Assessment of Interobserver Variability in Mitotic Figure Counting in Different Histological Grades of Oral Squamous Cell Carcinoma

Prasanna, MD; Yadav, K; Gonuguntla, Sudhir; Velidandla, Surekha; Reddy, CR Charan; Bommu, Sanjay Reddy

doi:10.5005/jp-journals-10024-1148

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…The detection of mitotic figures and determination of the mitotic rate per 10 high-power fields after staining with hematoxylin and eosin is an established marker of cellular proliferation in oncology 6 . The disadvantages of counting mitotic figures are the high inter- and intra-observer variation rates and limited reproducibility 10 , 11 . Another marker of cell proliferation proposed for the diagnosis of endometriosis is Ki-67 12 .…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Wetzk

Grübling

Forberger

et al. 2022

Geburtshilfe Frauenheilkd

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Objectives Endometriosis is a chronic disease which is diagnosed by surgical intervention combined with a histological work-up. Current international and national recommendations do not require the histological determination of the proliferation rate. The diagnostic and clinical importance of the mitotic rate in endometriotic lesions still remains to be elucidated. Methods In this retrospective study, the mitotic rates and clinical data of 542 patients with histologically diagnosed endometriosis were analyzed. The mean patient age was 33.5 ± 8.0 (17 – 72) years, and the mean reproductive lifespan was 21.2 ± 7.8 (4 – 41) years. Patients were divided into two groups and patientsʼ reproductive history and clinical endometriosis characteristics were compared between groups. The study group consisted of women with confirmed mitotic figures (n = 140, 25.83%) and the control group comprised women without proliferative activity according to their mitotic rates (n = 402, 74.27%). Results Women with endometriotic lesions and histologically confirmed mitotic figures were significantly more likely to have a higher endometriosis stage (p = 0.001), deep infiltrating endometriosis (p < 0.001), ovarian endometrioma (p = 0.012), and infertility (p = 0.049). A mitotic rate > 0 was seen significantly less often in cases with incidental findings of endometriosis (p = 0.031). The presence of symptoms and basic characteristics such as age, age at onset of menarche, reproductive lifespan and parity did not differ between the group with and the group without mitotic figures. Conclusion This study shows that a simple histological assessment of the mitotic rate offers additional diagnostic value for the detection of advanced stages of endometriosis. The possible role as a predictive marker for the recurrence of endometriosis or the development of endometriosis-associated cancer will require future study.

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Section: Introductionmentioning

confidence: 99%

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Wetzk

Grübling

Forberger

et al. 2022

Geburtshilfe Frauenheilkd

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“… Absent nuclear membrane 5,12–14 Hairy‐like extensions of nuclear material Shape of chromatin mass Typical Atypical …”

Section: Resultsmentioning

confidence: 99%

“…LM at 9400 magnification was used to identify mitoses. Features of mitosis and mimickers, as previously published, 6,[11][12][13] were applied to identify mitoses. To confirm mitosis and to differentiate between true mitosis and mimickers in challenging cases, phosphohistone H3 (PHH3) immunohistochemical (IHC) staining was used, as previously described.…”

Section: O M Pmentioning

confidence: 99%

Visual assessment of mitotic figures in breast cancer: a comparative study between light microscopy and whole slide images

et al. 2021

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Background and aims Visual assessment of mitotic figures in breast cancer (BC) remains a challenge. This is expected to be more pronounced in the digital pathology era. This study aims to refine the criteria of mitotic figure recognition, particularly in whole slide images (WSI). Method and results Haematoxylin and eosin (H&E)‐stained BC sections (n = 506) were examined using light microscopy (LM) and WSI. A set of features for identifying mitosis in WSI and to distinguish true figures from mimickers was developed. Changes in the mitotic count between the two platforms was explored. Morphological features of mitoses were recorded separately, including absence of nuclear membrane, chromatin hairy‐like projections, shape, cytoplasmic features, mitotic cell size and relationship to surrounding cells. Each mitotic phase has its own mimickers. Fifty‐eight per cent of mitoses showed absent hairy‐like projection in WSI; however, 89% retained their ragged nuclear border, which distinguished them from mimickers including apoptotic cells, lymphocytes and dark elongated hyperchromatic structures. Mitosis in WSI showed loss of fine details, and there was a 20% average reduction rate of mitotic counts when compared to the same area on LM. Using refined mitosis recognition criteria in WSI resulted in a twofold improvement of interobserver concordance. However, when compared to LM, 19% of cases were underscored in WSIs. Conclusions All morphological features of mitosis should be considered to enable recognition and differentiation from their mimickers, particularly in WSI, to ensure reliable BC grading. Refining mitotic cut‐offs per specific area when using WSI, based on the degree of reduction and association with outcome, is warranted.

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“…The cells touching the inner vertical line belonged to the 5th column and cells touching the inner horizontal line belonged to the second row. The counting begun at the upper left hand corner of the section and was moved vertically downwards along each section till chondrocytes were found [19]. At the end of the vertical direction of the section, the section was moved in the horizontal direction.…”

Section: Methodsmentioning

confidence: 99%

“…This was important in reproducing the results. Ten high-power fields were counted and the average was taken [19]. The cells were counted by a consultant pathologist.…”

Section: Methodsmentioning

confidence: 99%

Association between the Number of Chondrocytes of Lumbar Intervertebral Discs and Age, Abdominal Aorta Atherosclerosis and Lumbar Artery Arteriosclerosis Descriptive Cross Sectional Study

Karunanayake¹,

Gupta²

2015

Anat Physiol

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A n at omy & P h y s io logy: C u r r e n t Re sear c h ISSN: 2161-0940Karunanayake and Gupta, Anat Physiol 2015Physiol , 5:4 http://dx.doi.org/10.4172/2161 Keywords: Atherosclerosis; Intervertebral disc; Abdominal aorta; Chondrocytes IntroductionIt is estimated that in every population, an individual has an 80% probability of having low back pain at some period during their life time [1]. Low back pain is defined as a pain or discomfort in the lumbar sacral region [2]. Low back pain and lumbar sacral radicular pain have a large number of causes and out of those causes; intervertebral disc degeneration is one of the most common causes [3]. Intervertebral disc degeneration is an important cause of persistent low back pain with or without leg pain [4].The intervertebral disc is a soft tissue, positioned between each of the vertebrae to accommodate applied biomechanical forces to the spine. The central compartment of the disc contains the gelatinous nucleus pulposus, which is enclosed by the tough annulus fibrosus and the endplate cartilage [5]. The nucleus pulposus is highly pressurized and the annulus fibrosus prevents radial disc bulge which resists large tensile and compressive strains. The cartilaginous endplate is an interface tissue connecting the disc to the adjacent vertebral bodies, and functions to distribute stresses between the disc and the vertebrae and to act as a gateway for nutritional transport in the avascular disc [6]. Chondrocytes are the dominant cell type found in the disc and these cells are responsible for the synthesis, maintenance, and gradual turnover of the extracellular matrix. The cartilage extracellular matrix is remodeled in response to the functional demands of mechanical loading, and this process is mediated through the metabolic activity of chondrocytes [7]. Any structural defect and/or metabolic disturbances in the extracellular matrix may cause cellular and tissue alterations that can lead to the development or progression of disc degeneration [8].Degeneration of the intervertebral disc is a process characterized by a cascade of cellular, biochemical, structural and functional changes of the disc. A key factor in early disc degeneration is the decrease in proteoglycan content. [9]. During intervertebral disc degeneration (IVD), chondrocytes respond to early degeneration by increasing their biosynthetic processes [6]. Certain scientists believe that reimplantation of in vitro-activated autologous chondrocytes may be useful in reversing the disc degeneration process [10].In aged tissues, the extra cellular material is not efficiently maintained and it is believed that chondrocytes may exhibit altered mechano-responsive properties as they age. Another change observed with regards to age is that the stiffness of chondrocytes increases with advancing age [7]. In the above studies, what happens in regards to the number of chondrocytes with an increase in age has not been mentioned. AbstractBackground: The effect of age, atherosclerotic changes of abdominal aorta and arteriosclerot...

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Assessment of Interobserver Variability in Mitotic Figure Counting in Different Histological Grades of Oral Squamous Cell Carcinoma

Cited by 9 publications

References 8 publications

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Diagnostic Benefit of the Detection of Mitotic Figures in Endometriotic Lesions

Visual assessment of mitotic figures in breast cancer: a comparative study between light microscopy and whole slide images

Association between the Number of Chondrocytes of Lumbar Intervertebral Discs and Age, Abdominal Aorta Atherosclerosis and Lumbar Artery Arteriosclerosis Descriptive Cross Sectional Study

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