting the developmental competence of reconstructed oocytes.

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