ASSESSING ABSORBABILITY OF BIOACTIVE COMPONENTS IN ALOE USINGIN VITRODIGESTION MODEL WITH HUMAN INTESTINAL CELL

Abstract

This study used a simulated in vitro digestion model coupled with caco‐2 cell to assess the digestive stability and absorption of aloin, aloe‐emodin and aloenin A. Aloenin A and aloe‐emodin were stable and entirely recovered during simulated digestion, but 50% of aloin was lost. Approximately 53.2, 7.3 and 28.7% of aloe‐emodin, aloenin A and aloin, respectively, was transported into both apical and basolateral compartments after 1 h incubation in caco‐2 cell. The involvement of several transporter proteins for aloin and aloenin A was examined. An inhibitor of SGLT1 on apical surface (phloridzin) or that of GLUT2 on basolateral membrane (cytochalasin B) reduced the absorption of aloin by 40 or 60%, respectively, indicating that aloin is likely to be a partial substrate of SGLT1. In the presence of an efflux transporter inhibitor (verapamil), the transport of aloenin A through an intentinal apical membrane increased up to 2.1 times compared with the control (without verapamil). PRACTICAL APPLICATIONS Our results on both digestive stability and intestinal absorption characteristics of bioactive components in aloe could be of helpful information for promoting its bioavailability. The in vitro technique described in this study provides a rapid and cost‐effective alternative for predicting bioavailability of biomarkers in aloe functional food.

Determination of S‐methyl‐L‐methionine (SMM) from Brassicaceae Family Vegetables and Characterization of the Intestinal Transport of SMM by Caco‐2 Cells

“…It was reported that primary pharmacological activity of phlorizin was to inhibit the sodium‐glucose symporters found in the small intestinal membrane, blocking intestinal glucose absorption (Ehrenkranz and others ). Phloridzin as a competitive inhibitor of SGLT1 was coincubated with SMM for evaluating involved transport mechanism (Vaidyanathan and Walle ; Shim and Kwon ). In order to examine whether SMM is a substrate for SGLT1 transporter or not, cellular uptake of SMM by Caco‐2 cells was determined by incubation with SGLT1 competitive inhibitor such as a phloridzin.…”

“…Bioavailability is normally described as the potential amount of components in food matrix, which can be available into body (Ekmekcioglu ; Laparra and others ; Shim and Kwon ). Caco‐2 cell lines as a model of human intestines have been well recognized to predict the bioavailability of bioactive components in utilized food matrix or plants (Ekmekcioglu ; Laparra and others ; Shim and Kwon ; Yang and others ). For increasing the bioavailability of SMM, it is important to understand its intestinal transport mechanism.…”

CHANGES IN PROFILING OF PHENOLIC COMPOUNDS, ANTIOXIDATIVE EFFECT AND TOTAL PHENOLIC CONTENT INSMILAX CHINAUNDERIN VITROPHYSIOLOGICAL CONDITION

“…Previous studies have confirmed that an in vitro digestion model system simulating human digestive fluids could support reliable prediction of bioavailability or transport mechanism of polyphenols in various plants (Shim and Kwon 2010a,b).…”

Traditional and medicinal use of Barbaloin: potential for the management of various diseases

“…If there is an increase in the rates of elimination of blood alcohol throughout the body were originate with the treatment of barbaloin. Pretreatments with the help of can results in decrease the level of blood alcohol (Area under curve) and there is an increase in the rate of departure of ethanol [36]. To evaluate the digestive constancy or in the absorption of barbaloin with other aloe constituents, the in vitro model fitted with cancer coli-2cell was used in the current identification.…”