Assessment of Genotoxic Effects of Boron on Wheat (Triticum aestivum L.) and Bean (Phaseolus vulgaris L.) by Using RAPD Analysis

Kekeç, Güzin; Sakcali, Mehmet Serdal; Uzonur, Irem

doi:10.1007/s00128-010-0031-z

Cited by 38 publications

(24 citation statements)

References 17 publications

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“…This variable correlations can be explained by a range of influencing factors, including the diversity among samples, variable reaction kinetics, presence and activity of enzymes responsible for mediating oxidative stress, (Jaio and Wang 2000;Wu et al 2004;Ozgen et al 2006) and etc. In the samples treated with Ni and Cd as non-redox metals, correspondingly to previously published facts we detected high levels of (Enan 2006;Kekec et al 2010;Cenkci et al 2009). The RAPD, PCR based assay described here is fast, reliable, and easy to conduct in any laboratory for assessment of environmental hazardous metals on plants.…”

Section: Resultssupporting

confidence: 87%

“…This plant is exposed to heavy metals in its natural environment as a result of various human activities. Bean is a diploid (2n=22) and it has been widely used in physiological and molecular analysis in toxicology (Enan 2006;Kekec et al 2010). Seeds were surface-sterilized with 75 % (v/v) ethanol for 5 min, followed by 10 % (m/v) sodium hypochlorite for 10 min.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Advantages of measuring effects of genotoxic chemicals directly on DNA are mainly related to the sensitivity and short response time. DNA based techniques like Random Amplified Polymorphic DNA (RAPD) is used to evaluate the variation at the DNA level and can clearly be shown when comparing DNA fingerprints from untreated and treated individuals to genotoxic agents (Savva 1998;Atienzar and Jha 2006;Enan 2006;Kekec et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Ackova

Panovska

Ruskovska

et al. 2013

Physiol Mol Biol Plants

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The present study aimed to analyze the biological effects induced by bioaccumulation of metals in common bean (Phaseolus vulgaris L.). Effects of mineral nutrient imbalance, total antioxidants level and DNA damage induced by accumulation of heavy metals, were investigated in bean seedlings treated with two selected metal concentrations for 7 days. Metal content is analyzed by inductively coupled plasma -atomic emission spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay is used and Random Amplified Polymorphic DNA (RAPD) method was applied for investigation of DNA damages. The increasing metal concentration in the treatment medium changed synchronously metal content in samples, and decreased total antioxidant activity in all samples with exception only for samples treated with Ni and Cd. The obtained "DNA fingerprints" demonstrated that the increasing metal concentrations induced changes in RAPD profiles (disappearance and/or appearance of bands in comparison with untreated control samples). The highest number of missing bands was observed in samples treated with zinc (total 4 bands) and nickel (total 4 bands) at both concentrations. These results suggested that mineral nutrient imbalance is involved in changes of antioxidant levels and DNA damages of the seedlings, which may help to understand the mechanism of metal toxicity in plants.

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Section: Resultssupporting

confidence: 87%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Ackova

Panovska

Ruskovska

et al. 2013

Physiol Mol Biol Plants

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“…RAPD technique, as PCR-based technique, has been successfully used to detect DNA damage and mutations in plants induced by various types of toxic chemicals [8, 9, 36]. Each fragment in RAPD is derived from a region of the genome that contains two short segments in inverted orientation on opposite strands that are complementary to the primer and sufficiently close together for the amplification process [37].…”

Section: Resultsmentioning

confidence: 99%

Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage inUrtica dioica

Ackova

Panovska

Ruskovska

et al. 2013

BioMed Research International

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Heavy metals have the potential to interact and induce several stress responses in the plants; thus, effects of heavy metal stress on DNA damages and total antioxidants level in Urtica dioica leaves and stems were investigated. The samples are sampled from areas with different metal exposition. Metal content was analyzed by Inductively Coupled Plasma-Atomic Emission Spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay was used, and genomic DNA isolation from frozen plant samples was performed to obtain DNA fingerprints of investigated plant. It was found that heavy metal contents in stems generally changed synchronously with those in leaves of the plant, and extraneous metals led to imbalance of mineral nutrient elements. DNA damages were investigated by Random Amplified Polymorphic DNA (RAPD) technique, and the results demonstrated that the samples exposed to metals yielded a large number of new fragments (total 12) in comparison with the control sample. This study showed that DNA stability is highly affected by metal pollution which was identified by RAPD markers. Results suggested that heavy metal stress influences antioxidant status and also induces DNA damages in U. dioica which may help to understand the mechanisms of metals genotoxicity.

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“…For detecting genotoxicity, the RAPD-PCR (Randomly Amplified Polymorphic DNA) technique was employed and photosynthetic pigment contents were evaluated in order to make assessments for the effects of Cd toxicity. Using RAPD-PCR in assessing of genotoxic effects of heavy metals is commonly used technique, which enables to detect the DNA band features such as band intensity and gain or loss in relation to toxicant exposures (Atienzar & Jha, 2006;Swaileh et al, 2008;Kekec et al, 2010). Therefore, this study results will pave the way for understanding of alterations in genetic material in relation to heavy metal exposure such as Cd as well as in changes of metabolic processes relatedly.…”

Section: Introductionmentioning

confidence: 89%

Detection of physiological and genotoxic damages reflecting toxicity in Kalanchoe clones

Demir¹,

Tombuloğlu²,

Sakcali³

et al. 2016

Global NEST Journal

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In order to make assessments in understanding of physiological and genotoxic effects of imposing cadmium (Cd) on photosynthetic pigment contents along with the changes occurring in genetic material of Kalanchoe plants were used in relation to various Cd-treatments. Young plantlets were originated from a single host plant as clones, and developed in vitro. Developed clones were grown in standard pots with daily watering of Hoagland solution (20 ml) containing different concentrations of cadmium chloride for two months. Cd concentrations of the collected samples were measured by employing ICP-OES and RAPD-PCR technique was applied for detecting the genotoxic effects of Cd. After two month of experimental period, the comparisons between unexposed and exposed Kalanchoe clone groups revealed reductions in photosynthetic pigment contents, especially at the highest level of Cd exposure and a genomic instability when application of Cd concentration increases. RAPD-PCR analyses demonstrated the distinguishable banding pattern in number and band intensities between Cd-treated and control groups. In addition, progressive Cd accumulations in leaves, stems and roots of plant samples were observed when the application of exposure level increased.

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Assessment of Genotoxic Effects of Boron on Wheat (Triticum aestivum L.) and Bean (Phaseolus vulgaris L.) by Using RAPD Analysis

Cited by 38 publications

References 17 publications

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Influence of Heavy Metal Stress on Antioxidant Status and DNA Damage inUrtica dioica

Detection of physiological and genotoxic damages reflecting toxicity in Kalanchoe clones

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