Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers

Abstract

Costus pictus, belonging to the family Costaceae, is one of the valuable medicinal plants with its antidiabetic property. Despite ever-increasing demand from the pharmaceutical industry, this species is being less exploited at molecular level. Hence, an effort has been made in the present study to characterize the 15 accessions of C. pictus collected from different geographical regions of India through random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. A total of 25 RAPD and 20 ISSR primers were used in the present study. The RAPD analysis generated 343 loci, of which 124 were polymorphic with an average of 4.96 loci per primer. While, ISSR primers produced 177 loci, of which 77 were polymorphic with an average of 3.85 loci per primer. The similarity coefficients ranged from 0.86-0.99, 0.84-0.95 and 0.86-0.96 for RAPD, ISSR and combined RAPD-ISSR, respectively. The UPGMA dendrogram generated using these data showed low level of divergence among the accessions from South and West regions. Further, accession-specific bands were also revealed by RAPD and ISSR markers which might be contributed to specific trait. This investigation was an understanding of genetic variation within the C. pictus accessions. The present finding indicates that both the marker tools RAPD and ISSR combined or individually can be used in determining the genetic relationship between the accessions. It may be concluded that data of hereditary differences appeared among the C. pictus accessions could be utilized for their conservation and reproducing programs.

RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress

“…Resolving powers in our study were in the range of 0.63-5.11 (average 2.98) for RAPD and ISSR 1.56-8.18 (average 4.10) per primer. According to Fang and Roose (1997) and Naik et al (2017), ISSR markers were found to be more effective in diversity study than RAPD markers. The present study clearly supports the view.…”

Population Diversity of Odontotermes formosanus (Shiraki) (Termitidae, Macrotermitinae) from Different Geographic Locations in Anhui Province, China

“…In this system, PCR is performed on a short DNA sequence between two simple sequence repeat (SSR) sequences in genomic DNA with a single oligomerized nucleotide primer containing anchored 1-4 bases in the 3′ or 5′ end of the sequence, which are widely available and exhibits fast evolutionary rate (Esselman et al, 1999;Tanya et al, 2011;Hu et al, 2015). In addition, the features of long sequence and high annealing temperature ensure that ISSR-PCR would exhibit high stability and reproducibility (Nagaoka & Ogihara, 1997;Tsumura et al, 1996;Qian et al, 2001;Naik et al, 2017). Although ISSR is widely used in studies on various plants (Qian et al, 2001;Cao et al, 2006;Tanya et al, 2011), fungi (Menzies et al, 2003) and some insects Dutta et al, 2012;Barbosa et al, 2014), but it was less used on the study of termites (Long et al, 2009).…”

Genetic diversity and population structure of three commercial indigenous Aloe species in selected ASALs of Kenya

“…However, low levels of polymorphism using both ISSR and RAPD markers were reported in Bruguirra gymnorrhiza and Heritiera fomes [30]. Most studies on wild plant populations have used the percentage of polymorphism as an indicator of genetic diversity [31]. However, [32] indicated that the percentage polymorphism is not a significant measure of genetic variation and that the parameter of genetic diversity (H) is more applicable.…”