Assessing adsorbent–biomass interactions during expanded bed adsorption onto ion exchangers utilizing surface energetics

Vennapusa, RamiReddy; Hunegnaw, Sara M.; Cabrera, Rodrigo; Fernández‐Lahore, Marcelo

doi:10.1016/j.chroma.2007.11.078

Cited by 36 publications

(62 citation statements)

References 34 publications

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“…Zeta-potential measurements were performed with a ZetaSizer Nano-ZS (Malvern instruments, Worcestershire, United Kingdom), as previously described [12]. Zeta-potential values were gathered employing biomass pretreated as described before (under Section 2.3.1) and utilizing the same buffers utilized for contact angle determination.…”

Section: Zeta-potential Determinationmentioning

confidence: 99%

“…When integrated into mathematical expressions accounting the geometric constraints existing between two interacting bodies, G values can be utilized to calculate the corresponding energy-distance profile (U vs. H). Details of this procedure were published [12,17]. G LW are also related to the Hamaker constant, as follows:…”

Section: Energy-distance Profile Calculationsmentioning

confidence: 99%

“…Zeta-potential values are measured by electrophoretic mobility experiments [12]. Calculations were performed employing a commercial software package (GraphPad Prism, GraphPad Software Inc., San Diego, CA, USA).…”

Section: Energy-distance Profile Calculationsmentioning

confidence: 99%

“…A more comprehensive approach to understand biomass deposition onto chromatographic supports has been proposed by utilizing principles of colloid theory to explain biomass-adsorbent attachment at the local (particle) level [12]. This approach is based on extended DLVO calculations performed via experimentally determination of contact angles and z-potential values for the interacting surfaces or particles.…”

Section: Introductionmentioning

confidence: 99%

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Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds

Vennapusa

Tarı

Cabrera

et al. 2009

Biochemical Engineering Journal

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a b s t r a c tCell-to-support interaction and cell-to-cell aggregation phenomena have been studied in a model system composed of intact yeast cells and Phenyl-Streamline adsorbents. Biomass components and beaded adsorbents were characterized by contact angle determinations with three diagnostic liquids and zeta potential measurements. Subsequently, free energy of interaction vs. distance profiles between interacting surfaces was calculated in the aqueous media provided by operating mobile phases. The effect of pH and ammonium sulphate concentration within the normal operating ranges was evaluated. Calculation indicated that moderate interaction between cell particles and adsorbent beads can develop in the presence of salt. Cell-to-cell aggregation was suspected to occur at high salt concentration and neutral pH. Predictions based on the application of the XDLVO approach were confirmed by independent experimental methods like biomass deposition experiments and laser diffraction spectroscopy. Understanding biomass attachment onto hydrophobic supports can help in alleviating process limitations normally encountered during expanded bed adsorption of bioproducts.

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Section: Zeta-potential Determinationmentioning

confidence: 99%

Section: Energy-distance Profile Calculationsmentioning

confidence: 99%

Section: Energy-distance Profile Calculationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds

Vennapusa

Tarı

Cabrera

et al. 2009

Biochemical Engineering Journal

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“…Yet it depends on the further downstream operation that follows the cell disruption. High particle size with low viscosity would be useful for centrifugal separation and dead-end filtration [1,14] , but low particle size accompanied with low viscosity would be beneficial for anionic expanded bed adsorption [18,[21][22][23][24] . On the other hand, the characteristics of homogenates is not an affecting factor in cross flow filtration [19] .…”

Section: Maximum Protein and Ifn-α2b Releasementioning

confidence: 99%

Classification of Pressure Range Based on the Characterization of Escherichia coli Cell Disruption in High Pressure Homogenizer

Nagasun¹

2009

American J. of Biochemistry and Biotechnology

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Problem statement: High pressure Homogenizer was used for cell disruption in many studies. But no work was carried out to study the characteristics of cell disruption in a wide range of pressure. Approach: The characteristics of Escherichia coli cell disruption was studied in Avestin small scale homogenizer by varying the operating pressure (50-1500 bar), cell concentration in the feed (1.39-12.51 g dry cell weight L −1 ) and number of passes (1-5 passes). Results: It was found that cell concentration between 1.39 g dry cell weight L −1 and 12.51 g dry cell weight L −1 has no effect on cell disruption while the pressure applied and number of passes gave different effects on cell disruption characteristics. In between 100 and 250 bar, the protein release was mainly due to point break. In this case, the variation in cell size was not significant with increasing number of passes and maximum protein release was not achieved even after many numbers of pass. However, selectivity of specific protein (interferon-α2b) was high as it is located predominantly in periplasmic region. In between 1000 and 1500 bar, the maximum protein release, maximum interferon-α2b release and drastic reduction of cell size was observed after the first pass. In subsequent passes, micronization of cell debris was observed but without much variation in protein release. There was no reduction in antigenicity of interferon-α2b even at 1500 bar. At 500 bar, the protein release and reduction of cell size were significantly increased with increasing number of passes. Conclusion: The pressure range for E. coli cell disruption was classified as low pressure range (100-250 bar), transition pressure (500 bar) and high pressure range (1000-1500 bar). The working pressure for the homogenizer could be selected by considering the operating cost and further downstream processing.

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Expanded Bed Chromatography, Surface Energetics of Biomass Deposition

Fernández‐Lahore

Aguilar

Vennapusa

et al. 2010

Encyclopedia of Industrial Biotechnology

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The introduction of Expanded Bed Adsorption (EBA) system has made biochemical engineers face the challenge of operating a “chromatography” in the presence of biomass and other particulate components which are normally present in a crude, unclarified, feedstock. This situation can lead to detrimental consequences in terms of increased adsorbent fouling, decreased adsorbent binding capacity, and poor bed hydrodynamics. Biomass and other suspended biological material can be very sticky, depending on the type of adsorbent employed and the solution chemistry involved and thus, can create severe limitations during processing. A better local understanding of biomass deposition, including the two separate cell‐to‐bead (“interaction”) and cell‐to‐cell (“aggregation”) phenomena has been developed to allow for safer EBA operations. To this end, we propose a surface energetics‐based approach. As per the XDLVO theory, free interfacial energy versus distance profiles were calculated for a large combination of model biomass and adsorbent bead types. The energy minima values (absolute value/ U ) obtained for each of the analyzed cases were correlated with the corresponding deposition coefficient values (α). A positive correlation was obtained. It was concluded that total interaction energy U ≤ − 25 to − 50 kT and biomass deposition parameter α ≤ 0.15 would be a safe region for EBA operation; for example, biomass interference can be neglected. Deviation in the correlation was found in such cases where moderate or high cell‐to‐cell aggregation occurs, for example, in hydrophobic interaction systems. In the later cases, laser diffraction studies were necessary to fully characterize the system. These novel tools will help in alleviating the current EBA technical constraints and will guide the design of more efficient adsorbent types.

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Assessing adsorbent–biomass interactions during expanded bed adsorption onto ion exchangers utilizing surface energetics

Cited by 36 publications

References 34 publications

Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds

Surface energetics to assess biomass attachment onto hydrophobic interaction adsorbents in expanded beds

Classification of Pressure Range Based on the Characterization of Escherichia coli Cell Disruption in High Pressure Homogenizer

Expanded Bed Chromatography, Surface Energetics of Biomass Deposition

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