Artificially altered gravity elicits cell homeostasis imbalance in planarian worms, and cerium oxide nanoparticles counteract this effect

Salvetti, Alessandra; Degl’Innocenti, Andrea; Gambino, Gaetana; Loon, Jack J. W. A. van; Ippolito, Chiara; Ghelardoni, Sandra; Ghigo, Éric; Leoncino, Luca; Prato, Mirko; Rossi, Leonardo; Ciofani, Gianni

doi:10.1002/jbm.a.37215

Cited by 5 publications

(3 citation statements)

References 76 publications

(132 reference statements)

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“…We considered as blastema the unpigmented region below the wound epithelium; blastemal margin was manually highlighted by the operator in blind. As described in Salvetti et al [58], in order to normalize differences in blastema area that might depend on the use of animals of different sizes, we chose the ratio between blastema area and total body area as the most appropriate parameter to evaluate effects on tissue regeneration. 5FU was purchased from Sigma (F6627, Sigma-Aldrich) and diluted in DMSO to obtain a 400 mM stock solution.…”

Section: Methodsmentioning

confidence: 99%

Cell quiescence in planarian stem cells, interplay between p53 and nutritional stimuli

et al. 2022

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Cell quiescence appeared early in evolution as an adaptive response to adverse conditions (i.e. nutrient depletion). In metazoans, quiescence has been involved in additional processes like tissue homeostasis, which is made possible by the presence of adult stem cells (ASCs). Cell cycle control machinery is a common hub for quiescence entrance, and evidence indicates a role for p53 in establishing the quiescent state of undamaged cells. Mechanisms responsible for waking up quiescent cells remain elusive, and nutritional stimulus, as a legacy of its original role, still appears to be a player in quiescence exit. Planarians, rich in ASCs, represent a suitable system in which we characterized a quiescent population of ASCs, the dorsal midline cord (DMC) cells, exhibiting unique transcriptional features and maintained quiescent by p53 and awakened upon feeding. The function of DMC cells is puzzling and we speculate that DMC cells, despite retaining ancient properties, might represent a functional drift in which quiescence has been recruited to provide evolutionary advantages.

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Section: Methodsmentioning

confidence: 99%

Cell quiescence in planarian stem cells, interplay between p53 and nutritional stimuli

et al. 2022

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“…This experiment utilized a gravity simulator and discovered that CNPs were able to mitigate the growth delays for the worms that is usually associated under reduced gravity environment. This is most likely due to the oxidative stress that this environment results in and the NPs's role in mitigating this effect (Salvetti et al, 2021). While this experiment does not consider space radiation, the role of oxidative stress in the space environment should be considered in developing therapeutic solutions.…”

Section: Ceria Nps As Radioprotectantsmentioning

confidence: 99%

Nanotechnology enabled radioprotectants to reduce space radiation‐induced reactive oxidative species

Babu

Pawar

Mittal

et al. 2023

WIREs Nanomed Nanobiotechnol

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Interest in space exploration has seen substantial growth following recent launch and operation of modern space technologies. In particular, the possibility of travel beyond low earth orbit is seeing sustained support. However, future deep space travel requires addressing health concerns for crews under continuous, longer‐term exposure to adverse environmental conditions. Among these challenges, radiation‐induced health issues are a major concern. Their potential to induce chronic illness is further potentiated by the microgravity environment. While investigations into the physiological effects of space radiation are still under investigation, studies on model ionizing radiation conditions, in earth and micro‐gravity conditions, can provide needed insight into relevant processes. Substantial formation of high, sustained reactive oxygen species (ROS) evolution during radiation exposure is a clear threat to physiological health of space travelers, producing indirect damage to various cell structures and requiring therapeutic address. Radioprotection toward the skeletal system components is essential to astronaut health, due to the high radio‐absorption cross‐section of bone mineral and local hematopoiesis. Nanotechnology can potentially function as radioprotectant and radiomitigating agents toward ROS and direct radiation damage. Nanoparticle compositions such as gold, silver, platinum, carbon‐based materials, silica, transition metal dichalcogenides, and ceria have all shown potential as viable radioprotectants to mitigate space radiation effects with nanoceria further showing the ability to protect genetic material from oxidative damage in several studies. As research into space radiation‐induced health problems develops, this review intends to provide insights into the nanomaterial design to ameliorate pathological effects from ionizing radiation exposure.This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Nanotechnology Approaches to Biology > Cells at the Nanoscale Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease

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“…Salvetti et-al. [3] conducted experiments to study the effects of artificially altered gravities on cells and microorganisms. For this purpose, they utilized a random positioning machine to simulate microgravity Indonesian J Elec Eng & Comp Sci ISSN: 2502-4752  and a large-diameter centrifuge to simulate hypergravity.…”

Section: Introductionmentioning

confidence: 99%

Development of a payload for monitoring biological samples in microgravity and hypergravity conditions

Canto-Vivanco,

Ramos-Cosi,

Romero-Alva

et al. 2024

IJEECS

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This research aims to address the need for monitoring the behavior of organic and inorganic materials in hypergravity conditions. To fulfill this objective, a container with specific features was designed. The container has a box with a lid, measuring 10×10×10 cm, conforming to the 1U volume of the CubeSat standard. It includes four cylindrical spaces to accommodate the sample wells. The container was 3D printed using polylactic acid (PLA) wire. For the electronic components, four ESP32-CAM modules were utilized, with two programmed to capture and upload photos to the cloud, and the other two programmed to capture and store photos on a micro SD memory card. Additionally, four light emitting diodes (LEDs) were incorporated to illuminate the well spaces. The total weight of the container is 450 grams, and it has a maximum wireless upload distance of 10 meters to the cloud. The storage capacity of the SD memory card determines the number of images that can be saved.

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Artificially altered gravity elicits cell homeostasis imbalance in planarian worms, and cerium oxide nanoparticles counteract this effect

Cited by 5 publications

References 76 publications

Cell quiescence in planarian stem cells, interplay between p53 and nutritional stimuli

Cell quiescence in planarian stem cells, interplay between p53 and nutritional stimuli

Nanotechnology enabled radioprotectants to reduce space radiation‐induced reactive oxidative species

Development of a payload for monitoring biological samples in microgravity and hypergravity conditions

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