Ascorbic acid oxidation of thiol groups from dithiotreitol is mediated by its conversion to dehydroascorbic acid

Barbosa, Nilda B.V.; Colpo, Elisangela; Klimaczewski, Cláudia V.; Lissner, Leandro A.; Rocha, Joao B.T.

doi:10.17877/de290r-5140

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Although ascorbic acid is primarily known for its antioxidant properties, it can also act as a pro-oxidant agent. This paradoxical phenomenon has been previously described with thiol groups [ 16 ].…”

Section: Discussionmentioning

confidence: 59%

Stability Study of Parenteral N-Acetylcysteine, and Chemical Inhibition of Its Dimerization

et al. 2023

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Parenteral N-acetylcysteine has a wide variety of clinical applications, but its use can be limited by a poor chemical stability. We managed to control parenteral N-acetylcysteine stability, and to study the influence of additives on the decrease of N-acetylcysteine degradation. First, an HPLC-UV dosing method of N-acetylcysteine and its main degradation product, a dimer, was validated and the stability without additive was studied. Then, the influence of several additives (ascorbic acid, sodium edetate, tocopherol and zinc) and of temperature on N-acetylcysteine dimerization was evaluated. Finally, the influence of zinc gluconate at different concentrations (administrable to patients) was investigated. Zinc gluconate at 62.5 µg·mL−1 allows the stabilization of 25 mg·mL−1 N-acetylcysteine solution for at least 8 days when stored at 5 ± 3 °C.

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Section: Discussionmentioning

confidence: 59%

Stability Study of Parenteral N-Acetylcysteine, and Chemical Inhibition of Its Dimerization

et al. 2023

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“…On the other hand, the concentrations of PON-1 in RBCs lysates and plasma and thiol in all sample types decreased when incubated with higher concentrations of ascorbic acid. It has been demonstrated that acid ascorbic can oxidize thiols by its auto-oxidation to dehydroascorbic acid, and, by consequence, the decreased PON1 activity due to the reduction in its free SH groups number could explain the findings of this study [ 51 , 52 ].…”

Section: Discussionmentioning

confidence: 68%

Measurement of Redox Biomarkers in the Whole Blood and Red Blood Cell Lysates of Dogs

et al. 2022

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The evaluation of the biomarkers of oxidative status is usually performed in serum, however, other samples, such as red blood cells (RBCs) lysates or whole blood (WB), can be used. The objective of this study was to evaluate if a comprehensive panel of redox biomarkers can be measured in the WB and RBCs of dogs, and their possible changes “in vitro” after the addition of different concentrations of ascorbic acid. The panel was integrated by biomarkers of the antioxidant status, such as cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type 1 (PON-1), and of the oxidant status, such as total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). All the assays were precise and accurate in WB and RBCs lysates. In addition, they showed changes after ascorbic acid addition that are in line with previously published results, being WB more sensitive to detect these changes in our experimental conditions. In conclusion, the panel of assays used in this study can be measured in the WB and RBCs of the dog. In particular, the higher sensitivity to detect changes in our experimental conditions and its easier sample preparation makes WB a promising sample for the evaluation of redox status in dogs, with also potential applications to other animal species and humans.

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Ascorbic acid oxidation of thiol groups from dithiotreitol is mediated by its conversion to dehydroascorbic acid

Cited by 2 publications

References 32 publications

Stability Study of Parenteral N-Acetylcysteine, and Chemical Inhibition of Its Dimerization

Stability Study of Parenteral N-Acetylcysteine, and Chemical Inhibition of Its Dimerization

Measurement of Redox Biomarkers in the Whole Blood and Red Blood Cell Lysates of Dogs

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