Assessing species admixtures in raw drug trade of Phyllanthus, a hepato-protective plant using molecular tools

Srirama, R; Umapathy, Senthilkumar; Sreejayan, N.; Ravikanth, G.; Gurumurthy, B. R.; Shivanna, M. B.; Sanjappa, M.; Ganeshaiah, K. N.; Shaanker, R. Uma

doi:10.1016/j.jep.2010.04.042

Cited by 95 publications

(69 citation statements)

References 26 publications

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“…Some of the applied areas of barcoding are recognizing insect-host relationship (Jurado-Rivera et al 2009), analyzing the diet of herbivores (Valentini et al 2009;Staudacher et al 2011;Stech et al 2011), scrutinizing the components of herbal medicines (Srirama et al 2010), food products (Jaakola et al 2010) and in ecological forensics to identify the plant from a small tissue of root, or seedling or cryptic life stages (e.g., of fern gametophytes) and endangered species.…”

Section: Introductionmentioning

confidence: 99%

Discriminatory power of rbcL barcode locus for authentication of some of United Arab Emirates (UAE) native plants

et al. 2017

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DNA barcoding of United Arab Emirates (UAE) native plants is of high practical and scientific value as the plants adapt to very harsh environmental conditions that challenge their identification. Fifty-one plant species belonged to 22 families, 2 monocots, and 20 eudicots; a maximum number of species being legumes and grasses were collected. To authenticate the morphological identification of the wild plant taxa, rbcL and matK regions were used in the study. The primer universality and discriminatory power of rbcL is 100%, while it is 35% for matK locus for these plant species. The sequences were submitted to GenBank; accession numbers were obtained for all the rbcL sequences and for 6 of matK sequences. We suggest rbcL as a promising barcode locus for the tested group of 51 plants. In the present study, an inexpensive, simple method of identification of rare desert plant taxa through rbcL barcode is being reported.

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Section: Introductionmentioning

confidence: 99%

Discriminatory power of rbcL barcode locus for authentication of some of United Arab Emirates (UAE) native plants

et al. 2017

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“…The variability of the gene sequences is analyzed to determine a unique ''barcode'' for each species (Hebert et al, 2003). The technique is currently used for detecting plant-based adulteration in many commodities including medicinal plants, commercial tea packets, olive oil, and medicinal spices (Gismondi et al, 2013;Kumar et al, 2011;Mattia et al, 2010;Stoeckle et al, 2011;Srirama et al, 2010;Vijayan & Tsou, 2010;Yuan et al, 2011). A recent study (Parvathy et al, 2014) has shown the sensitivity of this technique to detect adulteration as low as 0.5% in market samples of black pepper.…”

Section: Introductionmentioning

confidence: 99%

Detection of plant-based adulterants in turmeric powder using DNA barcoding

Parvathy

Swetha

Sheeja

et al. 2015

Pharmaceutical Biology

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Context: In its powdered form, turmeric [Curcuma longa L. (Zingiberaceae)], a spice of medical importance, is often adulterated lowering its quality. Objective: The study sought to detect plant-based adulterants in traded turmeric powder using DNA barcoding. Materials and methods: Accessions of Curcuma longa L., Curcuma zedoaria Rosc. (Zingiberaceae), and cassava starch served as reference samples. Three barcoding loci, namely ITS, rbcL, and matK, were used for PCR amplification of the reference samples and commercial samples representing 10 different companies. PCR success rate, sequencing efficiency, occurrence of SNPs, and BLAST analysis were used to assess the potential of the barcoding loci in authenticating the traded samples of turmeric. Results: The PCR and sequencing success of the loci rbcL and ITS were found to be 100%, whereas matK showed no amplification. ITS proved to be the ideal locus because it showed greater variability than rbcL in discriminating the Curcuma species. The presence of C. zedoaria could be detected in one of the samples whereas cassava starch, wheat, barley, and rye in other two samples although the label claimed nothing other than turmeric powder in the samples. Discussion and conclusion: Unlabeled materials in turmeric powder are considered as adulterants or fillers, added to increase the bulk weight and starch content of the commodity for economic gains. These adulterants pose potential health hazards to consumers who are allergic to these plants, lowering the product's medicinal value and belying the claim that the product is gluten free. The study proved DNA barcoding as an efficient tool for testing the integrity and the authenticity of commercial products of turmeric.

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“…As a result, CBOL proposed to review the research to assess the performance of these 2 markers after 18 months, and suggested to continue the study on other markers during this review period. Recently, the trnH-psbA intergenic region was reported to differentiate many medicinal plants, including 17 species of Dendrobium (Yao et al, 2009), 16 species of Phyllanthus (Srirama et al, 2010), and 8 species of Panax (Zuo et al, 2011). In addition, research in China has documented success in barcoding medicinal plants using ITS2 (Chen et al, 2010;China Plant BOL Group et al, 2011;Sui et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A tiered barcode authentication tool to differentiate medicinal Cassia species in India

Purushothaman¹,

Sg²,

Ragupathy³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. DNA barcoding is a desirable tool for medicinal product authentication. DNA barcoding is a method for species identification using short DNA sequences that are conserved within species, but variable between species. Unlike animals, there is no single universal DNA barcode locus for plants. Coding markers, matK and rbcL, and noncoding markers, trnH-psbA (chloroplast) and ITS2 (nuclear), have been reported to be suitable for the DNA barcoding of plants with varying degree of success. Sixty-four accessions from 20 species of the medicinal plant Cassia were collected, and analyzed for these 4 DNA barcoding markers. PCR amplification was 100% successful for all 4 markers, while intra-species divergence was 0 for all 4 Cassia species in which multiple accessions were studied. Assuming 1.0% divergence as the minimum requirement for discriminating 2 species, the 4 markers could only differentiate 15 to 65% of the species studied when used separately. Adding indels to the divergence increased the percentage of species discrimination by trnH-psbA to 90%. In 2-locus barcoding, while matK+rbcL (which is recommended by Consortium for the Barcoding of Life) discriminated 90% of the species, the other combinations of matK+ITS and rbcL+trnH-psbA showed 100% species discrimination. However, matK is plagued with primer issues. The combination of rbcL+trnH-psbA provided the most accurate (100% species ID) and efficient tiered DNA barcoding tool for the authentication of Cassia medicinal products.

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Assessing species admixtures in raw drug trade of Phyllanthus, a hepato-protective plant using molecular tools

Cited by 95 publications

References 26 publications

Discriminatory power of rbcL barcode locus for authentication of some of United Arab Emirates (UAE) native plants

Discriminatory power of rbcL barcode locus for authentication of some of United Arab Emirates (UAE) native plants

Detection of plant-based adulterants in turmeric powder using DNA barcoding

A tiered barcode authentication tool to differentiate medicinal Cassia species in India

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