Assessment of antimicrobial resistance patterns in Escherichia coli isolated from clinical samples in Madinah, Saudi Arabia

Saeed, Walaa M.; Ghanem, Samah; Shafey, Hatem M. El; Manzoor, Nikhat

doi:10.5897/ajmr2018.8836

Cited by 8 publications

(2 citation statements)

References 19 publications

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“…Multidrug-resistance (MDR) by bacteria has become a public health issue, which is estimated to cause maximum deaths by the year 2050 along with increasingly high health expenses. A rise in antimicrobial resistance has been reported in E. coli worldwide, resulting in complications and treatment issues [5]. According to Tule and Hassani [6], E. coli isolates from neonates without any prior exposure to antibiotics are highly resistance to antibiotics like ampicillin (100%) and co-trimoxazole (96%).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Property of Ethanolic Leaf Extract of Eucalyptus citriodora Hook on Clinical and Typed Isolates of Escherichia coli

Muhammed

Dada

Alo

2018

SAJRM

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The emergence of antibiotic resistance bacteria, necessitated the discovery and development of alternative therapy to bacterial infections. This work assessed the inhibitory property of ethanolic leaf extract of Eucalyptus citriodora against clinical isolate and E. coli ATCC 35218. Agar well diffusion test was used to determine the antibacterial activity of the extract on both isolates of E. coli. For both isolates, no zone of inhibition was observed at concentrations of the extract between 50-150 mg/ml, but at 200-500 mg/ml, there were significant (P>0.05) zones of inhibitions that ranged between 4.2 – 13.7 and 4.7 – 15.4 mm for clinical isolate and E. coli ATCC 35218 respectively. The susceptibility of both isolates to conventional antibiotics revealed ciprofloxacin (10 ug) having the highest inhibitions against both isolates (17.3 mm and 13.9 mm) respectively, followed by gentamycin (14.4 mm and 10.8 mm). The clinical isolate was resistant to amoxicillin (30 ug), while E. coli ATCC 35218 was susceptible (4.3 mm). The MIC of the extract for both isolates was 200 mg/ml while the MBCs were 300 and 350 mg/ml respectively. The inhibitory activities of the extract (15.4 mm and 13.7mm) at 500 mg/ml concentration is comparable with ciprofloxacin (17.3 mm and 13.9 mm) at a concentration of 10 µg, while that of Gentamycin at 30 µg (14.4 mm and 10.8 mm) is comparable with concentration of the extract at 450 mg/ml (13.7 mm and 11.3 mm). The zones of inhibitions produced by the extract increased with increasing concentrations. This study revealed the potency of E. citriodora ethanolic leaf extract as a future herbal candidate to treat infection caused by E. coli at high concentrations of the extract.

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Section: Introductionmentioning

confidence: 99%

“…Escherichia coli is ubiquitous and present in both animals and the environment [5]. It is Gram negative, facultatively anaerobic, rod-shaped, coliform commonly found in the intestinal tract of warm-blooded animals including humans.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Property of Ethanolic Leaf Extract of Eucalyptus citriodora Hook on Clinical and Typed Isolates of Escherichia coli

Muhammed

Dada

Alo

2018

SAJRM

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Escherichia coli in Saudi Arabia: An Overview of Antibiotic-Resistant Strains

Alharbi¹

2020

Biosci., Biotech. Res. Asia

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Escherichia coli (E. coli) bacterial strains are considered as the most important human pathogens. Health issues are increasing in complexity owing to the persistent emergence of antibiotic resistant E. coli strains, which have been isolated and detected worldwide, including Saudi Arabia. A review of the prevalent strains resistant to the standard antibiotics used in a local region is critical and may be beneficial at the national and international levels. Treatment for E. coli infection has been highly difficult by the rise of resistance to most first-line antibiotics. The present study aimed to update the scientific information regarding E. coli strains, which have the ability to resist the standard drugs used to treat bacterial infections in Saudi Arabia. The data retrieved from https://scholar.google.com and Saudi Digital Library (https://sdl.edu.sa/) indicate that E. coli strains isolated from several sources in Saudi Arabia show resistance to almost all antibiotics, except 5th generation cephalosporins (ceftraolin and ceftobiprole), which no isolate in Saudi Arabia has been recorded to resist. Based on the results of the present study, we conclude and recommend that integrated monitoring and management of the antibiotics may reduce the health risks associated with antibiotic resistant E. coli strains.

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Pathogenic spectrum of blood stream infections and resistance pattern in Gram-negative bacteria from Aljouf region of Saudi Arabia

Bandy

Almaeen

2020

PLoS ONE

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Background The pathogenic spectrum of bloodstream infections (BSIs) varies across regions. Monitoring the pathogenic profile and antimicrobial resistance is a prerequisite for effective therapy, infection control and for strategies aimed to counter antimicrobial resistance. The pathogenic spectrum of BSIs in blood cultures was analysed, focusing on the resistance patterns of Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae, in Aljouf region. Methods This descriptive cross-sectional study analysed the culture reports of all non-duplicate blood samples collected from January 1 to December 31, 2019. Antibiograms of A. baumannii, E. coli, and K. pneumoniae were analysed for antibiotic resistance. The frequency and percentages of multi-drug, extensively-drug, pan-drug and carbapenem resistance were calculated. Results Of the 222 bloodstream infections, 62.2% and 36.4% were caused by gram-negative and gram-positive bacteria, respectively. Most BSIs occurred in patients aged �60 years (59.5%). Among the 103 isolates of the studied Gram-negative bacteria (GNB), 47.6%, 38.8%, and 2.9% were multi-drug, extensively drug and pan-drug resistant respectively. 46% of K. pneumoniae isolates were carbapenemase producers. Resistance to gentamycin, 1 st-4 th generation cephalosporins, and carbapenems was observed for A. baumannii. More than 70% of E. coli isolates were resistant to 3 rd-and 4 th-generation cephalosporins. Klebsiella pneumoniae presented a resistance rate of >60% to imipenems.

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Assessment of antimicrobial resistance patterns in Escherichia coli isolated from clinical samples in Madinah, Saudi Arabia

Cited by 8 publications

References 19 publications

Antibacterial Property of Ethanolic Leaf Extract of Eucalyptus citriodora Hook on Clinical and Typed Isolates of Escherichia coli

Antibacterial Property of Ethanolic Leaf Extract of Eucalyptus citriodora Hook on Clinical and Typed Isolates of Escherichia coli

Escherichia coli in Saudi Arabia: An Overview of Antibiotic-Resistant Strains

Pathogenic spectrum of blood stream infections and resistance pattern in Gram-negative bacteria from Aljouf region of Saudi Arabia

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