Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits.

Abstract

Microbial biota associated with wheat rhizosphere during flowering-stage and their plant growth promoting traits was investigated. 16S rRNA gene sequencing was performed of the isolates, which were obtained on selective media. Isolates belonged to: alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria; Actinobacteria; Bacteroidetes and Firmicutes. Bacillus was the most dominant genus (34.7%) followed by Pseudomonas (14.4%). Among diazotrophs, Arthrobacter sp. (n=3), Cupriavidus sp. (n=3) and Stenotrophomonas sp. (n=4) occurred more frequently. Most of the isolates produced indole acetic acid. Ac. baumannii , Ps. lini, Ser. marcescens, C. respiraculi and Ag.tumfaciens solubilized phosphate. Two Acinetobacter strains, five Pseudomonas srains and four Bacillus strains produced siderophore. Strains of Ps. aeruginosa, Ps. lini and Ps. thivervalensis exhibited in vitro fungal growth inhibition. Arthrobacter globiformis Y2S3 exhibited indole acetic acid production, siderophore production and antifungal activity. Plant growth promoting traits of these rhizobacteria indicated beneficial relationship between rhizobacteria and wheat plants. Several of the strains could be further developed as an effective bio-inoculant. Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits.

Temporal Variations in Diazotrophic Communities and nifH Transcripts Level Across the Agricultural and Fallow Land at Jaipur, Rajasthan, India

“…Azospirillum species are broadly distributed ecologically and are associated with vast varieties of plants including wheat, rice, maize and several non-leguminous crops [26] Ensifer adharenes was reported during the year 2011-2012 but this taxon did not appear again in rest of the study and previously reported in wheat cultivated soil samples [27]. Similarly, Frankia is a filamentous bacteria found in symbiotic association with non-leguminous crop, observed during the second year of study [28]. Indole acetic acid producing bacteria Ideonella sp.…”

Comparison of bacterial diversity, root exudates and soil enzymatic activities in the rhizosphere of AVP1-transgenic and nontransgenic wheat (Triticum aestivum L.)

“…Among the organic acids detected in the present study in soil solution, acetic acid was detected in relatively higher amounts followed by oxalic acid and citric acid. The presence of organic acids in the rhizosphere of different crops has been reported previously (Badri & Vivanco, 2009) and it has been found that rate of root exudates released by the plant roots influences microbial biota and activity in rhizosphere (Sachdev et al, 2009).…”