Aspectos hematológicos, bioquímicos, morfológicos e citoquímicos de células sangüíneas em Viperídeos neotropicais dos gêneros Bothrops e Crotalus mantidos em cativeiro

Flow cytometric characterization of peripheral blood leukocyte populations of 3 neotropical snake species:Boa constrictor, Bothrops jararaca, andCrotalus durissus

“…This should be taken into account when evaluating snake heterophils in flow cytometry. On the other hand, lymphocytes and azurophils were represented in cytograms in expected locations according to size and internal complexity characteristics described by light and electron microscopy …”

“…Despite of wide morphologic and cytochemical leukocyte variations in snakes, analysis based on light microscopic morphology on conventionally stained blood smears is generally sufficient to determine different cell types. However, advanced studies are required to understand cellular functions and thus better characterize each leukocyte population . The present study purpose was to optimize density gradient leukocyte isolation protocols from snake blood, and to establish flow cytometry parameters defining size and complexity to gate and characterize different leukocyte series in Boa constrictor , Bothrops jararaca , and Crotalus durissus .…”