Assessing tritium internalisation in zebrafish early life stages: Importance of rapid isotopic exchange

Arcanjo, Caroline; Maro, D.; Camilleri, Virginie; Cavalié, Isabelle; Simon, Olivier; Beaugelin­-Seiller, K.; Carasco, Loïc; Orjollet, Daniel; Adam-Guillermin, Christelle; Gagnaire, Béatrice

doi:10.1016/j.jenvrad.2019.02.009

Cited by 16 publications

(6 citation statements)

References 17 publications

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“…Several studies showed that tritium accumulated in adult organisms equilibrates with HTO concentration of the water and that the fraction of tritium bound to organic matter equilibrates with the concentration of HTO internalized (Galeriu and Melintescu ; Kim and Korolevych ; Melintescu et al ; Kim et al , ; Gagnaire et al , ). A companion paper study presented in detail the discussion about internalization results obtained after tritium contamination for 2 stages (24‐h eggs and 4‐dpf larvae) and 2 HTO concentrations (10 5 and 10 6 Bq/mL; Arcanjo et al ).…”

Section: Discussionmentioning

confidence: 99%

“…To calculate dose rates, internalization of tritium was assessed using a protocol which aimed to minimize the exchanges between organisms and ambient medium developed in a related study (Arcanjo et al ). Dose rates were calculated using tritium activity concentrations measured in the exposure medium (external dose rate) and in egg or larvae (internal dose rate) applying dose coefficients calculated with EDEN Ver 2 software (IRSN), according to the hypothesis of a homogeneous tritium distribution in tissues (Table ).…”

Section: Methodsmentioning

confidence: 99%

“…Several studies showed that tritium accumulated in adult organisms 7: Relative expression of genes (normalized to reference gene ef1) compared to control in larvae contaminated during 10 d at 10 4 , 10 5 , and 10 6 Bq/mL of tritiated water (n = 9) a 4 dpf 7 dpf 10 dpf 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL 10 4 Bq/mL 10 5 Bq/mL 10 6 Bq/mL ache equilibrates with HTO concentration of the water and that the fraction of tritium bound to organic matter equilibrates with the concentration of HTO internalized (Galeriu and Melintescu 2011;Kim and Korolevych 2013;Melintescu et al 2015;Kim et al 2015Kim et al , 2019Gagnaire et al 2017Gagnaire et al , 2020. A companion paper study presented in detail the discussion about internalization results obtained after tritium contamination for 2 stages (24-h eggs and 4-dpf larvae) and 2 HTO concentrations (10 5 and 10 6 Bq/mL; Arcanjo et al 2019).…”

Section: Dose Rates Calculated For Htomentioning

confidence: 96%

“…The histopathology of the tail muscle was also studied. Total dose rates were calculated for eggs and larvae by considering an external dose rate calculated using HTO concentration and an internal dose rate calculated using tritium activities measured in individuals, using a protocol developed in a related study (Arcanjo et al ). The results were then compared with observations obtained after exposure of zebrafish larvae to gamma irradiation in previous studies.…”

Section: Introductionmentioning

confidence: 99%

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Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Gagnaire

Arcanjo

Cavalié

et al. 2020

Enviro Toxic and Chemistry

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Tritium, a radioactive isotope of hydrogen of natural and anthropogenic origin, is ubiquitously present in the environment. Effluents of nuclear centers of production are significant anthropogenic sources. With the upcoming project of thermonuclear fusion, tritium releases in the environment may increase. It is therefore important to characterize the ecological risk linked to tritium. The effects of tritiated water (HTO) were therefore studied in zebrafish larvae exposed for 10 d to different dose rates, 1.1 × 102, 4.1 × 102, and 3.8 × 103 µGy/h for larvae corresponding, respectively, to a water contamination of 104, 105, and 106 Bq/mL of HTO. Those dose rates were higher than 10 μGy/h, which is the threshold recommended to start monitoring ecosystems where radiological contaminants are present. Mortality, embryo‐larval development, immune toxicity, genotoxicity, neurotoxicity, and alterations of tissues were investigated. The results showed that HTO exposure induced DNA damage and reactive oxygen species production and modulated the expression of genes involved in detoxification processes. Moreover, modifications of the muscular tissues (degradation of myofibrils at 4 d post fertilization and disorganization of mitochondria at later stages) were observed. The results differed with HTO dose rates and with developmental stages. These results will drive future research for the development of new HTO‐sensitive biomarkers and will allow us to progress in the characterization of the modes of action of tritium in fish. Environ Toxicol Chem 2020;39:648–658. © 2019 SETAC

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Dose Rates Calculated For Htomentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Gagnaire

Arcanjo

Cavalié

et al. 2020

Enviro Toxic and Chemistry

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“…Control organisms were handled in the same way. This protocol was optimised for zebrafish (Arcanjo et al, 2019).…”

Section: Eggs Contamination and Samplingmentioning

confidence: 99%

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

et al. 2020

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Distributed under a Creative Commons Attribution-NonCommercial-NoDerivatives| 4.0 International License

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Tritiated thymidine induces developmental delay, oxidative stress and gene overexpression in developing zebrafish (Danio rerio)

Schiano Di Lombo,

Cavalié,

Camilleri

et al. 2023

Aquatic Toxicology

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Assessing tritium internalisation in zebrafish early life stages: Importance of rapid isotopic exchange

Cited by 16 publications

References 17 publications

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

Tritiated thymidine induces developmental delay, oxidative stress and gene overexpression in developing zebrafish (Danio rerio)

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