Arylated gold nanoparticles have no effect on the adipogenic differentiation of MG-63 cells nor regulate any key signaling pathway during the differentiation

Abstract

Objective MG-63 cells that have osteoblastic and adipogenic differentiation potential were evaluated for internalization, and adipogenic differentiation in the presence and absence of the covalently functionalized aryl gold nanoparticles (AuNPs-C6H4-4-COOH). Results Inductively coupled plasma, flow cytometry and confocal microscopy analyses confirmed that gold nanoparticles were easily internalized by MG-63 cells. The MG-63 cells were differentiated into adipocytes without gold-aryl nanoparticles and with the gold-aryl nanoparticles at 5 µM concentration in both induction and maintenance media. The lipid content assay and the relative expressions of PPAR-γ, ADR1, GLUT1 and GLUT4 genes showed no significant variation with and without the gold nanoparticles treatment. Differential phosphorylation levels of 43 kinases phosphorylation sites were evaluated using the human phospho-kinase array to assess the effect of the gold nanoparticles on the signaling pathways during the differentiation. No kinase phosphorylation site was differentially phosphorylated with two or more folds after the nanoparticles treatment after the first day as well as at the end of MG-63 cells differentiation. The gold-aryl nanoparticles do not affect MG-63 cells differentiation into adipocytes neither do they affect any key signaling pathway. These properties make these gold nanoparticles suitable for future drug delivery and medical applications.

Assessing the toxicity of one-step-synthesized PEG-coated gold nanoparticles: in vitro and in vivo studies

“…The labeling of MG-63 cells with AuNPs in previous studies ( 34 , 35 ) reported no significant effects on the cellular cycle or key signaling pathways after labeling with up to 40µM AuNPs, consistent with our results. Steckiewicz et al ( 36 ) reported that high cell viability exceeding 95% was observed in MG-63 cell labeling with concentrations of up to 5µg/mL of spherical AuNPs, whereas our study demonstrated 96.18% viability even at concentrations of up to 100µg/mL, representing a mere 3.82% compromise in viability.…”

Gold nanoparticles: promising biomaterials for osteogenic/adipogenic regulation in bone repair

“…For example, arylated AuNPs did not mediate the adipogenic differentiation of MG-63 cells or regulate any key signalling pathway during their differentiation. 79 In summary, ligands with diverse charge and chemical characteristic can exert a decisive influence on their osteogenic property.…”

Gold Nanoparticle-Mediated Gene Therapy

“…Additionally, AuNPs could potentially aid in the study of genetic diseases. As was already indicated, substantial research on the absorption of important signaling pathways using MG-63 cells with gold–aryl NPs has been conducted by Abdulwahab et al [ 127 ]. They suggested that these particles may be suitable for drug delivery and tracing studies during development.…”