Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Abstract

Brucellosis is a contagious systemic bacterial disease of livestock. Antibiotics commonly being used have limited efficacy against the disease. In the present study, therapy with antibiotics on Brucella infected cattle was performed and subsequently haemato-biochemical parameters, detection of anti-Brucella antibodies in both serum and milk by different diagnostics and shedding of Brucella in milk were assessed. Treatment with antibiotics; streptomycin and long acting tetracycline alone and in combination were given for four weeks. Hb, TEC, TLC, neutrophils, eosinophils, lymphocytes, monocytes count, AST, ALT and SD values were lower whereas, PCV, serum glucose, total protein, albumin and creatinine were higher in Brucella infected cattle. Treatment with combination therapy showed promising results in these animals. Haemato-biochemical parameters were become near to normal in most of animals that received combination therapy. However, not all animals were returned to normal after treatment but a significant numbers in combination therapy were reduced anti-Brucella antibody titre, both in serum and milk. The level of shedding of Brucella in milk had also gone undetectable. KeywordsBrucellosis; Cattle; Haemato-biochemical parameters; Oxytetracycline; Streptomycin Reaction (PCR) based assays have been developed for the rapid identification of Brucella. It is advantageous to detect DNA of pathogen that rendered biologically safe and hence, reduced the risk of infection to laboratory workers. In addition, PCR is more sensitive than traditional culture method [8].There is limited evidence on treatment aspect of Brucella infected cattle as well as their haemato-biochemical parameters. Brucella is inaccessible to antibiotics as they are facultative intracellular pathogens. Many antimicrobials are active against Brucella species; however, clinical efficacy does not always correlate with in vitro susceptibility [9]. Treatments for brucellosis have been tried previously but none was found successful [10,11]. Streptomycin acts synergistically with penicillin or tetracycline to inhibit the growth of B. abortus within bovine cells cultured in vitro [12]. Considering the above facts, the present work was intended to determine the efficacy of antibiotic therapy and alteration in the haemato-biochemical parameters if any, in Brucella infected cattle after treatment. Materials and MethodsA total of 27 cattle which showed antibodies both in serum and milk as well as Brucella DNA in milk were selected for the study. ELISA, RBPT and STAT were employed on serum whereas, MRT and ELISA on milk to detect the anti-Brucella antibodies. Milk samples were processed for detection of pathogen itself in form of its DNA (16S rRNA) by PCR [13]. Reference antigens and serum for RBPT, STAT and, for MRT, Abortus Bang Ring Antigen (ABR Antigen) were procured from the Indian Veterinary Research Institute, Izatnagar (India). AniGen B. Brucella Ab ELISA kit (Cat. No. EB43-01) (i-ELISA and Milk-ELISA) was procured

Levels of certain biochemical and oxidative stress parameters in cattle with Brucellosis

“…Although there are many studies on oxidative stress and biochemical parameters in brucellosis in human medicine (Ali, 2009;Serefhanoglu et al, 2009), limited number of studies are available on biochemical parameters (Kushwaha et al, 2014;Elazab, 2015) and oxidative stress (Nisbet et al, 2007;Kataria et al, 2010) in cattle with brucellosis in veterinary medicine. The purpose of the present study was to determine concentrations of some biochemical parameters and oxidative stress levels in cattle with brucellosis.…”

Assessment of Haemato-Biochemical and Therapeutic Responses of Chronic Brucellosis in Crossbred Dairy Cows in Bangladesh

“…34 However, they did not cure infection completely but reduced the antibody levels in treated cows recorded in this study are in conformity of the earlier reports. 34,40 The increased of antibody level at the 180 days of post-treatment in this study indicates that the combination of oxytetracycline and streptomycin did not eliminate the intra-cellular Brucella bacteria from treated animals. Therefore, shedding of Brucella organisms from treated animals should be monitored for longer duration, not only by PCR but also by bacteriological isolation and identification.…”

“…Lower value was recorded only with ALT in Brucella-affected cows in comparison to non-infected cows. These findings could be compared with the higher values of glucose, TSP and creatinine values and lower values of AST and ALT than the reference values (Kushwaha et al, 2014). However, significantly (p < 0.05) increased values of AST and ALT and non-significantly variation of creatinine in Brucella-infected cattle.…”

“…42 A large numbers of sero-prevalence studies on brucellosis in animals and humans have confirmed widespread prevalence of brucellosis in Bangladesh. 34 However, within reference value, decreased or even increased values of Hb in Brucella-affected cattle have been reported. 43 A significant decreased of TEC, Hb and PCV values while significantly increased value of TLC has been reported in Brucella-affected cattle.…”

Epidemiology and Biochemical Effects of Brucellosis in Kafer-El Sheikh and Qualyobia Cow's Farms

“…Also, [37] reported no significant changes in glucose level in brucella infected cows. On the other hand, [47] and [13] reported significant increase in creatinine level in brucella infected cattle and significant decrease in glucose level in brucella infected ewes respectively. Also, [36] found a significant correlation between CRP level and antibodies titre in patients infected with brucella.…”