Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics

Salgueiro, Vanessa; Reis, Lígia; Ferreira, Eugénia; Botelho, Maria João; Manageiro, Vera; Caniça, Manuela

doi:10.3390/antibiotics10091135

Cited by 12 publications

(9 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the spatiotemporal study of bacterial resistance against florfenicol in the area of intense salmon farming, the microbiota of mussel M. chilensis revealed a striking influence depending on the distance to the next fish farm concerning water currents ( Ramírez et al, 2022 ). Another study pinpoints AR against antibiotics used in aquaculture but against colistin–an antibiotic of last resort against multidrug-resistant Gram-negative bacteria in human medicine in Portuguese mussel farms ( Salgueiro et al, 2021 ).…”

Section: Anthropogenic Impact On Marine Microbiotamentioning

confidence: 99%

Molluscs—A ticking microbial bomb

Kijewska

Koroza²,

Grudlewska‐Buda³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Bivalve shellfish consumption (ark shells, clams, cockles, and oysters) has increased over the last decades. Following this trend, infectious disease outbreaks associated with their consumption have been reported more frequently. Molluscs are a diverse group of organisms found wild and farmed. They are common on our tables, but unfortunately, despite their great taste, they can also pose a threat as a potential vector for numerous species of pathogenic microorganisms. Clams, in particular, might be filled with pathogens because of their filter-feeding diet. This specific way of feeding favors the accumulation of excessive amounts of pathogenic microorganisms like Vibrio spp., including Vibrio cholerae and V. parahaemolyticus, Pseudomonas aeruginosa, Escherichia coli, Arcobacter spp., and fecal coliforms, and intestinal enterococci. The problems of pathogen dissemination and disease outbreaks caused by exogenous bacteria in many geographical regions quickly became an unwanted effect of globalized food supply chains, global climate change, and natural pathogen transmission dynamics. Moreover, some pathogens like Shewanella spp., with high zoonotic potential, are spreading worldwide along with food transport. These bacteria, contained in food, are also responsible for the potential transmission of antibiotic-resistance genes to species belonging to the human microbiota. Finally, they end up in wastewater, thus colonizing new areas, which enables them to introduce new antibiotic-resistance genes (ARG) into the environment and extend the existing spectrum of ARGs already present in local biomes. Foodborne pathogens require modern methods of detection. Similarly, detecting ARGs is necessary to prevent resistance dissemination in new environments, thus preventing future outbreaks, which could threaten associated consumers and workers in the food processing industry.

show abstract

Section: Anthropogenic Impact On Marine Microbiotamentioning

confidence: 99%

Molluscs—A ticking microbial bomb

Kijewska

Koroza²,

Grudlewska‐Buda³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In marine habitats, antibiotics are applied to reduce or remove potential pathogenic bacteria from organisms of interest, such as fish ( Cabello, 2006 ; Salgado-Caxito et al, 2022 ; Yukgehnaish et al, 2020 ), oysters ( Baralla et al, 2021 ; Salgueiro et al, 2021 ), and corals ( Aeby et al, 2019 ; Hartman, Blackall & van Oppen, 2022 ; Neely et al, 2020 ). Some groups of animals, including ecosystem engineers like corals, are particularly susceptible to rapid environmental changes and may become susceptible to opportunistic pathogens ( Gardner et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Antibiotics alter development and gene expression in the model cnidarian Nematostella vectensis

Krueger,

Phippen,

Reitzel

2024

PeerJ

View full text Add to dashboard Cite

Background Antibiotics are commonly used for controlling microbial growth in diseased organisms. However, antibiotic treatments during early developmental stages can have negative impacts on development and physiology that could offset the positive effects of reducing or eliminating pathogens. Similarly, antibiotics can shift the microbial community due to differential effectiveness on resistant and susceptible bacteria. Though antibiotic application does not typically result in mortality of marine invertebrates, little is known about the developmental and transcriptional effects. These sublethal effects could reduce the fitness of the host organism and lead to negative changes after removal of the antibiotics. Here, we quantify the impact of antibiotic treatment on development, gene expression, and the culturable bacterial community of a model cnidarian, Nematostella vectensis. Methods Ampicillin, streptomycin, rifampicin, and neomycin were compared individually at two concentrations, 50 and 200 µg mL−1, and in combination at 50 µg mL−1 each, to assess their impact on N. vectensis. First, we determined the impact antibiotics have on larval development. Next Amplicon 16S rDNA gene sequencing was used to compare the culturable bacteria that persist after antibiotic treatment to determine how these treatments may differentially select against the native microbiome. Lastly, we determined how acute (3-day) and chronic (8-day) antibiotic treatments impact gene expression of adult anemones. Results Under most exposures, the time of larval settlement extended as the concentration of antibiotics increased and had the longest delay of 3 days in the combination treatment. Culturable bacteria persisted through a majority of exposures where we identified 359 amplicon sequence variants (ASVs). The largest proportion of bacteria belonged to Gammaproteobacteria, and the most common ASVs were identified as Microbacterium and Vibrio. The acute antibiotic exposure resulted in differential expression of genes related to epigenetic mechanisms and neural processes, while constant application resulted in upregulation of chaperones and downregulation of mitochondrial genes when compared to controls. Gene Ontology analyses identified overall depletion of terms related to development and metabolism in both antibiotic treatments. Discussion Antibiotics resulted in a significant increase to settlement time of N. vectensis larvae. Culturable bacterial species after antibiotic treatments were taxonomically diverse. Additionally, the transcriptional effects of antibiotics, and after their removal result in significant differences in gene expression that may impact the physiology of the anemone, which may include removal of bacterial signaling on anemone gene expression. Our research suggests that impacts of antibiotics beyond the reduction of bacteria may be important to consider when they are applied to aquatic invertebrates including reef building corals.

show abstract

“…L. marginalis specimens, collected at random from seemingly healthy populations, frequently harbor a diverse collection of bacteria originat-ing from both aquatic and anthropogenic sources. This bacterial community encompasses a range of species from various genera, including Vibrio, Pseudomonas, Moraxella, Aeromonas, Micrococcus, Acinetobacter, and Bacillus [15]. Bivalve molluscs have the potential to be contaminated by these bacterial pathogens, posing significant safety risks when consumed raw or undercooked, as it can lead to human illness.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Extended-Spectrum β-Lactamases (ESBLs) Producing Aeromonas spp. Isolated from Lamellidens marginalis (Lamark, 1819) of Sewage-Fed Wetland: A Phenotypic and Genotypic Approach

Mohanty,

Das,

Kumari

et al. 2024

Microorganisms

View full text Add to dashboard Cite

The global rise of zoonotic bacteria resistant to multiple antimicrobial classes and the growing occurrence of infections caused by Aeromonas spp. resistant to β-lactam antibiotics pose a severe threat to animal and human health. However, the contribution of natural environments, particularly aquatic ecosystems, as ideal settings for the development and spread of antimicrobial resistance (AMR) is a key concern. Investigating the phenotypic antibiotic resistance and detection of β-lactamase producing Aeromonas spp. in Lamellidens marginalis, which inhabit all freshwater ecosystems of the Indian subcontinent, is essential for implications in monitoring food safety and drug resistance. In the present investigation, 92 isolates of Aeromonas spp. were recovered from 105 bivalves and screened for their antimicrobial resistance patterns. In vitro antibiotic resistance profiling showed a higher Multiple Antibiotic Resistance (MAR) index of 0.8 with the highest resistance against ampicillin/sulbactam (82%), while 58, 44, 39 and 38% of the isolates were resistant to cephalothin, erythromycin, cefoxitin and imipenem, respectively. PCR results revealed that these isolates carried the blaTEM gene (94%), which was followed by the blaCTX-M gene (51%) and the blaSHV gene (45%). A combination of blaSHV, blaCTX-M, and blaTEM genes was found in 17% of the isolates, indicating the presence of all three resistance genes. This is the first investigation which highlights the importance of multidrug-resistant Aeromonas spp. in L. marginalis. The identification of extended-spectrum-β-lactamases (ESBLs) genes demand the necessity of continuous surveillance and systematic monitoring, considering its potential health risks for both animals and human beings.

show abstract

Assessing the Bacterial Community Composition of Bivalve Mollusks Collected in Aquaculture Farms and Respective Susceptibility to Antibiotics

Cited by 12 publications

References 67 publications

Molluscs—A ticking microbial bomb

Molluscs—A ticking microbial bomb

Antibiotics alter development and gene expression in the model cnidarian Nematostella vectensis

Prevalence of Extended-Spectrum β-Lactamases (ESBLs) Producing Aeromonas spp. Isolated from Lamellidens marginalis (Lamark, 1819) of Sewage-Fed Wetland: A Phenotypic and Genotypic Approach

Contact Info

Product

Resources

About