The workload remained one of the major problems in the health care system. All Health professionals dealing with this issue of increased workload such as doctors, nurses, Paramedic’s etc. Objective: To assess the effect of workload on patient safety and quality of care in the medical and surgical units of Jinnah Hospital, Lahore. Methods: A descriptive cross sectional research study design was conducted among 178 patients at Jinnah Hospital, Lahore. The convenient sampling technique was used. The population that is targeted was patients of Jinnah Hospital, Lahore. Results: The general finding of the study demonstrated that workload affect the patient safety and quality of care. The descriptive statistics applied, frequency and mean checked, formed for quality, cronbach alpha, bartlett's, KMO and kolmogorov-smirnov test values have been checked to insure the validity and reliability and data normality in our context. The values showed positive and significant results and tools were consider as reliable and valid for performing statistical analysis. Conclusions: The study concluded that nurse to patient ratio is not appropriate. And this inappropriate nurse to patient ratio cause lack of patient safety and decrease quality care. In addition to patient condition and staffing numbers, the structure of the nursing work system also has an impact on nursing workload. The outcomes demonstrated a significant relation between nursing workload and patient safety.