Assessment of Aspergillus fumigatus pathogenicity in aerosol-challenged chickens (Gallus gallus) belonging to two lineages

Thierry, Simon; Durand, Benoı̂t; Melloul, Emmanuel; Tafani, Jean-Pierre; Wang, Dong Ying; Deville, Manjula; Cordonnier, Nathalie; Chermette, René; Guillot, Jacques; Arné, Pascal

doi:10.1016/j.cimid.2013.01.003

Cited by 13 publications

(9 citation statements)

References 30 publications

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“…4,9,10,15,16,40 Individuals belonging to the avian species listed above species be thoroughly examined for aspergillosis; however, only few experimental studies have been performed to determine true differences in susceptibility between bird species 41 or poultry lineages 42 . Recent infection trials could not confirm a predisposition based on species tested.…”

Section: Anamnesis/history/environmental Investigationmentioning

confidence: 99%

Diagnostic Procedures and Available Techniques for the Diagnosis of Aspergillosis in Birds

Fischer

Lierz

2015

Journal of Exotic Pet Medicine

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Section: Anamnesis/history/environmental Investigationmentioning

confidence: 99%

Diagnostic Procedures and Available Techniques for the Diagnosis of Aspergillosis in Birds

Fischer

Lierz

2015

Journal of Exotic Pet Medicine

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“…Eleven out of 25 infected chickens died, and most chickens infected with A. fumigatus showed overt clinical signs, such as depression, dyspnea, and ruffled feathers. In contrast, the aerosol-challenged layer chickens belonging to White Leghorn lineage showed resistance to this fungus (17). The mechanisms of induction and the type of immunity are different when pathogens by-pass the upper respiratory tract, which would determine the level of resistance or susceptibility to a pathogen.…”

Section: Discussionmentioning

confidence: 97%

“…Experimental aspergillosis has been studied in chickens for many years (14)(15)(16). Recently, Thierry et al also reported that the conventional JA657 broilers were more susceptible to A. fumigatus than the White Leghorn PA12 layers, indicating the lineage of chicken plays an important role in the pathogenicity of A. fumigatus (17). The immunopathogenesis of A. fumigatus infection in chickens has yet to be fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity and Immune Responses of Aspergillus fumigatus Infection in Chickens

Cheng

Wang

et al. 2020

Front. Vet. Sci.

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Aspergillus fumigatus is a ubiquitous pathogen in poultry farms, causing aspergillosis in chickens. To study the pathogenicity of A. fumigatus, 14-days-old chickens were infected with fungal conidia (2 × 10 7 CFU/mL) via thoracic intra-air sacs inoculation. The clinical symptoms, gross and histopathological lesions, and fungal load in the lungs were examined. Additionally, the mRNAs of Toll like receptors (TLR) and pro-inflammatory cytokines were evaluated by quantitative PCR to explore the immune responses induced by A. fumigatus. The results showed that overt depression, ruffled feathers, and dyspnea were observed in the infected chickens as early as 3 days post infection (dpi). Eleven out of 25 infected chickens died from 5 to 9 dpi, and A. fumigatus could also be reisolated from the infected lung. Histopathological examination revealed obvious airsacculitis and pneumonia, characterized by inflammatory cell infiltration (heterophils and macrophages), and granulomatous lesions in the lung. The mRNA expressions of TLR1 and TLR2 were upregulated in the lung and spleen, and most pro-inflammatory cytokines including IL-1β, Cxcl-8, TNF-α, IL-12, and IFN-γ were increased in both the lung and spleen during the tested period, suggesting that the innate immune responses were triggered by A. fumigatus infection, and these cytokines participated in the inflammatory responses against A. fumigatus. These results indicate that A. fumigatus infection by thoracic intra-air sacs inoculation can cause severe respiratory damage in chickens, activate TLR1 and TLR2 mediated immune responses, and elicit large expression of pro-inflammatory cytokines such as IL-1β, Cxcl-8, and IFN-γ. These data will help further understanding of the pathogenesis and immune responses of A. fumigatus infection in the chicken.

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“…Immune suppression is also a contributing element to the development of an Aspergillus infection . A predisposition based on species has been implicated by clinical observation and experimental studies, but was dismissed by another study . Some species might be more predisposed to infection, but in some avian species (ie , penguins), infections could be due to their poor housing environments …”

Section: Predisposing Factors and Species Susceptibilitymentioning

confidence: 99%

The current status of avian aspergillosis diagnoses: Veterinary practice to novel research avenues

Savelieff¹,

Pappalardo

Azmanis

2018

Veterinary Clinical Pathol

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Aspergillus fungal infections continue to be a significant cause of morbidity and mortality in birds that can, in part, be attributed to the lack of a diagnostic "gold standard" for Aspergillus infection, and which delays the diagnosis, treatment, and outcome of avian patients. At present, none of the available methods in veterinary care can detect aspergillosis early enough and with the accuracy, precision, and specificity required of an ideal diagnostic tool. Therefore, researching methods of Aspergillus detection is still an active area of inquiry, and novel techniques continue to emerge. This review will provide a brief overview of current clinical methods, with an emphasis on avian care, in addition to a series of techniques in development that could offer distinct advantages over existing methods.

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Assessment of Aspergillus fumigatus pathogenicity in aerosol-challenged chickens (Gallus gallus) belonging to two lineages

Cited by 13 publications

References 30 publications

Diagnostic Procedures and Available Techniques for the Diagnosis of Aspergillosis in Birds

Diagnostic Procedures and Available Techniques for the Diagnosis of Aspergillosis in Birds

Pathogenicity and Immune Responses of Aspergillus fumigatus Infection in Chickens

The current status of avian aspergillosis diagnoses: Veterinary practice to novel research avenues

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