Assessing collagen alterations in enzymatic degradation models of osteoarthritis via second harmonic generation microscopy

Jambor, Alexander N.; Shelton, Emily; Kijowski, Richard; Henak, Corinne R.; Campagnola, Paul J.

doi:10.1016/j.joca.2021.08.004

Cited by 4 publications

(6 citation statements)

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“…In normal cartilage, three layers can be distinguished, which differ in the packing of collagen fibers: the upper one (the tangential superficial zone), the middle one, and the deep layer, neighboring the subchondral bone. All three layers are characterized by close values of the scattering and anisotropy coefficients, which however can change inhomogeneously during chemical and mechanical degradation during OA [42]. Nevertheless, to simplify the system, the approximation of cartilage with a homogeneous medium was used, making it possible to obtain a qualitative assessment of the influence of the scattering parameters on the shape of the DRS spectra.…”

Section: Resultsmentioning

confidence: 99%

Grading cartilage damage with diffuse reflectance spectroscopy: Optical markers and mechanical properties

Rovnyagina

Budylin

Dyakonov

et al. 2022

Journal of Biophotonics

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Section: Resultsmentioning

confidence: 99%

Grading cartilage damage with diffuse reflectance spectroscopy: Optical markers and mechanical properties

Rovnyagina

Budylin

Dyakonov

et al. 2022

Journal of Biophotonics

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“…Few studies in the literature have investigated μ s and g values of articular cartilage bulk tissue and zones. 13,23,24 Beek et al 13 reported g of bulk rabbit cartilage tissue at 632.8 nm using a double-integrating sphere and the inverse adding doubling method. 25 Shyu et al 23 reported the effective g of bulk porcine articular cartilage estimated by optical coherence tomography.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the scattering properties of articular cartilage zones and their impact on the overall optical properties and response of the bulk tissue are not well understood. Few studies in the literature have investigated μs and g values of articular cartilage bulk tissue and zones 13 , 23 , 24 . Beek et al 13 .…”

Section: Introductionmentioning

confidence: 99%

“…reported the effective g of bulk porcine articular cartilage estimated by optical coherence tomography. Jambor et al 24 . utilized goniometric setup to estimate the μs and g of articular cartilage superficial, middle, and deep zones at 445 and 890 nm and showed how enzymatic degradation such as depletion of proteoglycan and disruption of the collagen mesh will affects these parameters.…”

Section: Introductionmentioning

confidence: 99%

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Broadband scattering properties of articular cartilage zones and their relationship with the heterogenous structure of articular cartilage extracellular matrix

Kafian-Attari,

Nippolainen,

Bergmann

et al. 2023

J. Biomed. Opt.

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.SignificanceArticular cartilage exhibits a zonal architecture, comprising three distinct zones: superficial, middle, and deep. Collagen fibers, being the main solid constituent of articular cartilage, exhibit unique angular and size distribution in articular cartilage zones. There is a gap in knowledge on how the unique properties of collagen fibers across articular cartilage zones affect the scattering properties of the tissue.AimThis study hypothesizes that the structural properties of articular cartilage zones affect its scattering parameters. We provide scattering coefficient and scattering anisotropy factor of articular cartilage zones in the spectral band of 400 to 1400 nm. We enumerate the differences and similarities of the scattering properties of articular cartilage zones and provide reasoning for these observations.ApproachWe utilized collimated transmittance and integrating sphere measurements to estimate the scattering coefficients of bovine articular cartilage zones and bulk tissue. We used the relationship between the scattering coefficients to estimate the scattering anisotropy factor. Polarized light microscopy was applied to estimate the depth-wise angular distribution of collagen fibers in bovine articular cartilage.ResultsWe report that the Rayleigh scatterers contribution to the scattering coefficients, the intensity of the light scattered by the Rayleigh and Mie scatterers, and the angular distribution of collagen fibers across tissue depth are the key parameters that affect the scattering properties of articular cartilage zones and bulk tissue. Our results indicate that in the short visible region, the superficial and middle zones of articular cartilage affect the scattering properties of the tissue, whereas in the far visible and near-infrared regions, the articular cartilage deep zone determines articular cartilage scattering properties.ConclusionThis study provides scattering properties of articular cartilage zones. Such findings support future research to utilize optical simulation to estimate the penetration depth, depth-origin, and pathlength of light in articular cartilage for optical diagnosis of the tissue.

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“…In combination with SHG images, gray-level textures have been used to assess collagen alterations in tissue models of osteoarthritis, the features gathered by the analysis used to train a linear discriminant model [87]. Other gray textures have also been used, in combination with SHG imagery, and machine learning models, such as a study that looked at the link between image texture and polarization in histopathology [88].…”

Section: Gray-level Textures Have Been Used In the Automated Classifi...mentioning

confidence: 99%

Characterization and Classification of Fibrillar Collagen Networks using Gray-Level Texture Analysis

Poole¹

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Biological tissues are a combination of cellular and acellular components. The extracellular matrix (ECM), or the acellular component of tissues, is often overlooked.Tracking changes in the ECM grants insight into how aging, diseases and treatments might affect tissue structures and cellular responses. Using gray-level texture analysis, we analyzed the architecture of fibrillar collagen, a major component of the ECM.Several gray-level textural features were extracted from second harmonic generation (SHG) images and used to characterize collagen network arrangements. This work provides a basis for a classification model aimed to track changes in the cellular microenvironment. This information is vital if we wish to continue exploration of how diseases develop in different tissues, and develop novel and effective treatments for different types of chronic diseases.Foremost, and most certainly, I would like to extend my deepest and sincere gratitude to my supervisor Dr. Leila Mostaço-Guidolin. Her guidance, effort, and determination throughout the course of my studies, and in the writing of this thesis, have been invaluable. Especially during this period of history we find ourselves: the COVID-19 Pandemic, where studies felt like an uphill battle. She helped me ride out the storm, and all the while I gained valuable experience and knowledge that I'm sure will be helpful in the bright future. More so than that, she has been a source of inspiration, in her work ethic, dedication to her profession, and seemingly tireless ability to find time for her students in the midst of her own busy schedule.While I may not have met them, or know them, as much as I would have liked to due to the restrictions of the pandemic, I would like to thank my lab group, the BioImaging and Tissue Eng Lab. From the rare, and very much enjoyable, in-person meeting, to the mid-week Zoom meetings, it was always a pleasure to connect. I would especially like to thank Natasha Kunchur for her advice while I was writing this thesis. I would like to extend my thanks to Carleton University as a whole, an institution I have grown to know rather well in the seven years I have spent studying within iii its walls (two from the comfort of home), for its financial support during this degree.To my friends, for their constant support during my studies, which once appeared like they would never finish. At the times where overwhelming stress and anxiety became too much to bear, they would always be there to provide an ear to hear my complaints, a shoulder to cry on, or provide a much needed distraction.Finally, to my family, without whose support this thesis would never have been completed. Words cannot express how much gratitude I have for your support, guidance and understanding over the last few years. Especially my parents, Christopher Poole and Judith Poole, to whom I dedicate this thesis.

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Assessing collagen alterations in enzymatic degradation models of osteoarthritis via second harmonic generation microscopy

Cited by 4 publications

References 48 publications

Grading cartilage damage with diffuse reflectance spectroscopy: Optical markers and mechanical properties

Grading cartilage damage with diffuse reflectance spectroscopy: Optical markers and mechanical properties

Broadband scattering properties of articular cartilage zones and their relationship with the heterogenous structure of articular cartilage extracellular matrix

Characterization and Classification of Fibrillar Collagen Networks using Gray-Level Texture Analysis

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