This study was conducted to investigate the effect of insecticides with different mode of actions and differenttoxicity levels against coccinellids and spiders in rice ecosystem at Regional Agricultural Research Station,Warangal during rabi, 2020-21 with the test cultivar Telangana Sona (RNR-15048). The selected granularand foliar insecticides viz., flubendiamide 0.7G, flubendiamide 20 WDG, cartap hydrochloride 4G, cartaphydrochloride 50 SP, chlorantraniliprole 0.4G, chlorantraniliprole 18.5 SC, fipronil 0.3G, fipronil 5 SC andcarbofuran 3G (check insecticide) were evaluated for their safety against the populations of Coccinellids and spiders in rice ecosystem. The insecticides were applied twice, i.e, granular formulations were appliedeither in nursery or 25 DATor both while spray formulations were used at 60 DAT (at panicle initiation tobooting stage). Out of 14 treatments, maximum population of beneficials (coccinellids and spiders) wereregistered in untreated control (T14) followed by carbofuran (T13) with 38.83% reduction, fipronil (T10, T11,T12) and chlorantraniliprole (T7, T8 and T9) ranging from 40.80 to 63.22% reduction whereas Flubendiamide (T1, T2 and T3) and cartap hydrochloride (T4, T5 and T6) were found to have greater impact on beneficialfauna (58.78 to 77.78% reduction over control). From the study it was evident that carbofuran, fipronil andchlorantraniliprole were found relatively safer to beneficial populations in rice ecosystem.