Artificial reproduction of neotropical fish: Extrusion or natural spawning?

Reynalte‐Tataje, David Augusto; Lopes, Carolina Antonieta; Ávila-Simas, Sunshine de; Garcia, Juan Ramón Esquivel; Zaniboni‐Filho, Evoy

doi:10.4236/ns.2013.57a001

Cited by 9 publications

(15 citation statements)

References 7 publications

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“…Semaprochilodus insignis, Prochilodus argenteus, Prochilodus costatus, Prochilodus lineatus) (Godinho et al, 2017;Smith et al, 2018;Borie et al, 2019) and anostomids (e.g. Megaleporinus muyscorum, Leporinus microcephalus) (Reynalte-Tataje et al, 2013;Ramirez et al, 2017). Both of these families are in the same monophyletic clade (Anostomoidea), which is thought to be the sister group of Serrasalmidae (Calcagnotto et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Origin and evolution of sound production in Serrasalmidae

Mélotte

Raick

Vigouroux

et al. 2019

Biological Journal of the Linnean Society

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Among piranhas, sound production is known in carnivorous species, whereas herbivorous species were thought to be mute. Given that these carnivorous sonic species have a complex sonic apparatus, we hypothesize that intermediate forms could be found in other serrasalmid species. The results highlight the evolutionary transition from a simple sound-producing mechanism without specialized sonic structures to a sonic mechanism involving large, fast-contracting sonic muscles. Hypaxial muscles in basal herbivores primarily serve locomotion, but some fibres cause sound production during swimming accelerations, meaning that these muscles have gained a dual function. Sound production therefore seems to have been acquired through exaptation, i.e. the development of a new function (sound production) in existing structures initially shaped for a different purpose (locomotion). In more derived species (Catoprion and Pygopristis), some fibres are distinguishable from typical hypaxial muscles and insert directly on the swimbladder. At this stage, the primary function (locomotion) is lost in favour of the secondary function (sound production). In the last stage, the muscles and insertion sites are larger and the innervation involves more spinal nerves, improving calling abilities. In serrasalmids, the evolution of acoustic communication is characterized initially by exaptation followed by adaptive evolution.

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Section: Discussionmentioning

confidence: 99%

Origin and evolution of sound production in Serrasalmidae

Mélotte

Raick

Vigouroux

et al. 2019

Biological Journal of the Linnean Society

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“…Acredita-se que isso tenha ocorrido devido a técnica empregada, a fertilização a seco. Um estudo comparando a fertilização a seco e a desova natural mostrou que, em quatro das cinco espécies utilizadas, a taxa de fertilização foi superior quando se utilizou a desova natural [14]. Uma possível explicação para esse fato é a manipulação dos gametas (particularmente ovócitos).…”

Section: Discussionunclassified

Fertilizing Capacity of the Cryopreserved Sperm of Prochilodus brevis

Nunes

Oliveira-Araújo

Lopes

et al. 2019

Acta Scientiae. Vet.

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Background: Seminal cryopreservation is a technique that optimizes aquacultural production, as it requires less breeding and enables reproduction outside of the breeding season. This technique also helps to preserve species, thus reducing the pressure on the natural stocks. Several studies have sought to develop freezing protocols that result in semen of a good quality. However, some studies do not evaluate the ability of frozen semen to produce viable larvae. Therefore, the aim of this study was to verify the fertilizing capacity of the frozen semen of Prochilodus brevis.Materials, Methods & Results: Semen from twenty adult males of the Brazilian bocachico was collected and evaluated to establish the total motility, curvilinear velocity, straight linear velocity, average path velocity, membrane integrity, pH, and concentration. Six pools were formed, each of which was diluted in a freezing medium containing 5% glucose with 10% dimethyl sulfoxide (DMSO) or 5% glucose with 10% methyl glycol (MG). The samples were loaded into 0.25 mL French straws, frozen in a dry shipper, and stored in a liquid nitrogen canister. The semen was then thawed and evaluated to establish the total motility, curvilinear velocity, straight linear velocity, average path velocity, and membrane integrity. For the fertilization test, four females were used. The oocytes from each female were divided into three batches and fertilized with either fresh or cryopreserved semen. The rates of fertilization, hatching, and larval survival were then measured. Data were expressed as the mean ± standard deviation and analyzed using SAS (2002). The frozen semen with glucose + DMSO was significantly higher (P < 0.001) than the frozen semen with glucose + MG, in all seminal quality parameters evaluated (63.95 ± 15.88% and 25.36 ± 3.53% for the motility, 36.38 ± 7.02 μm.s-1 and 20.45 ± 2.84 μm.s-1 for the curvilinear velocity, 19.26 ± 2.74 μm.s-1 and 3.03 ± 1.40 μm.s-1 for the straight linear velocity, 25.70 ± 6.51 μm.s-1 and 6.90 ± 1.12 μm.s-1 for the average path velocity, and 63.58 ± 6.95% and 35.58 ± 11.26% for the membrane integrity, respectively). MG were very close to zero and not statistically significant. Regarding these same parameters, there were no significant differences (P > 0.05) when the fresh semen was compared to the cryopreserved semen with glucose + DMSO (36.25 ± 2.5% and 29.16 ± 5.64% for the fertilization rate, 38.56 ± 11.23% and 29.33 ± 11.75% for the hatching rate, and 11.59 ± 5.16% and 7.63 ± 5.46% for the larval survival rate, respectively).Discussion: This is the first study of the artificial fertilization of Prochilodus brevis using cryopreserved semen. Seminal quality parameters are important for predicting the success of the cryopreservation technique, however, in vivo tests are essential to confirm such success. Thus, obtaining larvae is a major step towards the standardization of a cryopreservation protocol for a particular species. It is known that cryopreservation reduces the seminal quality but is a necessary process for the conservation of male gametes in the long term and, as shown in this study, good results can be obtained. In this study, the best results were obtained with the inclusion of DMSO in the freezing medium. This effect can be attributed to DMSO having a very low molecular weight, which decreases the formation of ice crystals. Considering the results obtained, we concluded that it is feasible to obtain larvae of the Brazilian bocachico using frozen semen in a 5% glucose solution with 10% DMSO.

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“…Inadequate management of broodstocks and lack of monitoring of both natural populations and specimens released in the rivers through stocking programs may trigger future loss of genetic diversity, generating homogenization (Satake and Araki, 2012). Consequently, correct stocking management -considering the biological characteristics of each species-is crucial for the production of quality fingerlings (Reynalte-Tataje et al, 2013) and this, naturally, includes the maintenance of genetic variability (Lopera-Barrero et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil

Lopera-Barrero

Santos

Castro

et al. 2019

Rev Colom Cienc Pecua

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Background: Piracanjuba (Brycon orbignyanus) is a fish species highly affected by anthropogenic actions such as overfishing, water pollution, and hydroelectric developments. This species is currently considered in danger of extinction. Objective: To analyze the genetic diversity of a natural population (NP) and two captive broodstocks (SA and SB) of B. orbignyanus. Methods: Samples of caudal fins (NP: 24, SA: 30, and SB: 30) were collected. DNA was extracted and amplified for six RAPD primers and four microsatellite loci. Results: Sixty polymorphic fragments and 17 microsatellite alleles were detected. High intrapopulation heterozygosity (NP: 0.692, SA: 0.724, and SB: 0.686) was observed. Thirty-eight fragments and six alleles were shared among NP, SA, and SB. The FIS and Shannon’s Index of diversity revealed a lack of inbreeding within groups. AMOVA analyses and FST indicated very high (NP vs SA and SB) and small (SA vs SB) genetic differentiation, confirmed by genetic distance and identity, number of migrants and a dendrogram, which revealed the formation of two genetic groups. Conclusions: The two marker types showed similar variability. The groups have adequate genetic variability, with high differentiation between NP and SA–SB, and similarity between broodstocks.Key words: aquaculture, Brycon orbignyanus, genetic conservation, microsatellites, RAPD. ResumenAntecedentes: Piracanjuba (Brycon orbignyanus) es una especie de pez fuertemente impactada por acciones antrópicas como sobrepesca, contaminación del agua y proyectos hidroeléctricos. Esta especie está considerada en peligro de extinción. Objetivo: Analizar la diversidad genética de una población natural (NP) y de dos lotes de reproductores (SA y SB) de B. orbignyanus en cautiverio. Métodos: Se colectaron 84 muestras de aleta caudal (NP: 24, SA: 30 y SB: 30). El ADN fue extraído y amplificado para seis cebadores RAPD y cuatro loci microsatélites. Resultados: Se obtuvieron 60 fragmentos polimórficos y 17 alelos microsatélites. Se observó alta heterocigosidad intra-poblacional (NP: 0,692; SA: 0,724 y SB: 0,686). Treinta y ocho fragmentos y seis alelos fueron compartidos entre NP, SA y SB. Los valores de FIS e índice de Shannon mostraron ausencia de endogamia entre los grupos. Los análisis de ANOVA y FST indicaron alta (NP vs SA y SB) y pequeña (SA vs SB) diferenciación genética; resultados confirmados por la distancia e identidad genética, número de migrantes y dendograma, evidenciando la formación de dos grupos genéticos. Conclusiones: Los grupos poseen adecuada variabilidad genética, con alta diferenciación entre NP vs SA-SB y similitud entre los lotes de reproductores.Palabras clave: acuicultura, Brycon orbignyanus, conservación genética, microsatélites, RAPD. Resumo Antecedentes: Piracanjuba (Brycon orbignyanus) é uma espécie peixe fortemente impactada por ações antrópicas como sobrepesca, poluição e construção de hidrelétricas. Atualmente, essa espécie engloba a lista de peixes que correm perigo de extinção. Objetivo: Analisar a diversidade genética de uma população natural (NP) e de dois estoques de reprodutores em cativeiro (SA e SB) de B. orbignyanus. Métodos: Foram coletadas amostras de nadadeira caudal de 84 indivíduos (NP: 24, SA: 30 e SB: 30). O DNA foi extraido e amplificado para seis primers RAPD e quatro loci microssatélites. Resultados: Foram obtidos 60 fragmentos polimórficos e 17 alelos microssatélites. Foi observada uma alta heterozigosidade intra-populacional (NP: 0,692; SA: 0,724 e SB: 0,686). Trinta e oito fragmentos e seis alelos foram compartilhados entre NP, SA e SB. Os valores de FIS e índice de Shannon demonstraram ausência de endogamia entre os grupos. As análises de AMOVA e FST indicaram alta (NP vs SA e SB) e pequena (SA vs SB) diferenciação genética, resultados confirmados pela distância e identidade genética, número de migrantes e dendrograma, que evidenciaram a formação de dois grupamentos genéticos. Conclusões: Os grupos possuem adequada variabilidade genética, com alta diferenciação entre NP e SA-SB e similaridade entre os estoques de reprodutores.Palabras-chaves: aquicultura, Brycon orbignyanus, conservação genética, microssatélites, RAPD.

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Artificial reproduction of neotropical fish: Extrusion or natural spawning?

Cited by 9 publications

References 7 publications

Origin and evolution of sound production in Serrasalmidae

Origin and evolution of sound production in Serrasalmidae

Fertilizing Capacity of the Cryopreserved Sperm of Prochilodus brevis

Genetic diversity of Piracanjuba populations in fish stocking programs in the Tietê River, Brazil

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