Assessing shifts in microbial community structure across a range of grasslands of differing management intensity using CLPP, PLFA and community DNA techniques

Grayston, Susan J.; Campbell, Colin D.; Bardgett, Richard D.; Mawdsley, J. L.; Clegg, C. D.; Ritz, Karl; Griffiths, Bryan S.; Rodwell, J. S.; Edwards, Suzanne; Davies, W. J.; Elston, D. J.; Millard, P.

doi:10.1016/s0929-1393(03)00098-2

Cited by 348 publications

(205 citation statements)

References 47 publications

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“…This variable is recognized as having an important impact on the microbial communities in grasslands (Grayston et al, 2004). For vegetation, plant species composition coupled with grazing is an important factor governing microbial community structure in upland grasslands (Grayston et al, 2004). This is consistent with our results in the sunny situation.…”

Section: Potential Metabolic Reactivity At Community Level After Distsupporting

confidence: 91%

“…In our study, only pH explained part of the variation in the shady situation where soil conditions were slightly less homogeneous than in the sunny situation ( Figure 2 and Table 2). This variable is recognized as having an important impact on the microbial communities in grasslands (Grayston et al, 2004). For vegetation, plant species composition coupled with grazing is an important factor governing microbial community structure in upland grasslands (Grayston et al, 2004).…”

Section: Potential Metabolic Reactivity At Community Level After Distmentioning

confidence: 99%

“…Much research effort has been directed at understanding how agriculture and land use practice influence the structure and diversity of animal and microbial below-ground communities (Chabrerie et al, 2003;Clegg et al, 2003;Grayston et al, 2004;Larkin, 2003). The role of soil organisms in regulating ecosystem processes has received growing attention over the past several years and there is an increasing number of studies on questions such as how decomposers respond to disturbance regimes especially those related to agriculture (Guitian and Bardgett, 2000;Wardle et al, 1999;Yeates et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Cattle have, for example, preferred resting and dunging areas, which may be different from their preferred grazing areas (Bokdam and Gleichman, 2000). In addition, the field studies compared existing situations with little control over the fields and independent variables (pseudoexperiment) (e.g., Bardgett et al, 2001;Donnison et al, 2000;Grayston et al, 2004) and microcosms were usually used in experiments (e.g., Bardgett et al, 1999;Jordan et al, 2003). Only a few studies used controlled field experiments (Clegg et al, 2003;Harrison and Bardgett, 2004;Wardle et al, 1999;Williams et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Soil Microbial Community Changes in Wooded Mountain Pastures due to Simulated Effects of Cattle Grazing

et al. 2005

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The effect of cattle activity on pastures can be subdivided into three categories of disturbances: herbage removal, dunging and trampling. The objective of this study was to assess separately or in combination the effect of these factors on the potential activities of soil microbial communities and to compare these effects with those of soil properties and plant composition or biomass. Controlled treatments simulating the three factors were applied in a fenced area including a light gradient (sunny and shady situation): (i) repeated mowing; (ii) trampling; (iii) fertilizing with a liquid mixture of dung and urine. In the third year of the experiment, community level physiological profiles (CLPP) (Biolog Ecoplatesä) were measured for each plots. Furthermore soil chemical properties (pH, total organic carbon, total nitrogen and total phosphorus), plant species composition and plant biomass were also assessed. Despite differences in plant communities and soil properties, the metabolic potential of the microbial community in the sunny and in the shady situations were similar. Effects of treatments on microbial communities were more pronounced in the sunny than in the shady situation. In both cases, repeated mowing was the first factor retained for explaining functional variations. In contrast, fertilizing was not a significant factor. The vegetation explained a high proportion of variation of the microbial community descriptors in the sunny situation, while no significant variation appeared under shady condition. The three components of cattle activities influenced differently the soil microbial communities and this depended on the light conditions within the wooded pasture. Cattle activities may also change spatially at a fine scale and short-term and induce changes in the microbial community structure. Thus, the shifting mosaic that has been described for the vegetation of pastures may also apply for below-ground microbial communities.

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Section: Potential Metabolic Reactivity At Community Level After Distsupporting

confidence: 91%

Section: Potential Metabolic Reactivity At Community Level After Distmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Soil Microbial Community Changes in Wooded Mountain Pastures due to Simulated Effects of Cattle Grazing

et al. 2005

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“…Variações na diversidade microbiana podem ser relacionadas a fatores como a diversidade vegetal de cada área e as características químicas (pH e teores de nutrientes) e físicas (porosidade, estabilidade de agregados e estrutura) de cada solo (Grayston et al, 2004). É importante ressaltar que a utilização de um método dependente de cultura, como o Biolog, apresenta limitações, pois não considera as bactérias que não podem ser cultivadas (Ros et al, 2008).…”

Section: Resultsunclassified

Estrutura metabólica e genética de comunidades bacterianas em solo de cerrado sob diferentes manejos

Souza

Schlemmer

Alencar

et al. 2012

Pesq. agropec. bras.

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O objetivo deste trabalho foi avaliar a estrutura metabólica e genética de comunidades bacterianas em Latossolo de cerrado sob vegetação nativa ou cultivado em sistema de rotação soja/milho sob preparo convencional e plantio direto. Foram utilizadas microplacas EcoPlate para determinar o perfil e a diversidade metabólica das comunidades bacterianas, e eletroforese em gel com gradiente desnaturante (DGGE) para avaliar a estrutura genética. O teste estatístico de Mantel foi utilizado para avaliar a relação entre a estrutura metabólica e a genética. A comunidade bacteriana sob vegetação nativa apresentou perfil metabólico diferente do encontrado em solos cultivados. No solo cultivado com soja sob preparo convencional, o padrão de utilização das fontes de carbono diferenciou-se dos demais tratamentos. Com base nos resultados de DGGE, a comunidade bacteriana sob vegetação nativa apresentou 35% de similaridade com as de áreas cultivadas. Foram formados grupos distintos de comunidades bacterianas do solo entre as áreas sob preparo convencional e plantio direto. Houve correlação significativa de 62% entre as matrizes geradas pelas microplacas EcoPlate e pela DGGE. Variações no perfil metabólico estão relacionadas às variações na estrutura genética das comunidades bacterianas do solo.

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The Use of Stable Isotope Labeling and Compound‐Specific Analysis of Microbial Phospholipid Fatty Acids to Quantify the Influences of Rhizodeposition on Microbial Community Structure and Function

Paterson

2013

Molecular Microbial Ecology of the Rhizosphere

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Assessing shifts in microbial community structure across a range of grasslands of differing management intensity using CLPP, PLFA and community DNA techniques

Cited by 348 publications

References 47 publications

Soil Microbial Community Changes in Wooded Mountain Pastures due to Simulated Effects of Cattle Grazing

Soil Microbial Community Changes in Wooded Mountain Pastures due to Simulated Effects of Cattle Grazing

Estrutura metabólica e genética de comunidades bacterianas em solo de cerrado sob diferentes manejos

The Use of Stable Isotope Labeling and Compound‐Specific Analysis of Microbial Phospholipid Fatty Acids to Quantify the Influences of Rhizodeposition on Microbial Community Structure and Function

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