Ascaridoidea: a simple DNA assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds

Kijewska, Agnieszka; Rokicki, Jerzy; Sitko, J.; Węgrzyn, Grzegorz

doi:10.1016/s0014-4894(02)00031-0

Cited by 49 publications

(34 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Results of RFLP were shown during a high resolution electrophoresis on 4% agarose gel with ethidium bromide added. An image was then compared with templates (D'Amelio et al 2000, Kijewska et al 2002;La Rosa et al 2006 ).…”

Section: Methodsmentioning

confidence: 99%

Anisakids of seals found on the southern coast of Baltic Sea

Skrzypczak

Rokicki

Pawliczka

et al. 2014

Acta Parasitologica

Self Cite

View full text Add to dashboard Cite

In the present study 5 grey seals (Halichoerus grypus), 3 common seals (Phoca vitulina) and 1 ringed seal (Pusa hispida) bycaught or stranded on the Polish Baltic Sea coast in years 2000-2006 were investigated for the infestation of parasitic anisakid nematodes. 749 of anisakids were found. The most common were: Contracaecum osculatum (59.3%) and Pseudoterranova decipiens (31.0%). There were also small numbers of Anisakis simplex (0.8%). After performing RFLP three sibling species were found. C. osculatum was identified as C. osculatum C, P decipiens was identified as P. decipiens sensu stricto and A. simplex -A. simplex sensu stricto. Nematodes found in seals were mostly in L4 and adult life stage -both of them were equal with some minor variations among the specimens. Sex ratio was also equal, but there was slight excess of males in some cases. There was a minority of L3 larvae belonging to A. simplex species (0.8%).

show abstract

Section: Methodsmentioning

confidence: 99%

Anisakids of seals found on the southern coast of Baltic Sea

Skrzypczak

Rokicki

Pawliczka

et al. 2014

Acta Parasitologica

Self Cite

View full text Add to dashboard Cite

show abstract

“…Molecular anisakid nematode identification started with allozyme analyses including restriction fragment length polymorphism techniques (PCR-RFLPs of ITS-DNA, e.g. D'Amelio et al 2000;Kijewska et al 2002;Pontes et al 2005). The next approach was direct sequencing of rDNA, including the highly variable internal transcribed spacers ITS1-2 and the conserved 5.8S rDNA region; direct sequencing of the 28S (LSU rDNA) and complete internal transcribed spacer (ITS-1, 5.8S, ITS-2) rDNA (e.g.…”

Section: Genetic Identificationmentioning

confidence: 99%

Anisakid Nematode (Ascaridoidea) Life Cycles and Distribution: Increasing Zoonotic Potential in the Time of Climate Change?

Klimpel

Palm

2011

Progress in Parasitology

View full text Add to dashboard Cite

show abstract

“…The pUC Mix Marker (0.5 µg µl −1 ; Marker 8, Fermentas) was used as a marker. Product sizes were compared to rDNA ITS1-5.8S-ITS2 digestion patterns keys (D'Amelio et al 2000, Kijewska et al 2002.…”

Section: Restriction Fragment Length Polymorphismmentioning

confidence: 99%

Anisakidae in beluga whales Delphinapterus leucas from Hudson Bay and Hudson Strait

Najda

Simard²,

Osewska³

et al. 2015

Dis. Aquat. Org.

View full text Add to dashboard Cite

A total of 190 nematodes was isolated from the stomachs of 13 beluga whales Delphinapterus leucas from the Arctic part of Hudson Bay and Hudson Strait. Infection intensity ranged from 1 to 57 specimens and prevalence was 84.62%. Morphological examination of the nematodes revealed the presence of 3 species: Pseudoterranova decipiens sensu lato, Contracaecum osculatum s.l., and Anisakis simplex s.l. Molecular analysis by PCR-based restriction fragment length polymorphism (PCR-RFLP) resulted in the identification of 4 species: Pseudoterranova bulbosa, Contracaecum osculatum A and C, and Anisakis simplex sensu stricto. The nematodes were present in 3 developmental stages: L3 (159 specimens), L4 (16 larvae), and adults (15 worms: 11 males and 4 females).KEY WORDS: Anisakis · Contracaecum · Pseudoterranova · Canada · Cetacean · Parasite · ZoonosisResale or republication not permitted without written consent of the publisher

show abstract

Ascaridoidea: a simple DNA assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds

Cited by 49 publications

References 15 publications

Anisakids of seals found on the southern coast of Baltic Sea

Anisakids of seals found on the southern coast of Baltic Sea

Anisakid Nematode (Ascaridoidea) Life Cycles and Distribution: Increasing Zoonotic Potential in the Time of Climate Change?

Anisakidae in beluga whales Delphinapterus leucas from Hudson Bay and Hudson Strait

Contact Info

Product

Resources

About