Assessing the Bioactivity and Antioxidative Properties of Some Compounds Isolated From Abutilon Hirtum (Lam.)

Abstract

Objectives: The present study on the methanol extract of Abutilon hirtum (Lam.) [Malvaceae] afforded ten compounds. Findings from this assessment indicated that A. hirtum leaves possessed vast potential as medicinal product especially in liver cancer treatment.Methods: Dried-powdered leaves were boiled under reflux in 10 L of petroleum ether for 8 hrs. After filtration, the solvent was evaporated; afforded 15 g of petroleum ether extract followed by boiling with reflux for 8 hrs with chloroform, then filtration and the residue was evaporated to give 34 g chloroform extract. Ethyl acetate was added and refluxed for 8 hrs, then filtration and evaporation to give 31 g ethyl acetate extract. Finally the leaves were refluxed with 10 L of 85 % aqueous MeOH, after cooling, the solution was filtered and evaporated and the residue was 210 g methanol extract then the residue was dissolved in de-ionized water (250 ml), then the salt was removed by adding excess methanol solution (2.5 L), and finally filtered. The cytotoxicity of the isolated compounds was evaluated towards HepG2 liver-carcinoma cell line using MTT assay, the antimicrobial activity was tested using the Disc agar plate method and the total antioxidant capacity was determined according to phosphomolybdenum method. Results:The isolated compounds identified as methyl gallate, cuneataside E, bergapten, gallic acid, ellagic acid, epigallocatechin-3-O-gallate, kaempferol-3-O-α-L-rhamnoside, benzyl-1-O-β-D-glucopyranoside, 2(4-hydroxyphenyl)ethyl-O-β-D-glucopyranoside and β-sitosterol. All the isolated compounds are known; but they were isolated from Abutilon hirtum for the first time. Conclusion:This report may serve as a footprint concerning the biological and pharmacological activities of A. hirtum leaves.

Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays

“…Additionally, the comparison between the IC50 of LEAH and standard ascorbic acid shows that the lower the IC50 values are, the lower the potential source of anticytotoxic agents. Many complementary and alternative medicines, such as A. hirtum , were found to contain polyphenols, terpenoids, and flavonoids, according to the results of previous studies (e.g., gallic acid, geraniol caryophyllene, and β-sitosterol), and epigallocatechin gallate (EGCG), which has activity in fighting cytotoxic cells in the liver and is useful as a chemotherapy [ 22 ]. The latter compound also has antioxidant activity [ 23 , 24 ].…”

“…(i.e. : gallic acid, β-sitosterol, geraniol caryophyllene), and epigallocatechin gallate (EGCG), which have activity in fighting cancer cells in the liver and are useful as chemotherapy [ 22 ]. This compound also has antioxidant activity [ 23 , 24 ].…”

“…This compound also has antioxidant activity [ 23 , 24 ]. Moreover, ellagic acid is the most effective antibacterial agent and has been well characterized in this genus [ 22 , 23 ].…”

Anticancer activity and other biomedical properties of β‐sitosterol: Bridging phytochemistry and current pharmacological evidence for future translational approaches

“…β‐sitosterol extracted from Jacaranda oxyphylla exhibited strong antimicrobial effects against Bacillus cereus , S. aureus , E. coli , Salmonella typhimurium , and Candida albicans (Pereira et al, 2016). Some of these microbes have also been used by Hamed et al (2017) to evaluate the bioactivity of β‐sitosterol purified from methanol extract of Abutilon hirtum . Nyila et al (2012) prepared ethyl acetate extract of Acacia karroo and isolated β‐sitosterol as pure compound.…”