Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis

Huong, Can Thu; Anh, Truong Thi Tu; Tran, Hoang-Dung; Duong, Vu Xuan; Trung, Nguyễn Thành; Khanh, Tran Dang; Xuan, Tran Dang

doi:10.3390/agriculture10060191

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…The phenotypic characterization of the mutant population in relation to the parents is a critical part of the mutation breeding program, and a thorough evaluation of the various interrelated traits, especially quantitative traits, is essential for successful selection. It was also proposed that statistical grouping methods be used to identify and classify mutants that came from the same parents (Huong et al, 2020). Multivariate analytical techniques such as cluster analysis and principal component analysis analyze several measurements on each population in order to determine genetic diversity, regardless of morphological data.…”

Section: Discussionmentioning

confidence: 99%

Development of sorghum mutant genotypes and analysis of their agro-morphological diversity

Takele

Feyisa

Disasa

2021

Preprint

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Sorghum is one of the most important cereal grains in Africa's Sub-Saharan region, used for human consumption and animal feed, but its production is hampered by biotic and abiotic constraints, necessitating genetic improvement of sorghum. The goal of this experiment was to use the ethyl methane sulfonate induced mutation technique to develop superior genotypes for sorghum production and quality for food and industry in order to achieve food security. The concentration of EMS was initially optimized by calculating LD50. Both Argity and Dekeba genotypes were treated with six different concentrations of EMS (0.10, 0.25, 0.30, 0.50, 0.75 and 1.00 %) and three different soaking periods (7, 8 and 9 hrs). The 0.25% EMS concentration at 8 hrs was chosen because it had a 50% seed survival rate and the highest frequency of mutations to generate M1 seeds. From M2 and M3, about 4000 and 2000 mutant populations were screened, respectively. For trait characterization, 190 mutated lines were selected from a mutant population of 2000M2. The 190 mutant populations and two parents were evaluated for genetic diversity, character association, and genetic divergence. For the observed quantitative phenotypic traits, analyses of variance showed substantial inter-population difference. When compared to their parent cultivars, the study mean of six treatment populations in each cultivar showed significantly superior quantitative phenotypic traits. Plant height, flowering date, maturity date, stem diameter, grain weight, and thousand seed weight varied with 81.48, 77, 42.03, 56.11, 11.44, and 96.68 % heritability in the mutant lines, according to agro-morphological characterization. The elite mutants were identified using principal component analysis based on their agro-morphological traits, while Pearson's correlation findings showed a positive correlation between yield component traits. Finally, our research allowed the identification of new promising mutant genotypes that could be tested in multi-local trials to assess their agronomic efficiency.

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Section: Discussionmentioning

confidence: 99%

Development of sorghum mutant genotypes and analysis of their agro-morphological diversity

Takele

Feyisa

Disasa

2021

Preprint

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“…The morphological and growth responses of the 14-day old seedlings were evaluated using a procedure based on root lengths, shoot lengths, seedling fresh, and seedling dry weights [ 20 ]. The salinity tolerance status (i.e., highly tolerant, tolerant, moderately tolerant, susceptible, or highly susceptible) of the plant materials was observed and scored by following the standard evaluation score (SES) system [ 41 , 78 ] as illustrated in Figure 7 .…”

Section: Methodsmentioning

confidence: 99%

Impact of OsBadh2 Mutations on Salt Stress Response in Rice

Prodhan

Islam

Alam

et al. 2022

Plants

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Mutations in the Betaine aldehyde dehydrogenase 2 (OsBadh2) gene resulted in aroma, which is a highly preferred grain quality attribute in rice. However, research on naturally occurring aromatic rice has revealed ambiguity and controversy regarding aroma emission, stress tolerance, and response to salinity. In this study, mutant lines of two non-aromatic varieties, Huaidao#5 (WT_HD) and Jiahua#1 (WT_JH), were generated by targeted mutagenesis of OsBadh2 using CRISPR/Cas9 technology. The mutant lines of both varieties became aromatic; however, WT_HD mutants exhibited an improved tolerance, while those of WT_JH showed a reduced tolerance to salt stress. To gain insight into the molecular mechanism leading to the opposite effects, comparative analyses of the physiological activities and expressions of aroma- and salinity-related genes were investigated. The WT_HD mutants had a lower mean increment rate of malondialdehyde, superoxide dismutase, glutamate, and proline content, with a higher mean increment rate of γ-aminobutyric acid, hydrogen peroxide, and catalase than the WT_JH mutants. Fluctuations were also detected in the salinity-related gene expression. Thus, the response mechanism of OsBadh2 mutants is complicated where the genetic makeup of the rice variety and interactions of several genes are involved, which requires more in-depth research to explore the possibility of producing highly tolerant aromatic rice genotypes.

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“…Huong et al . [ 54 ] reported the highest polymorphism with 0.58 PIC value with SSR markers RM237, RM10748, and RM224.…”

Section: Qtls Discovery Using Molecular Markersmentioning

confidence: 99%

Unlocking Allelic Diversity for Sustainable Development of Salinity Stress Tolerance in Rice

Raghuvanshi

Srivastava

Verulkar

et al. 2021

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: Rice is a major cereal crop which is negatively impacted by soil-salinity, both in terms of plant growth as well as productivity. Salinity tolerant rice varieties have been developed using conventional breeding approaches, however there has been limited success which is primarily due to the complexity of the trait, low yield, variable salt stress response and good genetic resources. Furthermore, the narrow genetic base is a hindrance for further improvement of the rice varieties. Therefore, there is a greater need to screen available donor germplasm in rice for salinity tolerance related genes and traits. In this regard, genomics based techniques are useful for exploring new gene resources and QTLs. In rice, the vast allelic diversity existing in the wild and cultivated germplasm needs to be explored for improving salt tolerance. In the present review, we provide an overview of the allelic diversity in the quantitative trait loci (QTLs) like Saltol, qGR6.2, qSE3 and RNC4 as well as genes like OsHKT1;1, SKC1 (OsHKT1;5/HKT8) and OsSTL1 (salt tolerance level 1 gene) related to salt tolerance in rice. We have also discussed approaches for developing salt-tolerant cultivars by utilizing the effective QTLs or genes/alleles in rice.

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Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis

Cited by 9 publications

References 31 publications

Development of sorghum mutant genotypes and analysis of their agro-morphological diversity

Development of sorghum mutant genotypes and analysis of their agro-morphological diversity

Impact of OsBadh2 Mutations on Salt Stress Response in Rice

Unlocking Allelic Diversity for Sustainable Development of Salinity Stress Tolerance in Rice

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