Ascorbate metabolism in relation to oxidative stress

Smirnoff, Nicholas; Pallanca, Jane E.

doi:10.1042/bst0240472

Cited by 176 publications

(96 citation statements)

References 20 publications

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“…The GPx active site contains selenium in the form of the selenocysteine residue (Flohe et a\.,1973;Stadtrnan, 1991). There has been no report of a selenium dependent glutathione peroxidase activity in plants and HzOz is degraded by ascorbate peroxidase (Bunkelmann and Trelease, 1996;Smirnoff, 1996). (Fita and Rossman, 1985 (Klotz and Loewen, 2003 (Murshudov eÍ al., 1982), Proteus ntirabilis (PMC) (Gouet et al, 1995), E'çcherichia coli (HPII) (Bravo et a\.,1995 and, human erythrocytes (HEC) (Ko el al',1999;Puntam et al, lggg), Pseudomonas syrengae (CatF) (Carpena et a\.,2001 and (Murshudov et a|.,1996) (Kono and Irridovich, 1983), but now are more commonly called Mn-containing catalases (Allgood and Pery, 1986), with the terms non-heme (Nicholls, 2001) (Hillar et a\.,1999).…”

Section: The Oxygen Moleculementioning

confidence: 99%

Comparative study of catalase-peroxidases (KatGs)

Singh¹,

Wiseman²,

Deemagarn³

et al. 2008

Archives of Biochemistry and Biophysics

107

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Section: The Oxygen Moleculementioning

confidence: 99%

Comparative study of catalase-peroxidases (KatGs)

Singh¹,

Wiseman²,

Deemagarn³

et al. 2008

Archives of Biochemistry and Biophysics

107

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“…There is, however, evidence that plants can ' acclimate ' to O $ . Prior exposure to the pollutant (Held, Mooney & Gorham, 1991 ;Kangasja$ rvi et al, 1994) or other oxidative stresses (Polle & Rennenberg, 1993 ;Smirnoff & Pallanca, 1996) commonly leads to an induction of cellular defence and repair systems as well as compensatory changes in growth and morphology (Walmsley, Ashmore & Bell, 1980 ;Krupa & Manning, 1988 ;Held et al, 1991 ;Kasana, 1991 ;Younglove et al, 1994) which can limit the effects of prolonged exposure to O $ . Few studies have focused on whole-plant acclimation responses and attempted to separate shifts in O $ resistance with plant age from the those induced by prior exposure to the pollutant (i.e. '…”

Section: mentioning

confidence: 99%

Influence of plant age on ozone resistance in Plantago major

Lyons¹,

Barnes²

1998

New Phytologist

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$ on growth and reproductive performance confirmed the sensitivity of the population to the pollutant. In the absence of the development of typical visible symptoms of foliar damage, the total d. wt of plants maintained in O $ over a 56-d period was 35 % lower than that of control plants. However, the impact of the pollutant was found to decrease as plants aged. Plant relative growth rate (R E ) was only affected in seedlings, suggesting that effects of O $ on seedling growth were largely responsible for the decrease in accumulated biomass ; the growth rate of older plants was not affected by O $ . The observed shift in O $ resistance with plant age was mediated by both ' acclimation ' and ontogenetic changes. ' Acclimation ' was not associated with changes in O $ uptake, and there was some evidence to support the existence of compensatory growth responses. In addition to effects on vegetative growth, plants exhibited an O $ -induced decline in reproductive performance ; O $ reducing the number of flower spikes and seed capsules produced per plant. Ozone episodes administered at different stages of development indicated that reproductive development was particularly sensitive to O $ during the early stages of flowering.The findings of this study are discussed in relation to evolutionary adaptation to O $ in natural plant communities.The importance of plant age, prior exposure to the pollutant and the timing of O $ episodes in relation to plant developmental stage are highlighted.

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“…Ascorbate reacts readily with O $ , superoxide, singlet oxygen and H # O # and thus plays an important role in protecting cellular components from oxidative stress (Smirnoff & Pallanca, 1996). Furthermore, recent work conducted by Conklin et al 1996) on a semi-dominant monogenic mutant of Arabidopsis thaliana (soz1 ; since renamed vtc1 by Conklin et al, 1997) provides strong evidence that at least one of the genes involved in O $ resistance is connected with the capacity to synthesize this key antioxidant.…”

Section: mentioning

confidence: 99%

Impacts of ozone on Plantago major: apoplastic and symplastic antioxidant status

1999

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The aim of this work was to examine the correspondence between apoplastic\symplastic antioxidant status and previously reported plant age-related shifts in the ozone (O $ ) resistance of Plantago major L. Seed-grown plants were fumigated in duplicate controlled environment chambers with charcoal\Purafil2-filtered air (CFA) or CFA plus 70 nmol mol −" O $ for 7 h d −" over a 42 d period. Measurements of stomatal conductance and antioxidants were made after 14, 28 and 42 d fumigation, on leaves at an equivalent stage of development (youngest fully expanded leaf, measured c. 9 d after emergence). Ozone exposure resulted in a similar decline in stomatal conductance across plant ages, indicating that increases in O $ resistance with plant age were mediated through changes in the tolerance of leaf tissue rather than enhanced pollutant exclusion. Leaf apoplastic washing fluid was found to contain ' unspecific ' peroxidase, ascorbate peroxidase, superoxide dismutase and ascorbate, but not glutathione and the enzymes required to facilitate the regeneration of ascorbate from its oxidized forms. A weak induction in the activity of certain symplastic antioxidants was found after 14 d O $ fumigation, despite a lack of visible symptoms of injury, but shifts in symplastic antioxidant enzyme activity were not consistent with previously observed increases in resistance to O $ with plant age. By contrast, changes in ' unspecific ' peroxidase activity and in the small pool of ascorbate in the leaf apoplast were found to accompany age-related shifts in O $ resistance. It is concluded that constituents of the leaf apoplast may constitute a potentially important front line defence against O $ .

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Ascorbate metabolism in relation to oxidative stress

Cited by 176 publications

References 20 publications

Comparative study of catalase-peroxidases (KatGs)

Comparative study of catalase-peroxidases (KatGs)

Influence of plant age on ozone resistance in Plantago major

Impacts of ozone on Plantago major: apoplastic and symplastic antioxidant status

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