The aim of the entry was to write a substantial contribution that analyses and compares the biological pollution of indoor air, the possibilities of its assessment and the control methods. In addition, the aim of our entry was to review journals covering both commercial and residential buildings. By analysing the above topics from the existing articles, one can have the impression that air pollution is one of the most important problems that need to be solved in the modern world. Adequate air quality is important for maintaining human health, affects the health of ecosystems, including animals, and determines crop production. With the development of civilisation, the quality of air in the atmosphere and indoors is constantly deteriorating. Indoor air pollution can be divided into physical (e.g., noise, inadequate lighting, ionising radiation), chemical (e.g., tobacco smoke, household products) and microbiological (bacteria, viruses, fungi and products of their metabolism) factors. Each of these factors can have a negative impact on a person’s health or cause premature death. The entry deals with indoor air pollution, focussing on biological pollutants. It compares different methods available and describes the method of sampling to analyse indoor air pollution and ways to reduce it.