Assessing CaMPARI as new approach methodology for evaluating neurotoxicity

Biechele-Speziale, Dana; Camarillo, Manuel A.; Martin, Nathan R.; Biechele-Speziale, John; Lein, Pamela J.; Plavicki, Jessica

doi:10.1016/j.neuro.2023.05.013

Cited by 8 publications

(7 citation statements)

References 66 publications

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“…To offer a solution to the synthesis-related challenges and costs of molecular data storage, we set out to encode data using chemicals diverted from waste streams. Several waste sources were obtained from two academic laboratories: one developing organic synthesis methodology and one studying the developmental effects of exposure to environmental contaminants . In contrast with most other molecular data storage systems, prior knowledge of the chemical makeup of the source materials was not required for their use.…”

Section: Resultsmentioning

confidence: 99%

“…Several waste sources were obtained from two academic laboratories: one developing organic synthesis methodology 22 and one studying the developmental effects of exposure to environmental contaminants. 23 In contrast with most other molecular data storage systems, prior knowledge of the chemical makeup of the source materials was not required for their use. The samples were diluted in dimethyl sulfoxide (DMSO), and a matrix-assisted laser desorption ionization (MALDI) matrix was added to the mixtures.…”

Section: Resultsmentioning

confidence: 99%

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Repurposing Waste Chemicals for Sustainable and Durable Molecular Data Storage

Gumus,

Biechele-Speziale,

Manz

et al. 2024

ACS Omega

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Molecular data storage offers the intriguing possibility of higher theoretical density and longer lifetimes than today's electronic memory devices. Some demonstrations have used deoxyribonucleic acid (DNA), but bottlenecks in nucleic acid synthesis continue to make DNA data storage orders of magnitude more expensive than electronic storage media. Additionally, despite its potential for long-term storage, DNA faces durability challenges from environmental degradation. In this work, we demonstrate nongenomic molecular data storage using molecular libraries redirected from chemical waste streams. This approach requires no synthetic effort and can be implemented by using molecules that have a minimal associated cost. While the technique is agnostic about the exact molecular content of its inputs, we confirmed that some sources contained poly fluoroalkyl substances (PFAS), which persist for long periods in the natural environment and could offer extremely durable information storage as well as environmental benefits. These demonstrations provide a perspective on some of the valuable possibilities for nongenomic molecular information systems.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Repurposing Waste Chemicals for Sustainable and Durable Molecular Data Storage

Gumus,

Biechele-Speziale,

Manz

et al. 2024

ACS Omega

Self Cite

View full text Add to dashboard Cite

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“…Importantly, the degree of red-to-green fluorescence is contingent upon the levels of calcium allowing for the precise capture of neuronal activity in response to specific stimuli, both in vivo and in vitro . While CaMPARI probes have not been as extensively used as GCaMPs, they have proven to be valuable tool for the screens aimed at assessing the neurotoxicity of environmental contaminants or pharmaceuticals ( Kanyo et al, 2021 ; Biechele-Speziale et al, 2023 ). Recently, live imaging of zebrafish larvae expressing CaMPARI was instrumental in elucidating the mechanism of Kv7 voltage-gated potassium channel inhibition ( Kanyo et al, 2023 ).…”

Section: Methods To Study Brain Activity and Behaviormentioning

confidence: 99%

Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights

Doszyn,

Dulski,

Zmorzynska

2024

Front. Mol. Neurosci.

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The zebrafish (Danio rerio) is increasingly used in neuroscience research. Zebrafish are relatively easy to maintain, and their high fecundity makes them suitable for high-throughput experiments. Their small, transparent embryos and larvae allow for easy microscopic imaging of the developing brain. Zebrafish also share a high degree of genetic similarity with humans, and are amenable to genetic manipulation techniques, such as gene knockdown, knockout, or knock-in, which allows researchers to study the role of specific genes relevant to human brain development, function, and disease. Zebrafish can also serve as a model for behavioral studies, including locomotion, learning, and social interactions. In this review, we present state-of-the-art methods to study the brain function in zebrafish, including genetic tools for labeling single neurons and neuronal circuits, live imaging of neural activity, synaptic dynamics and protein interactions in the zebrafish brain, optogenetic manipulation, and the use of virtual reality technology for behavioral testing. We highlight the potential of zebrafish for neuroscience research, especially regarding brain development, neuronal circuits, and genetic-based disorders and discuss its certain limitations as a model.

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“…In this regard, several useful tools have emerged in recent years in the zebrafish field that can be applied in vivo during behavioral testing. These techniques include optogenetic and chemogenetic neuronal activation and silencing [226,259], calcium imaging [260,261], and neuronal laser ablation [7,262], and they also involve genetic manipulations with tools such as CRISPR [263,264] and morpholino oligonucleotides [265,266]. These techniques applied prior to behavioral testing can probe the contribution of specific neuronal populations, including those expressing neuropeptides and functionally related genes, to drug-induced disturbances in particular behaviors.…”

Section: Direct Relation Of Disturbances In Neurons To Changes In Zeb...mentioning

confidence: 99%

Utility of the Zebrafish Model for Studying Neuronal and Behavioral Disturbances Induced by Embryonic Exposure to Alcohol, Nicotine, and Cannabis

Collier,

Abdulai,

Leibowitz

2023

Cells

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It is estimated that 5% of pregnant women consume drugs of abuse during pregnancy. Clinical research suggests that intake of drugs during pregnancy, such as alcohol, nicotine and cannabis, disturbs the development of neuronal systems in the offspring, in association with behavioral disturbances early in life and an increased risk of developing drug use disorders. After briefly summarizing evidence in rodents, this review focuses on the zebrafish model and its inherent advantages for studying the effects of embryonic exposure to drugs of abuse on behavioral and neuronal development, with an emphasis on neuropeptides known to promote drug-related behaviors. In addition to stimulating the expression and density of peptide neurons, as in rodents, zebrafish studies demonstrate that embryonic drug exposure has marked effects on the migration, morphology, projections, anatomical location, and peptide co-expression of these neurons. We also describe studies using advanced methodologies that can be applied in vivo in zebrafish: first, to demonstrate a causal relationship between the drug-induced neuronal and behavioral disturbances and second, to discover underlying molecular mechanisms that mediate these effects. The zebrafish model has great potential for providing important information regarding the development of novel and efficacious therapies for ameliorating the effects of early drug exposure.

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Assessing CaMPARI as new approach methodology for evaluating neurotoxicity

Cited by 8 publications

References 66 publications

Repurposing Waste Chemicals for Sustainable and Durable Molecular Data Storage

Repurposing Waste Chemicals for Sustainable and Durable Molecular Data Storage

Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights

Utility of the Zebrafish Model for Studying Neuronal and Behavioral Disturbances Induced by Embryonic Exposure to Alcohol, Nicotine, and Cannabis

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