Assay and recommendations for the detection of vapour‐phase‐mediated antimicrobial activities

Feyaerts, Adam F.; Mathé, Lotte; Luyten, Walter; Tournu, Hélène; Dyck, Katrien Van; Broekx, Lize; Dijck, Patrick Van

doi:10.1002/ffj.3400

Cited by 14 publications

(17 citation statements)

References 24 publications

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“…Feyaerts et al [67] introduced an assay method under the original name of vapour-phase-mediated patch assay for detecting vapour phase antimicrobial activity of the VA, which uses U-shaped, 96wells microtitre plates, where a patch is defined as the set of wells in an area (square) surrounding one or more test wells. A schematic diagram of the plate design is shown in ▶ Fig.…”

Section: Microplate Patch Volatilization Assaymentioning

confidence: 99%

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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This review summarizes data on the in vitro antimicrobial effectiveness of volatile agents of plant origin and in vitro methods for evaluating their activity in the vapour phase. As a result of literature analysis, the antimicrobial efficacy of vapours from 122 different plant species and 19 pure compounds examined in 61 studies using different in vitro tests against a broad spectrum of microorganisms was identified and summarized. In addition, 11 different techniques found in the literature are described in detail. An original classification of methods based on the solid and liquid matrix volatilization principle is proposed because carrier medium/matrix selection is crucial for the volatilization of any agents tested. This review should be useful for medicinal, pharmaceutical, food, and agricultural experts working in areas related to the management of infectious diseases (especially respiratory and skin infections), food preservation (active packaging), and protection of agriculture products (controlled atmosphere). It may also stimulate the interest of pharmaceutical, cosmetic, food, and agriculture industries in the research and development of new antimicrobial agents of natural origin. Since several original apparatuses previously developed for antimicrobial susceptibility testing in the vapour phase are described in this review, labware manufacturers may also be interested in this topic. The review also provides specific guidelines and recommendations for researchers studying the antimicrobial activity of volatile agents. The article will therefore appeal to communities of industrial stakeholders, pharmacists, physicians, food experts, agriculturists, and researchers in related areas such as pharmacology, medicinal chemistry, microbiology, natural product chemistry, food preservation and plant protection.

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Section: Microplate Patch Volatilization Assaymentioning

confidence: 99%

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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“…While the broth microdilution assay is considered the gold standard for the detection of antimicrobial activity in solution 35 , 36 , it fails to detect VMAA of volatiles. Moreover, unless it has been taken into account in the design of an experiment, VMAA may lead to false positive results 22 , 23 . Therefore, we developed the VMS assay which quantifies the activity of a volatile on microorganisms in liquid culture, whereas other vapour-phase assays quantify the antimicrobial activity of the vapour-phase itself 37 – 41 .…”

Section: Discussionmentioning

confidence: 99%

“…To quantify the antimicrobial activity of a molecule against a specific microorganism, the minimal inhibitory concentration (MIC) is typically determined, preferably using standardised protocols such as the broth microdilution assay 18 – 21 . However, standardised protocols are not necessarily suitable for antimicrobials with a high vapour-pressure 22 , 23 . These molecules may exhibit an antimicrobial activity at a distance that is mediated by their vapour-phase, which permits easy administration by inhalation, treatment of e .…”

Section: Introductionmentioning

confidence: 99%

“…The procedure is based on the Clinical and Laboratory Standards Institute (CLSI) protocol for the broth microdilution assay using standard 96-well plates 24 , 25 . As such, the VMS assay resembles the previously introduced vapour-phase-mediated patch assay which can be used for the detection of VMAA 23 . Both assays belong to a new class of broth microdilution based assays where a volatile is evaluated for its biological activity in a liquid culture, following its initial evaporation and migration via the vapour-phase.…”

Section: Introductionmentioning

confidence: 99%

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Essential oils and their components are a class of antifungals with potent vapour-phase-mediated anti-Candida activity

Feyaerts

Mathé

Luyten

et al. 2018

Sci Rep

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Multi-resistant microorganisms continue to challenge medicine and fuel the search for new antimicrobials. Here we show that essential oils and their components are a promising class of antifungals that can have specific anti-Candida activity via their vapour-phase. We quantify the vapour-phase-mediated antimicrobial activity (VMAA) of 175 essential oils and 37 essential oil components, representing more than a 1,000 unique molecules, against C. albicans and C. glabrata in a novel vapour-phase-mediated susceptibility assay. Approximately half of the tested essential oils and their components show growth-inhibitory VMAA. Moreover, an average greater activity was observed against the intrinsically more resistant C. glabrata, with essential oil component citronellal having a highly significant differential VMAA. In contrast, representatives of each class of antifungals currently used in clinical practice showed no VMAA. The vapour-phase-mediated susceptibility assay presented here thus allows for the simple detection of VMAA and can advance the search for novel (applications of existing) antimicrobials. This study represents the first comprehensive characterisation of essential oils and their components as a unique class of antifungals with antimicrobial properties that differentiate them from existing antifungal classes.

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“…The cinnamon tree, mainly used to produce essential oil from its bark, leaf, and fruit, is widely distributed in the Guangxi and Guangdong provinces of China . The cinnamon oils obtained from all parts of the tree are complicated mixtures, mainly including cinnamaldehyde (CA), 2‐methoxycinnamaldehyde (2‐MCA), cinnamyl acetate (CAc), and benzaldehyde (BA), etc . Moreover, the composition of cinnamon oil differs from the different parts of the cinnamon tree.…”

Section: Introductionmentioning

confidence: 99%

Facile separation of cinnamyl acetate and cinnamaldehyde based on host–guest complexation with β‐cyclodextrin

Chai

Zheng

et al. 2018

Flavour & Fragrance J

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A selective inclusion of cinnamyl acetate (CAc) is observed during the complexation of CAc and cinnamaldehyde (CA) with β‐cyclodextrin (β‐CD) in aqueous solution. To demonstrate the potential application of β‐CD for the separation of CAc and CA, the preferential affinity of β‐CD for CAc over CA was studied from conformational and thermodynamic points of view. Determination of the complexation geometries, performed by 1H and 2D (XRD), confirmed the inclusion capacity of the β‐CD cavity for CAc and CA. From the stability constants measured by ultraviolet‐visible (UV–Vis) spectroscopy and thermodynamic parameters measured by isothermal titration microcalorimetry, it was found that compared to CA, hydrophobic and hydrogen bonding interactions jointly promote CAc to form a more stable β‐CD binding complex with 1:1 stoichiometry. Furthermore, the higher binding affinity of β‐CD for CAc (over CA) was investigated theoretically in terms of binding energy and inclusion geometries. The separation of the equimolar CAc–CA mixture was achieved by inclusion complexation in β‐CD aqueous solution. The effects of the experimental conditions, such as the polarity of the solvent and guest/host molar ratio, on separation performance were investigated. Under the optimized conditions, the selective inclusion procedure exhibited a CAc/CA separation factor of 3.5, which remained stable after five cycles, indicating that β‐CD is a highly reusable host material.

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Assay and recommendations for the detection of vapour‐phase‐mediated antimicrobial activities

Cited by 14 publications

References 24 publications

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Essential oils and their components are a class of antifungals with potent vapour-phase-mediated anti-Candida activity

Facile separation of cinnamyl acetate and cinnamaldehyde based on host–guest complexation with β‐cyclodextrin

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