Assessing the three-dimensional collagen network in soft tissues using contrast agents and high resolution micro-CT: Application to porcine iliac veins

Nierenberger, Mathieu; Rémond, Yves; Ahzi, Saı̈d; Choquet, Philippe

doi:10.1016/j.crvi.2015.04.009

Cited by 35 publications

(44 citation statements)

References 39 publications

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“…Staining the sample for more than 36 hr results in excessive attenuation in both PTA and PMA case. Another collagen investigation, which was about porcine veins and collagen networks using two different contrast agents, was reported by Nierenberger, Rémond, and Ahzi (). In the research, fiber architecture and wall structure of the veins were examined by using three different contrast agents.…”

Section: Molecule‐based Contrast Agentsmentioning

confidence: 97%

Evaluation of X‐ray tomography contrast agents: A review of production, protocols, and biological applications

Koç

Aslan

Kao

et al. 2019

Microscopy Res & Technique

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X‐ray computed tomography is a strong tool that finds many applications both in medical applications and in the investigation of biological and nonbiological samples. In the clinics, X‐ray tomography is widely used for diagnostic purposes whose three‐dimensional imaging in high resolution helps physicians to obtain detailed image of investigated regions. Researchers in biological sciences and engineering use X‐ray tomography because it is a nondestructive method to assess the structure of their samples. In both medical and biological applications, visualization of soft tissues and structures requires special treatment, in which special contrast agents are used. In this detailed report, molecule‐based and nanoparticle‐based contrast agents used in biological applications to enhance the image quality were compiled and reported. Special contrast agent applications and protocols to enhance the contrast for the biological applications and works to develop nanoparticle contrast agents to enhance the contrast for targeted drug delivery and general imaging applications were also assessed and listed.

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Section: Molecule‐based Contrast Agentsmentioning

confidence: 97%

Evaluation of X‐ray tomography contrast agents: A review of production, protocols, and biological applications

Koç

Aslan

Kao

et al. 2019

Microscopy Res & Technique

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“…Prior to µCT scanning at 17 µm isotropic voxel resolution, the entire organism of about 6 cm in length was immersed for four weeks in an ethanol solution with added phosphotungstic acid [83]. It would be of great interest to further explore the usefulness of this µCT-based approach for the 3D visualization of the pulp cavity and all soft tissues surrounding the human tooth, in particular since new protocols for the staining of specific tissues are now increasingly becoming available [84,85].…”

Section: Soft Tissue Imaging Using Micro-computed Tomographymentioning

confidence: 99%

Three-dimensional imaging of internal tooth structures: Applications in dental education

Kato

Ziegler

Utsumi

et al. 2016

Journal of Oral Biosciences

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BackgroundProviding comprehensive knowledge of the anatomy of human teeth is one of the basic functions of dental education, because a thorough understanding of their internal structure is one of the prerequisites for any successful clinical intervention. Several techniques have traditionally been employed to study and visualize the complex internal organization of human teeth. In contrast to these invasive techniques, modern imaging systems permit non-invasive analyses of the three-dimensional structure of human teeth. Owing to the relative ease of acquisition, handling, and distribution of the respective data, digital imaging techniques will also significantly influence dental education. HighlightIn this article, a broad overview of traditional and modern techniques for the visualization of internal tooth structures is provided. Emphasis is placed on micro-computed tomography systems and their application in endodontics. In addition, 3 the results derived from a comparison of histology and micro-computed tomography are presented. Apart from its utility in basic research, digital data can also be employed to create interactive three-dimensional models that are particularly suitable for teaching purposes. ConclusionNon-invasive, three-dimensional imaging techniques, in particular the various modalities of computed tomography, have ushered in a new era of endodontic studies.Owing to their digital nature, the results derived from these techniques can not only be easily analyzed and distributed but also be rapidly integrated into the teaching materials. Recent studies as well as the data shown in the present contribution suggest that digital educational materials lead to an improved understanding of the complex anatomy of human teeth.

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“…Subsequent to Metscher's published staining protocols, PTA has been used to visualize soft tissue structures in microCT images of a variety of species and tissue types, using a multitude of different protocols with varying degrees of success (Table ). The idea that staining time with PTA was not a critical factor was repeated, so long as staining time was sufficient to penetrate tissues . However, few details were published that would indicate the length of time needed to sufficiently penetrate tissues of different species and ages.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7] Histology uses thin slices of embedded material that can be stained to highlight different cells or cellular components. A 3D block of biological material must necessarily be destroyed, by embedding the material in a harder substance that enables thin sections (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) μm thick) to be produced from the block. The timeconsuming process of specimen preparation and sectioning introduces the potential for artifacts that can obscure or distort the tissues and tissue relationships, including tearing, fracturing, or folding of the tissue.…”

Section: Introductionmentioning

confidence: 99%

“…[10][11][12] Combining microCT with specific staining protocols takes advantage of the increased resolution of microCT and mimics the soft tissue visualization capabilities of MRM. A number of different chemical stains that enhance contrast in anatomical tissues have been investigated, including osmium tetroxide and phosphomolybdic acid (PMA), 1,[13][14][15] but the majority of the literature has focused on iodine and phosphotungstic acid (PTA) with varying results and recommendations depending on the model organism, specimen age and size, tissue type, and staining protocol. 2,6,[15][16][17][18][19][20][21][22][23][24][25][26] Iodine has emerged as the more popular option, with most published protocols using Lugol's iodine (I 2 KI), although some researchers have experimented with iodine in absolute ethanol (I 2 E).…”

Section: Introductionmentioning

confidence: 99%

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Phosphotungstic acid‐enhanced microCT: Optimized protocols for embryonic and early postnatal mice

Lesciotto

Perrine

Kawasaki

et al. 2019

Developmental Dynamics

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Background Given the need for descriptive and increasingly mechanistic morphological analyses, contrast‐enhanced microcomputed tomography (microCT) represents perhaps the best method for visualizing 3D biological soft tissues in situ. Although staining protocols using phosphotungstic acid (PTA) have been published with beautiful visualizations of soft tissue structures, these protocols are often aimed at highly specific research questions and are applicable to a limited set of model organisms, specimen ages, or tissue types. We provide detailed protocols for micro‐level visualization of soft tissue structures in mice at several embryonic and early postnatal ages using PTA‐enhanced microCT. Results Our protocols produce microCT scans that enable visualization and quantitative analyses of whole organisms, individual tissues, and organ systems while preserving 3D morphology and relationships with surrounding structures, with minimal soft tissue shrinkage. Of particular note, both internal and external features of the murine heart, lungs, and liver, as well as embryonic cartilage, are captured at high resolution. Conclusion These protocols have broad applicability to mouse models for a variety of diseases and conditions. Minor experimentation in the staining duration can expand this protocol to additional age groups, permitting ontogenetic studies of internal organs and soft tissue structures within their 3D in situ position.

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Assessing the three-dimensional collagen network in soft tissues using contrast agents and high resolution micro-CT: Application to porcine iliac veins

Cited by 35 publications

References 39 publications

Evaluation of X‐ray tomography contrast agents: A review of production, protocols, and biological applications

Evaluation of X‐ray tomography contrast agents: A review of production, protocols, and biological applications

Three-dimensional imaging of internal tooth structures: Applications in dental education

Phosphotungstic acid‐enhanced microCT: Optimized protocols for embryonic and early postnatal mice

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