Assessing the histological changes in fish gills as environmental bioindicators in Paraty and Sepetiba bays in Rio de Janeiro, Brazil

Carvalho, Thatiana Luiza Assis de Brito; Nascimento, Aparecida Alves do; Gonçalves, Caio Fábio dos Santos; Santos, Marcos Antônio José dos; Sales, Armando

doi:10.3856/vol48-issue4-fulltext-2351

Cited by 20 publications

(10 citation statements)

References 53 publications

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“…The results of other investigations show that MPs provoke the fusion of the two sides of the lamellae (Rahim & Yaqin, 2022). According to some authors, this could be due to the hyperplasia phenomenon (Carvalho et al, 2020). The spaces between the ciliated cells of the ciliary disks could be formed as a consequence of the breaking of the cellular junctions as has been demonstrated after pollutant exposure (Sunila, 1987).…”

Section: Discussionmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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Section: Discussionmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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“…Almost similar histopathological changes were documented by some other researchers, where they investigated the effects of different heavy metals such as arsenic, copper, cadmium, mercury and chromium on the histopathology of the gills of tilapia, Catla catla, Cirrhinus mrigala, Ctenopharyngodon idella, etc. [16,24,26,27,[66][67][68]. The gill is an important and major organ in fish for respiration, excretion and osmoregulation [6,69].…”

Section: Discussionmentioning

confidence: 99%

Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, Labeo rohita of Bangladesh

Rabbane

Kabir²,

Habibullah-Al-Mamun³

et al. 2022

Fishes

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This study was designed to elucidate the effects of arsenic (As) on the morpho-behavior, growth development and molecular mechanisms of a commercially important fish, rohu carp, Labeo rohita, in Bangladesh. Fish fry with an average weight of 387.5 ± 169.25 mg and an average length of 3.35 ± 0.37 cm were collected from a local hatchery in Mymensingh, Bangladesh and acclimatized for a week in the Department of Fisheries, University of Dhaka before starting the exposure with arsenic. Fishes were exposed for a period of 14 days with three treatments of NaAsO2, namely treatment 1(T1)—2.5 mg/L; treatment 2 (T2)—15 mg/L; and treatment 3 (T3)—30 mg/L, along with a control (C)—0.0 mg/L, with three replicates. These concentrations were determined based on the LC50 value for 96 h measured for this experiment. This study revealed remarkable morphological abnormalities and deformities in arsenic-exposed rohu carp. In fish exposed to 30 mg/L, caudal fin erosion was a frequent deformity. There was no significant difference in RNA:DNA ratio among the treatments. The overall weight of fish was decreased as the concentration of arsenic was increased. The T3 fish had a statistically significant negative weight gain (−0.05 ± 0.07 g), but the other treatments (T1 and T2) and control fish had no significant weight gain. Different types of histopathological changes were observed in the gills and intestines of arsenic-treated fish. Necrosis and severe damages were found in the secondary lamellae of gills at the highest arsenic concentration (30 mg/L). Epithelial lifting, irregular shape and damages in the gill raker were also observed in the primary lamellae of the gills for the same treatment. In this study, the expression of heat shock protein (HSP 60) and metallothionein (MT) genes was assessed by qPCR, and these genes were upregulated in different treatments compared to controls. The findings of the present study suggest that arsenic pollution significantly changes the morphology, behavior, growth, development, histopathology and molecular mechanisms of this economically important fish, rohu carp, in Bangladesh.

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“…Hyperplasia causes proliferation of adjacent lamellae cells, reducing the inter-lamella space, resulting in fusion of the lamellae [27]. Carvalho et al [28] suggest secondary lamellar fusion is likely due to severe lamellar hyperplasia leading to partial or total fusion of lamellar capillaries within a hyperplastic epithelial mass. According to Santos et al [27], damage to gill tissue can be a gill defense mechanism against stressors in the polluted aquatic environment.…”

Section: Discussionmentioning

confidence: 99%

Acute Toxicity of domestic landfill leachate to carp fish (Cyprinus carpio)

Pratiwi

Rahardja

Tjahjaningsih

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Leachate has the potential to pollute rivers because it contains high organic and inorganic materials. Acute toxicity to fish may occur in the short term. This study aims to determine the effect of acute leachate toxicity on mortality and histopathological features of carp gill organs. This study used the Completely Randomized Design (CRD) used five leachate treatments (0.5%; 1%; 1.5%; 2% and 2.5%) with four replications. Each treatment used ten fish. The results showed that the higher the leachate concentration, the higher the mortality of carp. Even mortality reached 100% occurred in groups of fish exposed to 2.5% leachate for 96 hours. Histopathological changes in carp gill organs showed hyperplasia, hypertrophy, edema, and congestion due to exposure to leachate.

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Assessing the histological changes in fish gills as environmental bioindicators in Paraty and Sepetiba bays in Rio de Janeiro, Brazil

Cited by 20 publications

References 53 publications

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, Labeo rohita of Bangladesh

Acute Toxicity of domestic landfill leachate to carp fish (Cyprinus carpio)

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